Quantum dot-based immunofluorescent imaging of Ki67 and identification of prognostic value in HER2-positive (non-luminal) breast cancer

Jin-Zhong Sun,1,* Chuang Chen,1,* Guan Jiang,2 Wei-Qun Tian,3 Yan Li,4 Sheng-Rong Sun1 1Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China; 2Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan, Hubei, Peo...

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Autores principales:	Sun JZ, Chen C, Jiang G, Tian WQ, Li Y, Sun SR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ff06d15109e54d54be2821c99b5badfc
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spelling	oai:doaj.org-article:ff06d15109e54d54be2821c99b5badfc2021-12-02T07:45:54ZQuantum dot-based immunofluorescent imaging of Ki67 and identification of prognostic value in HER2-positive (non-luminal) breast cancer1178-2013https://doaj.org/article/ff06d15109e54d54be2821c99b5badfc2014-03-01T00:00:00Zhttp://www.dovepress.com/quantum-dot-based-immunofluorescent-imaging-of-ki67-and-identification-a16072https://doaj.org/toc/1178-2013 Jin-Zhong Sun,1,* Chuang Chen,1,* Guan Jiang,2 Wei-Qun Tian,3 Yan Li,4 Sheng-Rong Sun1 1Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China; 2Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China; 3Department of Biomedical Engineering, Wuhan University School of Basic Medical Sciences, Wuhan, Hubei, People's Republic of China; 4Department of Oncology, Zhongnan Hospital of Wuhan University and Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuchang District, Wuhan, Hubei, People's Republic of China *These authors contributed equally to this work Background: The immunohistochemical assessment of Ki67 antigen (Ki67) is the most widely practiced measurement of breast cancer cell proliferation; however, it has some disadvantages and thus the prognostic value of Ki67 in breast cancer remains controversial. Our previous studies confirmed the advantages of quantum dots-based nanotechnology for quantitative analysis of biomarkers compared with conventional immunohistochemistry (IHC). This study was designed to assess Ki67 by quantum dot-immunohistochemistry (QD-IHC) and investigate the prognostic value of the Ki67 score in human epidermal growth factor receptor 2 (HER2)-positive (non-luminal) breast cancer. Methods: Ki67 expression in 108 HER2-positive (non-luminal) breast cancer specimens was detected by IHC and QD-IHC. Two observers assessed the Ki67 score independently and comparisons between the two methods were made. The prognostic value of the Ki67 score for five-year disease-free survival was estimated. Results: The same antigen localization, high correlation of staining rates (r=0.993), and high agreement of measurements (Κ=0.874) of Ki67 expression (cutoff: 30%) in breast cancer were found by QD-IHC and conventional IHC. The QD-IHC had a better interobserver agreement for the Ki67 score than conventional IHC (t=-7.280, P<0.01). High Ki67 expression (cutoff: 30%) was associated with shorter disease-free survival (log-rank test; IHC, P=0.026; QD-IHC, P=0.001), especially in the lymph node-negative subgroups (log-rank test; IHC, P=0.017; QD-IHC, P=0.002). Conclusion: QD-IHC imaging of Ki67 was an easier and more accurate method for detecting and assessing Ki67. The Ki67 score was an independent prognosticator in the HER2-positive (non-luminal) breast cancer patients. Keywords: quantum dots, breast cancer, Ki67, disease-free survival, prognosisSun JZChen CJiang GTian WQLi YSun SRDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1339-1346 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Sun JZ Chen C Jiang G Tian WQ Li Y Sun SR Quantum dot-based immunofluorescent imaging of Ki67 and identification of prognostic value in HER2-positive (non-luminal) breast cancer
description	Jin-Zhong Sun,1,* Chuang Chen,1,* Guan Jiang,2 Wei-Qun Tian,3 Yan Li,4 Sheng-Rong Sun1 1Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China; 2Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China; 3Department of Biomedical Engineering, Wuhan University School of Basic Medical Sciences, Wuhan, Hubei, People's Republic of China; 4Department of Oncology, Zhongnan Hospital of Wuhan University and Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuchang District, Wuhan, Hubei, People's Republic of China *These authors contributed equally to this work Background: The immunohistochemical assessment of Ki67 antigen (Ki67) is the most widely practiced measurement of breast cancer cell proliferation; however, it has some disadvantages and thus the prognostic value of Ki67 in breast cancer remains controversial. Our previous studies confirmed the advantages of quantum dots-based nanotechnology for quantitative analysis of biomarkers compared with conventional immunohistochemistry (IHC). This study was designed to assess Ki67 by quantum dot-immunohistochemistry (QD-IHC) and investigate the prognostic value of the Ki67 score in human epidermal growth factor receptor 2 (HER2)-positive (non-luminal) breast cancer. Methods: Ki67 expression in 108 HER2-positive (non-luminal) breast cancer specimens was detected by IHC and QD-IHC. Two observers assessed the Ki67 score independently and comparisons between the two methods were made. The prognostic value of the Ki67 score for five-year disease-free survival was estimated. Results: The same antigen localization, high correlation of staining rates (r=0.993), and high agreement of measurements (Κ=0.874) of Ki67 expression (cutoff: 30%) in breast cancer were found by QD-IHC and conventional IHC. The QD-IHC had a better interobserver agreement for the Ki67 score than conventional IHC (t=-7.280, P<0.01). High Ki67 expression (cutoff: 30%) was associated with shorter disease-free survival (log-rank test; IHC, P=0.026; QD-IHC, P=0.001), especially in the lymph node-negative subgroups (log-rank test; IHC, P=0.017; QD-IHC, P=0.002). Conclusion: QD-IHC imaging of Ki67 was an easier and more accurate method for detecting and assessing Ki67. The Ki67 score was an independent prognosticator in the HER2-positive (non-luminal) breast cancer patients. Keywords: quantum dots, breast cancer, Ki67, disease-free survival, prognosis
format	article
author	Sun JZ Chen C Jiang G Tian WQ Li Y Sun SR
author_facet	Sun JZ Chen C Jiang G Tian WQ Li Y Sun SR
author_sort	Sun JZ
title	Quantum dot-based immunofluorescent imaging of Ki67 and identification of prognostic value in HER2-positive (non-luminal) breast cancer
title_short	Quantum dot-based immunofluorescent imaging of Ki67 and identification of prognostic value in HER2-positive (non-luminal) breast cancer
title_full	Quantum dot-based immunofluorescent imaging of Ki67 and identification of prognostic value in HER2-positive (non-luminal) breast cancer
title_fullStr	Quantum dot-based immunofluorescent imaging of Ki67 and identification of prognostic value in HER2-positive (non-luminal) breast cancer
title_full_unstemmed	Quantum dot-based immunofluorescent imaging of Ki67 and identification of prognostic value in HER2-positive (non-luminal) breast cancer
title_sort	quantum dot-based immunofluorescent imaging of ki67 and identification of prognostic value in her2-positive (non-luminal) breast cancer
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/ff06d15109e54d54be2821c99b5badfc
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Quantum dot-based immunofluorescent imaging of Ki67 and identification of prognostic value in HER2-positive (non-luminal) breast cancer

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