GLP-1 Inhibits High-Glucose-Induced Oxidative Injury of Vascular Endothelial Cells

Abstract The aim of this work was to evaluate the effects of glucagon-like peptide-1 (GLP-1) on high-glucose-induced oxidative stress and investigate the possible mechanisms underlying this process. We measured reactive oxygen species (ROS) production, cell apoptosis, the expression of NOX4 and its...

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Autores principales:	Quan Li, Yajun Lin, Shu Wang, Lina Zhang, Lixin Guo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff1d0372db3c4ce8b14d24d2ba11d675
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spelling	oai:doaj.org-article:ff1d0372db3c4ce8b14d24d2ba11d6752021-12-02T15:05:25ZGLP-1 Inhibits High-Glucose-Induced Oxidative Injury of Vascular Endothelial Cells10.1038/s41598-017-06712-z2045-2322https://doaj.org/article/ff1d0372db3c4ce8b14d24d2ba11d6752017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06712-zhttps://doaj.org/toc/2045-2322Abstract The aim of this work was to evaluate the effects of glucagon-like peptide-1 (GLP-1) on high-glucose-induced oxidative stress and investigate the possible mechanisms underlying this process. We measured reactive oxygen species (ROS) production, cell apoptosis, the expression of NOX4 and its subunits, and p47phox translocation in human umbilical vein endothelial cells (HUVECs). An experimental type 2 diabetes model was induced using streptozotocin in male Sprague-Dawley rats. Fasting blood glucose (FBG), fasting insulin (FINS), total cholesterol (TC), triglycerides (TGs), and free fatty acid (FFA) were measured. Histomorphological analysis of the aorta was performed using hematoxylin-eosin staining. NOX4 and VCAM-1 expression in the aorta was measured. We found that high-glucose-induced ROS production and apoptosis were inhibited by GLP-1 treatment. High glucose caused upregulation of NOX4, p47phox, and Rac-1 and translocation of p47phox but had no effect on the cells pretreated with GLP-1. Furthermore, in the diabetic group, FBG, FINS, TG, TC, and FFA were increased, and NOX4 and VCAM-1 levels were also elevated. However, GLP-1 attenuated all these changes. GLP-1 ameliorated high-glucose-induced oxidative stress by inhibiting NOX4, p47phox, and Rac-1 expression and translocation of p47phox, suggesting its clinical usefulness in diabetic vascular complications.Quan LiYajun LinShu WangLina ZhangLixin GuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Quan Li Yajun Lin Shu Wang Lina Zhang Lixin Guo GLP-1 Inhibits High-Glucose-Induced Oxidative Injury of Vascular Endothelial Cells
description	Abstract The aim of this work was to evaluate the effects of glucagon-like peptide-1 (GLP-1) on high-glucose-induced oxidative stress and investigate the possible mechanisms underlying this process. We measured reactive oxygen species (ROS) production, cell apoptosis, the expression of NOX4 and its subunits, and p47phox translocation in human umbilical vein endothelial cells (HUVECs). An experimental type 2 diabetes model was induced using streptozotocin in male Sprague-Dawley rats. Fasting blood glucose (FBG), fasting insulin (FINS), total cholesterol (TC), triglycerides (TGs), and free fatty acid (FFA) were measured. Histomorphological analysis of the aorta was performed using hematoxylin-eosin staining. NOX4 and VCAM-1 expression in the aorta was measured. We found that high-glucose-induced ROS production and apoptosis were inhibited by GLP-1 treatment. High glucose caused upregulation of NOX4, p47phox, and Rac-1 and translocation of p47phox but had no effect on the cells pretreated with GLP-1. Furthermore, in the diabetic group, FBG, FINS, TG, TC, and FFA were increased, and NOX4 and VCAM-1 levels were also elevated. However, GLP-1 attenuated all these changes. GLP-1 ameliorated high-glucose-induced oxidative stress by inhibiting NOX4, p47phox, and Rac-1 expression and translocation of p47phox, suggesting its clinical usefulness in diabetic vascular complications.
format	article
author	Quan Li Yajun Lin Shu Wang Lina Zhang Lixin Guo
author_facet	Quan Li Yajun Lin Shu Wang Lina Zhang Lixin Guo
author_sort	Quan Li
title	GLP-1 Inhibits High-Glucose-Induced Oxidative Injury of Vascular Endothelial Cells
title_short	GLP-1 Inhibits High-Glucose-Induced Oxidative Injury of Vascular Endothelial Cells
title_full	GLP-1 Inhibits High-Glucose-Induced Oxidative Injury of Vascular Endothelial Cells
title_fullStr	GLP-1 Inhibits High-Glucose-Induced Oxidative Injury of Vascular Endothelial Cells
title_full_unstemmed	GLP-1 Inhibits High-Glucose-Induced Oxidative Injury of Vascular Endothelial Cells
title_sort	glp-1 inhibits high-glucose-induced oxidative injury of vascular endothelial cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ff1d0372db3c4ce8b14d24d2ba11d675
work_keys_str_mv	AT quanli glp1inhibitshighglucoseinducedoxidativeinjuryofvascularendothelialcells AT yajunlin glp1inhibitshighglucoseinducedoxidativeinjuryofvascularendothelialcells AT shuwang glp1inhibitshighglucoseinducedoxidativeinjuryofvascularendothelialcells AT linazhang glp1inhibitshighglucoseinducedoxidativeinjuryofvascularendothelialcells AT lixinguo glp1inhibitshighglucoseinducedoxidativeinjuryofvascularendothelialcells
_version_	1718388870139084800

GLP-1 Inhibits High-Glucose-Induced Oxidative Injury of Vascular Endothelial Cells

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