Controlling T cells spreading, mechanics and activation by micropatterning

Abstract We designed a strategy, based on a careful examination of the activation capabilities of proteins and antibodies used as substrates for adhering T cells, coupled to protein microstamping to control at the same time the position, shape, spreading, mechanics and activation state of T cells. O...

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Autores principales:	Anaïs Sadoun, Martine Biarnes-Pelicot, Laura Ghesquiere-Dierickx, Ambroise Wu, Olivier Théodoly, Laurent Limozin, Yannick Hamon, Pierre-Henri Puech
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff1e48ef684a4b078137f5fce273a6b2
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spelling	oai:doaj.org-article:ff1e48ef684a4b078137f5fce273a6b22021-12-02T16:36:04ZControlling T cells spreading, mechanics and activation by micropatterning10.1038/s41598-021-86133-12045-2322https://doaj.org/article/ff1e48ef684a4b078137f5fce273a6b22021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86133-1https://doaj.org/toc/2045-2322Abstract We designed a strategy, based on a careful examination of the activation capabilities of proteins and antibodies used as substrates for adhering T cells, coupled to protein microstamping to control at the same time the position, shape, spreading, mechanics and activation state of T cells. Once adhered on patterns, we examined the capacities of T cells to be activated with soluble anti CD3, in comparison to T cells adhered to a continuously decorated substrate with the same density of ligands. We show that, in our hand, adhering onto an anti CD45 antibody decorated surface was not affecting T cell calcium fluxes, even adhered on variable size micro-patterns. Aside, we analyzed the T cell mechanics, when spread on pattern or not, using Atomic Force Microscopy indentation. By expressing MEGF10 as a non immune adhesion receptor in T cells we measured the very same spreading area on PLL substrates and Young modulus than non modified cells, immobilized on anti CD45 antibodies, while retaining similar activation capabilities using soluble anti CD3 antibodies or through model APC contacts. We propose that our system is a way to test activation or anergy of T cells with defined adhesion and mechanical characteristics, and may allow to dissect fine details of these mechanisms since it allows to observe homogenized populations in standardized T cell activation assays.Anaïs SadounMartine Biarnes-PelicotLaura Ghesquiere-DierickxAmbroise WuOlivier ThéodolyLaurent LimozinYannick HamonPierre-Henri PuechNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anaïs Sadoun Martine Biarnes-Pelicot Laura Ghesquiere-Dierickx Ambroise Wu Olivier Théodoly Laurent Limozin Yannick Hamon Pierre-Henri Puech Controlling T cells spreading, mechanics and activation by micropatterning
description	Abstract We designed a strategy, based on a careful examination of the activation capabilities of proteins and antibodies used as substrates for adhering T cells, coupled to protein microstamping to control at the same time the position, shape, spreading, mechanics and activation state of T cells. Once adhered on patterns, we examined the capacities of T cells to be activated with soluble anti CD3, in comparison to T cells adhered to a continuously decorated substrate with the same density of ligands. We show that, in our hand, adhering onto an anti CD45 antibody decorated surface was not affecting T cell calcium fluxes, even adhered on variable size micro-patterns. Aside, we analyzed the T cell mechanics, when spread on pattern or not, using Atomic Force Microscopy indentation. By expressing MEGF10 as a non immune adhesion receptor in T cells we measured the very same spreading area on PLL substrates and Young modulus than non modified cells, immobilized on anti CD45 antibodies, while retaining similar activation capabilities using soluble anti CD3 antibodies or through model APC contacts. We propose that our system is a way to test activation or anergy of T cells with defined adhesion and mechanical characteristics, and may allow to dissect fine details of these mechanisms since it allows to observe homogenized populations in standardized T cell activation assays.
format	article
author	Anaïs Sadoun Martine Biarnes-Pelicot Laura Ghesquiere-Dierickx Ambroise Wu Olivier Théodoly Laurent Limozin Yannick Hamon Pierre-Henri Puech
author_facet	Anaïs Sadoun Martine Biarnes-Pelicot Laura Ghesquiere-Dierickx Ambroise Wu Olivier Théodoly Laurent Limozin Yannick Hamon Pierre-Henri Puech
author_sort	Anaïs Sadoun
title	Controlling T cells spreading, mechanics and activation by micropatterning
title_short	Controlling T cells spreading, mechanics and activation by micropatterning
title_full	Controlling T cells spreading, mechanics and activation by micropatterning
title_fullStr	Controlling T cells spreading, mechanics and activation by micropatterning
title_full_unstemmed	Controlling T cells spreading, mechanics and activation by micropatterning
title_sort	controlling t cells spreading, mechanics and activation by micropatterning
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ff1e48ef684a4b078137f5fce273a6b2
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Controlling T cells spreading, mechanics and activation by micropatterning

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