Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.

<h4>Background</h4>Fluoride (F) is a naturally exists in nature but several studies have indicated it as an environmental toxicant to all leaving beings. Human F exposure has increased over the years since this ion has been used by industry on foods, beverages, toothpastes and on water s...

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Autores principales:	Bruna Puty, Leonardo Oliveira Bittencourt, Iago Cesar Nogueira, Marília Afonso Rabelo Buzalaf, Edivaldo Herculano Oliveira, Rafael Rodrigues Lima
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff255360520a4fc9b4e9d4b8311aae6f
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spelling	oai:doaj.org-article:ff255360520a4fc9b4e9d4b8311aae6f2021-11-25T06:23:31ZHuman cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.1932-620310.1371/journal.pone.0251200https://doaj.org/article/ff255360520a4fc9b4e9d4b8311aae6f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251200https://doaj.org/toc/1932-6203<h4>Background</h4>Fluoride (F) is a naturally exists in nature but several studies have indicated it as an environmental toxicant to all leaving beings. Human F exposure has increased over the years since this ion has been used by industry on foods, beverages, toothpastes and on water supply. Although F is safe at optimal concentrations in water supply, human exposure to high levels could trigger neurofunctional deficits.<h4>Materials and methods</h4>In this study, human glial-like (U87) and neuronal-like (IMR-32) cells lineages were used to access F toxicity and CNS cell sensibility on both cell facing the same protocol. Cells were exposed to F over 3, 5 and 10 days on two different F concentrations. Fluoride exposed cells were evaluated by standard toxicity assays to cell viability, apoptosis, necrosis and general cell metabolism. Oxidative stress parameters were evaluated by ATP and ROS levels, lipid peroxidation, GSH/GSSG ratio and comet assay.<h4>Results</h4>No changes were observed in IMR-32 at any given time while after 10 days of exposure to 0.22μg/mL, U87 glial-like cells showed signs of toxicity such as decreased cell viability by necrosis while general cell metabolism was increased. Oxidative stress parameters were next evaluated only on U87 glial-like cells after 10 days of exposure. F induced a decrease on ATP levels while no changes were observed on reactive oxygen species and lipid peroxidation. GSH/GSSG ratio was decreased followed by DNA damage both on 0.22μg/mL F.<h4>Conclusions</h4>Our results suggest an important differential behavior of the distinct types of cells exposed to the different fluoride concentrations, pointing that the U87 glial-like cells as more susceptible to damage triggered by this ion.Bruna PutyLeonardo Oliveira BittencourtIago Cesar NogueiraMarília Afonso Rabelo BuzalafEdivaldo Herculano OliveiraRafael Rodrigues LimaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0251200 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bruna Puty Leonardo Oliveira Bittencourt Iago Cesar Nogueira Marília Afonso Rabelo Buzalaf Edivaldo Herculano Oliveira Rafael Rodrigues Lima Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.
description	<h4>Background</h4>Fluoride (F) is a naturally exists in nature but several studies have indicated it as an environmental toxicant to all leaving beings. Human F exposure has increased over the years since this ion has been used by industry on foods, beverages, toothpastes and on water supply. Although F is safe at optimal concentrations in water supply, human exposure to high levels could trigger neurofunctional deficits.<h4>Materials and methods</h4>In this study, human glial-like (U87) and neuronal-like (IMR-32) cells lineages were used to access F toxicity and CNS cell sensibility on both cell facing the same protocol. Cells were exposed to F over 3, 5 and 10 days on two different F concentrations. Fluoride exposed cells were evaluated by standard toxicity assays to cell viability, apoptosis, necrosis and general cell metabolism. Oxidative stress parameters were evaluated by ATP and ROS levels, lipid peroxidation, GSH/GSSG ratio and comet assay.<h4>Results</h4>No changes were observed in IMR-32 at any given time while after 10 days of exposure to 0.22μg/mL, U87 glial-like cells showed signs of toxicity such as decreased cell viability by necrosis while general cell metabolism was increased. Oxidative stress parameters were next evaluated only on U87 glial-like cells after 10 days of exposure. F induced a decrease on ATP levels while no changes were observed on reactive oxygen species and lipid peroxidation. GSH/GSSG ratio was decreased followed by DNA damage both on 0.22μg/mL F.<h4>Conclusions</h4>Our results suggest an important differential behavior of the distinct types of cells exposed to the different fluoride concentrations, pointing that the U87 glial-like cells as more susceptible to damage triggered by this ion.
format	article
author	Bruna Puty Leonardo Oliveira Bittencourt Iago Cesar Nogueira Marília Afonso Rabelo Buzalaf Edivaldo Herculano Oliveira Rafael Rodrigues Lima
author_facet	Bruna Puty Leonardo Oliveira Bittencourt Iago Cesar Nogueira Marília Afonso Rabelo Buzalaf Edivaldo Herculano Oliveira Rafael Rodrigues Lima
author_sort	Bruna Puty
title	Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.
title_short	Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.
title_full	Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.
title_fullStr	Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.
title_full_unstemmed	Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.
title_sort	human cultured imr-32 neuronal-like and u87 glial-like cells have different patterns of toxicity under fluoride exposure.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ff255360520a4fc9b4e9d4b8311aae6f
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_version_	1718413841498374144

Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.

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