Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube

Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube

Z Sobhani1, R Dinarvand1,2, F Atyabi1,2, M Ghahremani3, M Adeli4,51Department of Pharmaceutics, 2Nanotechnology Research Center, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran; 4Department of Chemistry, Sharif University of Technology,...

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Autores principales: Sobhani Z, Dinarv, R, Atyabi F, Ghahremani M, Adeli M
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2011
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/ff02c21dcd3f43ebad7796f599d93ad9
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spelling oai:doaj.org-article:ff02c21dcd3f43ebad7796f599d93ad92021-12-02T01:23:32ZIncreased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube1176-91141178-2013https://doaj.org/article/ff02c21dcd3f43ebad7796f599d93ad92011-04-01T00:00:00Zhttp://www.dovepress.com/increased-paclitaxel-cytotoxicity-against-cancer-cell-lines-using-a-no-a6993https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Z Sobhani1, R Dinarvand1,2, F Atyabi1,2, M Ghahremani3, M Adeli4,51Department of Pharmaceutics, 2Nanotechnology Research Center, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran; 4Department of Chemistry, Sharif University of Technology, Tehran; 5Department of Chemistry, Faculty of Science, Lorestan University, Khoramabad, IranAbstract: Potential applications of carbon nanotubes have attracted many researchers in the field of drug delivery systems. In this study, multiwalled carbon nanotubes (MWNTs) were first functionalized using hyperbranched poly citric acid (PCA) to improve their hydrophilicity and functionality. Then, paclitaxel (PTX), a potent anticancer agent, was conjugated to the carboxyl functional groups of poly citric acid via a cleavable ester bond to obtain a MWNT-g-PCA-PTX conjugate. Drug content of the conjugate was about 38% (w/w). The particle size of MWNT-g-PCA and MWNT-g-PCA-PTX was approximately 125 and 200 nm, respectively. Atomic force microscopy and transmission electron microscopy images showed a curved shape for MWNT-g-PCA and MWNT-g-PCA-PTX, which was in contrast with the straight or linear conformation expected from carbon nanotubes. It seems that the high hydrophilicity of poly citric acid and high hydrophobicity of MWNTs led to conformational changes from a linear state to a curved state. Paclitaxel can be released from the MWNT-g-PCA-PTX conjugates faster at pH 6.8 and 5.0 than at pH 7.4, which was suitable for the release of the drug in tumor tissues and tumor cells. In vitro cytotoxicity studies were evaluated in the A549 and SKOV3 cell lines. MWNT-g-PCA had an insignificant cytotoxic effect on both cell lines. MWNT-g-PCA-PTX had more of a cytotoxic effect than the free drug over a shorter incubation time (eg, 24 hours versus 48 hours), which suggests improved cell penetration of MWNT-g-PCA-PTX. Therefore, paclitaxel conjugated to MWNT-g-PCA is promising for cancer therapeutics.Keywords: multiwalled carbon nanotubes, functionalization, anticancer, drug delivery, nanoparticles Sobhani ZDinarvRAtyabi FGhahremani MAdeli MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 705-719 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Sobhani Z
Dinarv
R
Atyabi F
Ghahremani M
Adeli M
Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube
description Z Sobhani1, R Dinarvand1,2, F Atyabi1,2, M Ghahremani3, M Adeli4,51Department of Pharmaceutics, 2Nanotechnology Research Center, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran; 4Department of Chemistry, Sharif University of Technology, Tehran; 5Department of Chemistry, Faculty of Science, Lorestan University, Khoramabad, IranAbstract: Potential applications of carbon nanotubes have attracted many researchers in the field of drug delivery systems. In this study, multiwalled carbon nanotubes (MWNTs) were first functionalized using hyperbranched poly citric acid (PCA) to improve their hydrophilicity and functionality. Then, paclitaxel (PTX), a potent anticancer agent, was conjugated to the carboxyl functional groups of poly citric acid via a cleavable ester bond to obtain a MWNT-g-PCA-PTX conjugate. Drug content of the conjugate was about 38% (w/w). The particle size of MWNT-g-PCA and MWNT-g-PCA-PTX was approximately 125 and 200 nm, respectively. Atomic force microscopy and transmission electron microscopy images showed a curved shape for MWNT-g-PCA and MWNT-g-PCA-PTX, which was in contrast with the straight or linear conformation expected from carbon nanotubes. It seems that the high hydrophilicity of poly citric acid and high hydrophobicity of MWNTs led to conformational changes from a linear state to a curved state. Paclitaxel can be released from the MWNT-g-PCA-PTX conjugates faster at pH 6.8 and 5.0 than at pH 7.4, which was suitable for the release of the drug in tumor tissues and tumor cells. In vitro cytotoxicity studies were evaluated in the A549 and SKOV3 cell lines. MWNT-g-PCA had an insignificant cytotoxic effect on both cell lines. MWNT-g-PCA-PTX had more of a cytotoxic effect than the free drug over a shorter incubation time (eg, 24 hours versus 48 hours), which suggests improved cell penetration of MWNT-g-PCA-PTX. Therefore, paclitaxel conjugated to MWNT-g-PCA is promising for cancer therapeutics.Keywords: multiwalled carbon nanotubes, functionalization, anticancer, drug delivery, nanoparticles
format article
author Sobhani Z
Dinarv
R
Atyabi F
Ghahremani M
Adeli M
author_facet Sobhani Z
Dinarv
R
Atyabi F
Ghahremani M
Adeli M
author_sort Sobhani Z
title Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube
title_short Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube
title_full Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube
title_fullStr Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube
title_full_unstemmed Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube
title_sort increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube
publisher Dove Medical Press
publishDate 2011
url https://doaj.org/article/ff02c21dcd3f43ebad7796f599d93ad9
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AT r increasedpaclitaxelcytotoxicityagainstcancercelllinesusinganovelfunctionalizedcarbonnanotube
AT atyabif increasedpaclitaxelcytotoxicityagainstcancercelllinesusinganovelfunctionalizedcarbonnanotube
AT ghahremanim increasedpaclitaxelcytotoxicityagainstcancercelllinesusinganovelfunctionalizedcarbonnanotube
AT adelim increasedpaclitaxelcytotoxicityagainstcancercelllinesusinganovelfunctionalizedcarbonnanotube
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