Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases

The proliferation of antibiotic resistance has its origins in horizontal gene transfer. The class 1 integrons mediate gene transfer by assimilating antibiotic-resistance genes through site-specific recombination. For the class 1 integrons the first assimilated gene normally encodes an aminoglycoside...

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Autores principales:	Jun Zhang, Getong Liu, Xuhui Zhang, Yaowen Chang, Shasha Wang, Weizhi He, Wenxia Sun, Dongrong Chen, Alastair I.H. Murchie
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	aminoglycoside riboswitch adenyltransferase antibiotic resistance Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/ff16ce90bb8a4f588f08c269fbee32b0
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spelling	oai:doaj.org-article:ff16ce90bb8a4f588f08c269fbee32b02021-11-17T14:21:59ZAminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases2150-55942150-560810.1080/21505594.2020.1836910https://doaj.org/article/ff16ce90bb8a4f588f08c269fbee32b02020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1836910https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608The proliferation of antibiotic resistance has its origins in horizontal gene transfer. The class 1 integrons mediate gene transfer by assimilating antibiotic-resistance genes through site-specific recombination. For the class 1 integrons the first assimilated gene normally encodes an aminoglycoside antibiotic resistance protein which is either an aminoglycoside acetyltransferase (AAC), nucleotidyltransferase – (ANT), or adenyl transferase (AAD). An aminoglycoside-sensing riboswitch RNA in the leader RNA of AAC/AAD that controls the expression of aminoglycoside resistance genes has been previously described. Here we explore the relationship between the recombinant products of integron recombination and a series of candidate riboswitch RNAs in the 5ʹ UTR of aad (aminoglycoside adenyltransferases) genes. The RNA sequences from the 5ʹ UTR of the aad genes from pathogenic strains that are the products of site-specific DNA recombination by class 1 integrons were investigated. Reporter assays, MicroScale Thermophoresis (MST) and covariance analysis revealed that a functional aminoglycoside-sensing riboswitch was selected at the DNA level through integron-mediated site-specific recombination. This study explains the close association between integron recombination and the aminoglycoside-sensing riboswitch RNA.Jun ZhangGetong LiuXuhui ZhangYaowen ChangShasha WangWeizhi HeWenxia SunDongrong ChenAlastair I.H. MurchieTaylor & Francis Grouparticleaminoglycosideriboswitchadenyltransferaseantibiotic resistanceInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1432-1442 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	aminoglycoside riboswitch adenyltransferase antibiotic resistance Infectious and parasitic diseases RC109-216
spellingShingle	aminoglycoside riboswitch adenyltransferase antibiotic resistance Infectious and parasitic diseases RC109-216 Jun Zhang Getong Liu Xuhui Zhang Yaowen Chang Shasha Wang Weizhi He Wenxia Sun Dongrong Chen Alastair I.H. Murchie Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases
description	The proliferation of antibiotic resistance has its origins in horizontal gene transfer. The class 1 integrons mediate gene transfer by assimilating antibiotic-resistance genes through site-specific recombination. For the class 1 integrons the first assimilated gene normally encodes an aminoglycoside antibiotic resistance protein which is either an aminoglycoside acetyltransferase (AAC), nucleotidyltransferase – (ANT), or adenyl transferase (AAD). An aminoglycoside-sensing riboswitch RNA in the leader RNA of AAC/AAD that controls the expression of aminoglycoside resistance genes has been previously described. Here we explore the relationship between the recombinant products of integron recombination and a series of candidate riboswitch RNAs in the 5ʹ UTR of aad (aminoglycoside adenyltransferases) genes. The RNA sequences from the 5ʹ UTR of the aad genes from pathogenic strains that are the products of site-specific DNA recombination by class 1 integrons were investigated. Reporter assays, MicroScale Thermophoresis (MST) and covariance analysis revealed that a functional aminoglycoside-sensing riboswitch was selected at the DNA level through integron-mediated site-specific recombination. This study explains the close association between integron recombination and the aminoglycoside-sensing riboswitch RNA.
format	article
author	Jun Zhang Getong Liu Xuhui Zhang Yaowen Chang Shasha Wang Weizhi He Wenxia Sun Dongrong Chen Alastair I.H. Murchie
author_facet	Jun Zhang Getong Liu Xuhui Zhang Yaowen Chang Shasha Wang Weizhi He Wenxia Sun Dongrong Chen Alastair I.H. Murchie
author_sort	Jun Zhang
title	Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases
title_short	Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases
title_full	Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases
title_fullStr	Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases
title_full_unstemmed	Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases
title_sort	aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/ff16ce90bb8a4f588f08c269fbee32b0
work_keys_str_mv	AT junzhang aminoglycosideriboswitchcontroloftheexpressionofintegronassociatedaminoglycosideresistanceadenyltransferases AT getongliu aminoglycosideriboswitchcontroloftheexpressionofintegronassociatedaminoglycosideresistanceadenyltransferases AT xuhuizhang aminoglycosideriboswitchcontroloftheexpressionofintegronassociatedaminoglycosideresistanceadenyltransferases AT yaowenchang aminoglycosideriboswitchcontroloftheexpressionofintegronassociatedaminoglycosideresistanceadenyltransferases AT shashawang aminoglycosideriboswitchcontroloftheexpressionofintegronassociatedaminoglycosideresistanceadenyltransferases AT weizhihe aminoglycosideriboswitchcontroloftheexpressionofintegronassociatedaminoglycosideresistanceadenyltransferases AT wenxiasun aminoglycosideriboswitchcontroloftheexpressionofintegronassociatedaminoglycosideresistanceadenyltransferases AT dongrongchen aminoglycosideriboswitchcontroloftheexpressionofintegronassociatedaminoglycosideresistanceadenyltransferases AT alastairihmurchie aminoglycosideriboswitchcontroloftheexpressionofintegronassociatedaminoglycosideresistanceadenyltransferases
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Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases

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