Identification and comparative expression analysis of interleukin 2/15 receptor β chain in chickens infected with E. tenella.

<h4>Background</h4>Interleukin (IL) 2 and IL15 receptor β chain (IL2/15Rβ, CD122) play critical roles in signal transduction for the biological activities of IL2 and IL15. Increased knowledge of non-mammalian IL2/15Rβ will enhance the understanding of IL2 and IL15 functions.<h4>Met...

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Autores principales:	Jipseol Jeong, Woo H Kim, Jeongmi Yoo, Changhwan Lee, Suk Kim, Jae-Hyeon Cho, Hyung-Kwan Jang, Dong W Kim, Hyun S Lillehoj, Wongi Min
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff2625b20d4948738d0990eb040df3cf
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spelling	oai:doaj.org-article:ff2625b20d4948738d0990eb040df3cf2021-11-18T07:17:20ZIdentification and comparative expression analysis of interleukin 2/15 receptor β chain in chickens infected with E. tenella.1932-620310.1371/journal.pone.0037704https://doaj.org/article/ff2625b20d4948738d0990eb040df3cf2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22662196/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Interleukin (IL) 2 and IL15 receptor β chain (IL2/15Rβ, CD122) play critical roles in signal transduction for the biological activities of IL2 and IL15. Increased knowledge of non-mammalian IL2/15Rβ will enhance the understanding of IL2 and IL15 functions.<h4>Methodology/principal findings</h4>[corrected] Chicken IL2/15Rβ (chIL2/15Rβ) cDNA was cloned using 5'/3'-RACE. The predicted protein sequence contained 576 amino acids and typical features of the type-I cytokine receptor family. COS-7 cells transfected with chIL2/15Rβ produced proteins of approximately 75 and 62.5 kDa under normal and tunicamycin-treated conditions, respectively. The genomic structure of chIL2/15Rβ was similar to its mammalian counterparts. chIL2/15Rβ transcripts were detected in the lymphoblast cell line CU205 and in normal lymphoid organs and at moderate levels in bursa samples. Expression profiles of chIL2/15Rβ and its related cytokines and receptors were examined in ConA-stimulated splenic lymphocytes and in ceca-tonsils of Eimeria tenella-infected chickens using quantitative real-time PCR. Expression levels of chIL2/15Rβ, chIL2Rα, and chIL15Rα were generally elevated in ceca-tonsils and ConA-activated splenic lymphocytes. However, chIL2 and chIL15 expression levels were differentially regulated between the samples. chIL2 expression was upregulated in ConA-activated splenic lymphocytes, but not in ceca-tonsils. In constrast, chIL15 expression was upregulated in ceca-tonsils, but not in ConA-activated splenic lymphocytes.<h4>Conclusions/significance</h4>We identified an avian form of IL2/15Rβ and compared its gene expression pattern with those of chIL2, chIL15, chIL2Rα, and chIL15Rα. Our observations suggest that chIL15 and its receptors, including chIL2/15Rβ, play important roles in mucosal immunity to intestinal intracellular parasites such as Eimeria.Jipseol JeongWoo H KimJeongmi YooChanghwan LeeSuk KimJae-Hyeon ChoHyung-Kwan JangDong W KimHyun S LillehojWongi MinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37704 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jipseol Jeong Woo H Kim Jeongmi Yoo Changhwan Lee Suk Kim Jae-Hyeon Cho Hyung-Kwan Jang Dong W Kim Hyun S Lillehoj Wongi Min Identification and comparative expression analysis of interleukin 2/15 receptor β chain in chickens infected with E. tenella.
description	<h4>Background</h4>Interleukin (IL) 2 and IL15 receptor β chain (IL2/15Rβ, CD122) play critical roles in signal transduction for the biological activities of IL2 and IL15. Increased knowledge of non-mammalian IL2/15Rβ will enhance the understanding of IL2 and IL15 functions.<h4>Methodology/principal findings</h4>[corrected] Chicken IL2/15Rβ (chIL2/15Rβ) cDNA was cloned using 5'/3'-RACE. The predicted protein sequence contained 576 amino acids and typical features of the type-I cytokine receptor family. COS-7 cells transfected with chIL2/15Rβ produced proteins of approximately 75 and 62.5 kDa under normal and tunicamycin-treated conditions, respectively. The genomic structure of chIL2/15Rβ was similar to its mammalian counterparts. chIL2/15Rβ transcripts were detected in the lymphoblast cell line CU205 and in normal lymphoid organs and at moderate levels in bursa samples. Expression profiles of chIL2/15Rβ and its related cytokines and receptors were examined in ConA-stimulated splenic lymphocytes and in ceca-tonsils of Eimeria tenella-infected chickens using quantitative real-time PCR. Expression levels of chIL2/15Rβ, chIL2Rα, and chIL15Rα were generally elevated in ceca-tonsils and ConA-activated splenic lymphocytes. However, chIL2 and chIL15 expression levels were differentially regulated between the samples. chIL2 expression was upregulated in ConA-activated splenic lymphocytes, but not in ceca-tonsils. In constrast, chIL15 expression was upregulated in ceca-tonsils, but not in ConA-activated splenic lymphocytes.<h4>Conclusions/significance</h4>We identified an avian form of IL2/15Rβ and compared its gene expression pattern with those of chIL2, chIL15, chIL2Rα, and chIL15Rα. Our observations suggest that chIL15 and its receptors, including chIL2/15Rβ, play important roles in mucosal immunity to intestinal intracellular parasites such as Eimeria.
format	article
author	Jipseol Jeong Woo H Kim Jeongmi Yoo Changhwan Lee Suk Kim Jae-Hyeon Cho Hyung-Kwan Jang Dong W Kim Hyun S Lillehoj Wongi Min
author_facet	Jipseol Jeong Woo H Kim Jeongmi Yoo Changhwan Lee Suk Kim Jae-Hyeon Cho Hyung-Kwan Jang Dong W Kim Hyun S Lillehoj Wongi Min
author_sort	Jipseol Jeong
title	Identification and comparative expression analysis of interleukin 2/15 receptor β chain in chickens infected with E. tenella.
title_short	Identification and comparative expression analysis of interleukin 2/15 receptor β chain in chickens infected with E. tenella.
title_full	Identification and comparative expression analysis of interleukin 2/15 receptor β chain in chickens infected with E. tenella.
title_fullStr	Identification and comparative expression analysis of interleukin 2/15 receptor β chain in chickens infected with E. tenella.
title_full_unstemmed	Identification and comparative expression analysis of interleukin 2/15 receptor β chain in chickens infected with E. tenella.
title_sort	identification and comparative expression analysis of interleukin 2/15 receptor β chain in chickens infected with e. tenella.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ff2625b20d4948738d0990eb040df3cf
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Identification and comparative expression analysis of interleukin 2/15 receptor β chain in chickens infected with E. tenella.

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