Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages.
HIV-1-containing internal compartments are readily detected in images of thin sections from infected cells using conventional transmission electron microscopy, but the origin, connectivity, and 3D distribution of these compartments has remained controversial. Here, we report the 3D distribution of v...
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2009
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oai:doaj.org-article:ff291b2f78df48c08df4bba89ac931952021-11-25T05:47:36ZIon-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages.1553-73661553-737410.1371/journal.ppat.1000591https://doaj.org/article/ff291b2f78df48c08df4bba89ac931952009-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19779568/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374HIV-1-containing internal compartments are readily detected in images of thin sections from infected cells using conventional transmission electron microscopy, but the origin, connectivity, and 3D distribution of these compartments has remained controversial. Here, we report the 3D distribution of viruses in HIV-1-infected primary human macrophages using cryo-electron tomography and ion-abrasion scanning electron microscopy (IA-SEM), a recently developed approach for nanoscale 3D imaging of whole cells. Using IA-SEM, we show the presence of an extensive network of HIV-1-containing tubular compartments in infected macrophages, with diameters of approximately 150-200 nm, and lengths of up to approximately 5 microm that extend to the cell surface from vesicular compartments that contain assembling HIV-1 virions. These types of surface-connected tubular compartments are not observed in T cells infected with the 29/31 KE Gag-matrix mutant where the virus is targeted to multi-vesicular bodies and released into the extracellular medium. IA-SEM imaging also allows visualization of large sheet-like structures that extend outward from the surfaces of macrophages, which may bend and fold back to allow continual creation of viral compartments and virion-lined channels. This potential mechanism for efficient virus trafficking between the cell surface and interior may represent a subversion of pre-existing vesicular machinery for antigen capture, processing, sequestration, and presentation.Adam E BennettKedar NarayanDan ShiLisa M HartnellKarine GoussetHaifeng HeBradley C LowekampTerry S YooDonald BlissEric O FreedSriram SubramaniamPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 9, p e1000591 (2009) |
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DOAJ |
| collection |
DOAJ |
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EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Adam E Bennett Kedar Narayan Dan Shi Lisa M Hartnell Karine Gousset Haifeng He Bradley C Lowekamp Terry S Yoo Donald Bliss Eric O Freed Sriram Subramaniam Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages. |
| description |
HIV-1-containing internal compartments are readily detected in images of thin sections from infected cells using conventional transmission electron microscopy, but the origin, connectivity, and 3D distribution of these compartments has remained controversial. Here, we report the 3D distribution of viruses in HIV-1-infected primary human macrophages using cryo-electron tomography and ion-abrasion scanning electron microscopy (IA-SEM), a recently developed approach for nanoscale 3D imaging of whole cells. Using IA-SEM, we show the presence of an extensive network of HIV-1-containing tubular compartments in infected macrophages, with diameters of approximately 150-200 nm, and lengths of up to approximately 5 microm that extend to the cell surface from vesicular compartments that contain assembling HIV-1 virions. These types of surface-connected tubular compartments are not observed in T cells infected with the 29/31 KE Gag-matrix mutant where the virus is targeted to multi-vesicular bodies and released into the extracellular medium. IA-SEM imaging also allows visualization of large sheet-like structures that extend outward from the surfaces of macrophages, which may bend and fold back to allow continual creation of viral compartments and virion-lined channels. This potential mechanism for efficient virus trafficking between the cell surface and interior may represent a subversion of pre-existing vesicular machinery for antigen capture, processing, sequestration, and presentation. |
| format |
article |
| author |
Adam E Bennett Kedar Narayan Dan Shi Lisa M Hartnell Karine Gousset Haifeng He Bradley C Lowekamp Terry S Yoo Donald Bliss Eric O Freed Sriram Subramaniam |
| author_facet |
Adam E Bennett Kedar Narayan Dan Shi Lisa M Hartnell Karine Gousset Haifeng He Bradley C Lowekamp Terry S Yoo Donald Bliss Eric O Freed Sriram Subramaniam |
| author_sort |
Adam E Bennett |
| title |
Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages. |
| title_short |
Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages. |
| title_full |
Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages. |
| title_fullStr |
Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages. |
| title_full_unstemmed |
Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages. |
| title_sort |
ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in hiv-infected macrophages. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2009 |
| url |
https://doaj.org/article/ff291b2f78df48c08df4bba89ac93195 |
| work_keys_str_mv |
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