Environmental biodegradability of recombinant structural protein

Abstract Next generation polymers needs to be produced from renewable sources and to be converted into inorganic compounds in the natural environment at the end of life. Recombinant structural protein is a promising alternative to conventional engineering plastics due to its good thermal and mechani...

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Autores principales:	Yuya Tachibana, Sunita Darbe, Senri Hayashi, Alina Kudasheva, Haruna Misawa, Yuka Shibata, Ken-ichi Kasuya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff222339fc6a4b0895ea493d847c0ed6
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spelling	oai:doaj.org-article:ff222339fc6a4b0895ea493d847c0ed62021-12-02T11:45:54ZEnvironmental biodegradability of recombinant structural protein10.1038/s41598-020-80114-62045-2322https://doaj.org/article/ff222339fc6a4b0895ea493d847c0ed62021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80114-6https://doaj.org/toc/2045-2322Abstract Next generation polymers needs to be produced from renewable sources and to be converted into inorganic compounds in the natural environment at the end of life. Recombinant structural protein is a promising alternative to conventional engineering plastics due to its good thermal and mechanical properties, its production from biomass, and its potential for biodegradability. Herein, we measured the thermal and mechanical properties of the recombinant structural protein BP1 and evaluated its biodegradability. Because the thermal degradation occurs above 250 °C and the glass transition temperature is 185 °C, BP1 can be molded into sheets by a manual hot press at 150 °C and 83 MPa. The flexural strength and modulus of BP1 were 115 ± 6 MPa and 7.38 ± 0.03 GPa. These properties are superior to those of commercially available biodegradable polymers. The biodegradability of BP1 was carefully evaluated. BP1 was shown to be efficiently hydrolyzed by some isolated bacterial strains in a dispersed state. Furthermore, it was readily hydrolyzed from the solid state by three isolated proteases. The mineralization was evaluated by the biochemical oxygen demand (BOD)-biodegradation testing with soil inocula. The BOD biodegradability of BP1 was 70.2 ± 6.0 after 33 days.Yuya TachibanaSunita DarbeSenri HayashiAlina KudashevaHaruna MisawaYuka ShibataKen-ichi KasuyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuya Tachibana Sunita Darbe Senri Hayashi Alina Kudasheva Haruna Misawa Yuka Shibata Ken-ichi Kasuya Environmental biodegradability of recombinant structural protein
description	Abstract Next generation polymers needs to be produced from renewable sources and to be converted into inorganic compounds in the natural environment at the end of life. Recombinant structural protein is a promising alternative to conventional engineering plastics due to its good thermal and mechanical properties, its production from biomass, and its potential for biodegradability. Herein, we measured the thermal and mechanical properties of the recombinant structural protein BP1 and evaluated its biodegradability. Because the thermal degradation occurs above 250 °C and the glass transition temperature is 185 °C, BP1 can be molded into sheets by a manual hot press at 150 °C and 83 MPa. The flexural strength and modulus of BP1 were 115 ± 6 MPa and 7.38 ± 0.03 GPa. These properties are superior to those of commercially available biodegradable polymers. The biodegradability of BP1 was carefully evaluated. BP1 was shown to be efficiently hydrolyzed by some isolated bacterial strains in a dispersed state. Furthermore, it was readily hydrolyzed from the solid state by three isolated proteases. The mineralization was evaluated by the biochemical oxygen demand (BOD)-biodegradation testing with soil inocula. The BOD biodegradability of BP1 was 70.2 ± 6.0 after 33 days.
format	article
author	Yuya Tachibana Sunita Darbe Senri Hayashi Alina Kudasheva Haruna Misawa Yuka Shibata Ken-ichi Kasuya
author_facet	Yuya Tachibana Sunita Darbe Senri Hayashi Alina Kudasheva Haruna Misawa Yuka Shibata Ken-ichi Kasuya
author_sort	Yuya Tachibana
title	Environmental biodegradability of recombinant structural protein
title_short	Environmental biodegradability of recombinant structural protein
title_full	Environmental biodegradability of recombinant structural protein
title_fullStr	Environmental biodegradability of recombinant structural protein
title_full_unstemmed	Environmental biodegradability of recombinant structural protein
title_sort	environmental biodegradability of recombinant structural protein
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ff222339fc6a4b0895ea493d847c0ed6
work_keys_str_mv	AT yuyatachibana environmentalbiodegradabilityofrecombinantstructuralprotein AT sunitadarbe environmentalbiodegradabilityofrecombinantstructuralprotein AT senrihayashi environmentalbiodegradabilityofrecombinantstructuralprotein AT alinakudasheva environmentalbiodegradabilityofrecombinantstructuralprotein AT harunamisawa environmentalbiodegradabilityofrecombinantstructuralprotein AT yukashibata environmentalbiodegradabilityofrecombinantstructuralprotein AT kenichikasuya environmentalbiodegradabilityofrecombinantstructuralprotein
_version_	1718395262007771136

Environmental biodegradability of recombinant structural protein

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