Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa

Sumaira Anjum, Bilal Haider Abbasi Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan Abstract: In vitro-derived cultures of plants offer a great potential for rapid biosynthesis of chemical-free antimicrobial silver nanoparticles (AgNPs) by enhancing their phytoche...

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Autores principales:	Anjum S, Abbasi BH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Silver nanoparticles Phlomis bracteosa In vitro micropropagation Antimicrobial Multidrug resistant bacteria. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ff20420bc3ed4916966a73a26a54d221
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spelling	oai:doaj.org-article:ff20420bc3ed4916966a73a26a54d2212021-12-02T00:37:19ZBiomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa1178-2013https://doaj.org/article/ff20420bc3ed4916966a73a26a54d2212016-04-01T00:00:00Zhttps://www.dovepress.com/biomimetic-synthesis-of-antimicrobial-silver-nanoparticles-using-in-vi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sumaira Anjum, Bilal Haider Abbasi Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan Abstract: In vitro-derived cultures of plants offer a great potential for rapid biosynthesis of chemical-free antimicrobial silver nanoparticles (AgNPs) by enhancing their phytochemical reducing potential. Here, we developed an efficient protocol for in vitro micropropagation of a high-value endangered medicinal plant species, Phlomis bracteosa, in order to explore its biogenic potential in biomimetic synthesis of antimicrobial AgNPs. Murashige and Skoog medium supplemented with 2.0 mg/L thidiazuron was found to be more efficient in inducing optimum in vitro shoot regeneration (78%±4.09%), and 2.0 mg/L indole-3-butyric acid was used for maximum root induction (86%±4.457%). Antimicrobial AgNPs were successfully synthesized by using aqueous extract (rich in total phenolics and flavonoids content) of in vitro derived plantlets of P. bracteosa. Ultraviolet–visible spectroscopy of synthesized AgNPs showed characteristic surface plasmon band in the range of 420–429 nm. The crystallinity, size, and shape of the AgNPs were characterized by X-ray diffraction and scanning electron microscopy. Face-centered cubic AgNPs of almost uniform spherical size (22.41 nm) were synthesized within a short time (1 hour) at room temperature. Fourier-transform infrared spectroscopy revealed that the polyphenols were mainly responsible for reduction and capping of synthesized AgNPs. Energy dispersive X-ray analysis further endorsed the presence of elemental silver in synthesized AgNPs. These biosynthesized AgNPs displayed significantly higher bactericidal activity against multiple drug-resistant human pathogens. The present work highlighted the potent role of in vitro-derived plantlets of P. bracteosa for feasible biosynthesis of antimicrobial AgNPs, which can be used as nanomedicines in many biomedical applications. Keywords: silver nanoparticles, Phlomis bracteosa, in vitro micropropagation, antimicrobial, multidrug resistant bacteriaAnjum SAbbasi BHDove Medical PressarticleSilver nanoparticlesPhlomis bracteosaIn vitro micropropagationAntimicrobialMultidrug resistant bacteria.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1663-1675 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Silver nanoparticles Phlomis bracteosa In vitro micropropagation Antimicrobial Multidrug resistant bacteria. Medicine (General) R5-920
spellingShingle	Silver nanoparticles Phlomis bracteosa In vitro micropropagation Antimicrobial Multidrug resistant bacteria. Medicine (General) R5-920 Anjum S Abbasi BH Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa
description	Sumaira Anjum, Bilal Haider Abbasi Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan Abstract: In vitro-derived cultures of plants offer a great potential for rapid biosynthesis of chemical-free antimicrobial silver nanoparticles (AgNPs) by enhancing their phytochemical reducing potential. Here, we developed an efficient protocol for in vitro micropropagation of a high-value endangered medicinal plant species, Phlomis bracteosa, in order to explore its biogenic potential in biomimetic synthesis of antimicrobial AgNPs. Murashige and Skoog medium supplemented with 2.0 mg/L thidiazuron was found to be more efficient in inducing optimum in vitro shoot regeneration (78%±4.09%), and 2.0 mg/L indole-3-butyric acid was used for maximum root induction (86%±4.457%). Antimicrobial AgNPs were successfully synthesized by using aqueous extract (rich in total phenolics and flavonoids content) of in vitro derived plantlets of P. bracteosa. Ultraviolet–visible spectroscopy of synthesized AgNPs showed characteristic surface plasmon band in the range of 420–429 nm. The crystallinity, size, and shape of the AgNPs were characterized by X-ray diffraction and scanning electron microscopy. Face-centered cubic AgNPs of almost uniform spherical size (22.41 nm) were synthesized within a short time (1 hour) at room temperature. Fourier-transform infrared spectroscopy revealed that the polyphenols were mainly responsible for reduction and capping of synthesized AgNPs. Energy dispersive X-ray analysis further endorsed the presence of elemental silver in synthesized AgNPs. These biosynthesized AgNPs displayed significantly higher bactericidal activity against multiple drug-resistant human pathogens. The present work highlighted the potent role of in vitro-derived plantlets of P. bracteosa for feasible biosynthesis of antimicrobial AgNPs, which can be used as nanomedicines in many biomedical applications. Keywords: silver nanoparticles, Phlomis bracteosa, in vitro micropropagation, antimicrobial, multidrug resistant bacteria
format	article
author	Anjum S Abbasi BH
author_facet	Anjum S Abbasi BH
author_sort	Anjum S
title	Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa
title_short	Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa
title_full	Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa
title_fullStr	Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa
title_full_unstemmed	Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa
title_sort	biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: phlomis bracteosa
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/ff20420bc3ed4916966a73a26a54d221
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Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa

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