Comparative geno-plasticity analysis of Mycoplasma bovis HB0801 (Chinese isolate).

Mycoplasma bovis pneumonia in cattle has been epidemic in China since 2008. To investigate M. bovis pathogenesis, we completed genome sequencing of strain HB0801 isolated from a lesioned bovine lung from Hubei, China. The genomic plasticity was determined by comparing HB0801 with M. bovis strain ATC...

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Autores principales:	Jingjing Qi, Aizhen Guo, Peng Cui, Yingyu Chen, Riaz Mustafa, Xiaoliang Ba, Changmin Hu, Zhidi Bai, Xi Chen, Lei Shi, Huanchun Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff1232b0acb94c99867d005bbbef5ae8
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spelling	oai:doaj.org-article:ff1232b0acb94c99867d005bbbef5ae82021-11-18T07:16:40ZComparative geno-plasticity analysis of Mycoplasma bovis HB0801 (Chinese isolate).1932-620310.1371/journal.pone.0038239https://doaj.org/article/ff1232b0acb94c99867d005bbbef5ae82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22693604/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Mycoplasma bovis pneumonia in cattle has been epidemic in China since 2008. To investigate M. bovis pathogenesis, we completed genome sequencing of strain HB0801 isolated from a lesioned bovine lung from Hubei, China. The genomic plasticity was determined by comparing HB0801 with M. bovis strain ATCC® 25523™/PG45 from cow mastitis milk, Chinese strain Hubei-1 from lesioned lung tissue, and 16 other Mycoplasmas species. Compared to PG45, the genome size of HB0801 was reduced by 11.7 kb. Furthermore, a large chromosome inversion (580 kb) was confirmed in all Chinese isolates including HB0801, HB1007, a strain from cow mastitis milk, and Hubei-1. In addition, the variable surface lipoproteins (vsp) gene cluster existed in HB0801, but contained less than half of the genes, and had poor identity to that in PG45, but they had conserved structures. Further inter-strain comparisons revealed other mechanisms of gene acquisition and loss in HB0801 that primarily involved insertion sequence (IS) elements, integrative conjugative element, restriction and modification systems, and some lipoproteins and transmembrane proteins. Subsequently, PG45 and HB0801 virulence in cattle was compared. Results indicated that both strains were pathogenic to cattle. The scores of gross pathological assessment for the control group, and the PG45- and HB0801-infected groups were 3, 13 and 9, respectively. Meanwhile the scores of lung lesion for these three groups were 36, 70, and 69, respectively. In addition, immunohistochemistry detection demonstrated that both strains were similarly distributed in lungs and lymph nodes. Although PG45 showed slightly higher virulence in calves than HB0801, there was no statistical difference between the strains (P>0.05). Compared to Hubei-1, a total of 122 SNP loci were disclosed in HB0801. In conclusion, although genomic plasticity was thought to be an evolutionary advantage, it did not apparently affect virulence of M. bovis strains in cattle.Jingjing QiAizhen GuoPeng CuiYingyu ChenRiaz MustafaXiaoliang BaChangmin HuZhidi BaiXi ChenLei ShiHuanchun ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e38239 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jingjing Qi Aizhen Guo Peng Cui Yingyu Chen Riaz Mustafa Xiaoliang Ba Changmin Hu Zhidi Bai Xi Chen Lei Shi Huanchun Chen Comparative geno-plasticity analysis of Mycoplasma bovis HB0801 (Chinese isolate).
description	Mycoplasma bovis pneumonia in cattle has been epidemic in China since 2008. To investigate M. bovis pathogenesis, we completed genome sequencing of strain HB0801 isolated from a lesioned bovine lung from Hubei, China. The genomic plasticity was determined by comparing HB0801 with M. bovis strain ATCC® 25523™/PG45 from cow mastitis milk, Chinese strain Hubei-1 from lesioned lung tissue, and 16 other Mycoplasmas species. Compared to PG45, the genome size of HB0801 was reduced by 11.7 kb. Furthermore, a large chromosome inversion (580 kb) was confirmed in all Chinese isolates including HB0801, HB1007, a strain from cow mastitis milk, and Hubei-1. In addition, the variable surface lipoproteins (vsp) gene cluster existed in HB0801, but contained less than half of the genes, and had poor identity to that in PG45, but they had conserved structures. Further inter-strain comparisons revealed other mechanisms of gene acquisition and loss in HB0801 that primarily involved insertion sequence (IS) elements, integrative conjugative element, restriction and modification systems, and some lipoproteins and transmembrane proteins. Subsequently, PG45 and HB0801 virulence in cattle was compared. Results indicated that both strains were pathogenic to cattle. The scores of gross pathological assessment for the control group, and the PG45- and HB0801-infected groups were 3, 13 and 9, respectively. Meanwhile the scores of lung lesion for these three groups were 36, 70, and 69, respectively. In addition, immunohistochemistry detection demonstrated that both strains were similarly distributed in lungs and lymph nodes. Although PG45 showed slightly higher virulence in calves than HB0801, there was no statistical difference between the strains (P>0.05). Compared to Hubei-1, a total of 122 SNP loci were disclosed in HB0801. In conclusion, although genomic plasticity was thought to be an evolutionary advantage, it did not apparently affect virulence of M. bovis strains in cattle.
format	article
author	Jingjing Qi Aizhen Guo Peng Cui Yingyu Chen Riaz Mustafa Xiaoliang Ba Changmin Hu Zhidi Bai Xi Chen Lei Shi Huanchun Chen
author_facet	Jingjing Qi Aizhen Guo Peng Cui Yingyu Chen Riaz Mustafa Xiaoliang Ba Changmin Hu Zhidi Bai Xi Chen Lei Shi Huanchun Chen
author_sort	Jingjing Qi
title	Comparative geno-plasticity analysis of Mycoplasma bovis HB0801 (Chinese isolate).
title_short	Comparative geno-plasticity analysis of Mycoplasma bovis HB0801 (Chinese isolate).
title_full	Comparative geno-plasticity analysis of Mycoplasma bovis HB0801 (Chinese isolate).
title_fullStr	Comparative geno-plasticity analysis of Mycoplasma bovis HB0801 (Chinese isolate).
title_full_unstemmed	Comparative geno-plasticity analysis of Mycoplasma bovis HB0801 (Chinese isolate).
title_sort	comparative geno-plasticity analysis of mycoplasma bovis hb0801 (chinese isolate).
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ff1232b0acb94c99867d005bbbef5ae8
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Comparative geno-plasticity analysis of Mycoplasma bovis HB0801 (Chinese isolate).

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