Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study

Abstract Background Hypha essential genes (HEGs) of Candida Albicans have been emerging into scholar’s attention, little known about their functions in oral lichen planus (OLP) with an uncovered etiology. This research aimed to observe necessary genes in biphasic C. albicans from OLP and study their...

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Autores principales:	Hong He, Ying Wang, Yan Fan, Congcong Li, Jianxin Han
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Bimorphysim switch Candida albicans Hypha/mycelium essential genes Oral lichen planus Pathogenesis Etiology Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/ff299dc6accf400c9fd0f786cf2775e2
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spelling	oai:doaj.org-article:ff299dc6accf400c9fd0f786cf2775e22021-12-05T12:20:35ZHypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study10.1186/s12903-021-01975-51472-6831https://doaj.org/article/ff299dc6accf400c9fd0f786cf2775e22021-12-01T00:00:00Zhttps://doi.org/10.1186/s12903-021-01975-5https://doaj.org/toc/1472-6831Abstract Background Hypha essential genes (HEGs) of Candida Albicans have been emerging into scholar’s attention, little known about their functions in oral lichen planus (OLP) with an uncovered etiology. This research aimed to observe necessary genes in biphasic C. albicans from OLP and study their relevance in pathogenesis, so as to evaluate possible roles of morphologic switching in etiology of OLP. Methods Samples were collected from OLP lesions of patients, mycelia were cultured and total RNA was extracted then subjected to reverse transcription-PCR and real-time PCR. Results HWP1 and HGC1 were significantly expressed in hyphae phase and weakly detected in yeast phase, while there was no significant difference of EFG1, ALS3, and ECE1 between in yeast and mycelia. Conclusion HGC1 and HWP1 were confirmed to be hypha essential genes, with HGC1 for hypha morphogenesis and HWP1 for adhesion invasion in pathogenesis of C. albicans in OLP. ALS3, ECE1 and EFG1 played minor roles in hyphae maintenance and adhesion for hyphae. These might be deemed as hints for the etiology of OLP and indicate HGC1 and HWP1 to be a priority of potential drug target.Hong HeYing WangYan FanCongcong LiJianxin HanBMCarticleBimorphysim switchCandida albicansHypha/mycelium essential genesOral lichen planusPathogenesisEtiologyDentistryRK1-715ENBMC Oral Health, Vol 21, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bimorphysim switch Candida albicans Hypha/mycelium essential genes Oral lichen planus Pathogenesis Etiology Dentistry RK1-715
spellingShingle	Bimorphysim switch Candida albicans Hypha/mycelium essential genes Oral lichen planus Pathogenesis Etiology Dentistry RK1-715 Hong He Ying Wang Yan Fan Congcong Li Jianxin Han Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study
description	Abstract Background Hypha essential genes (HEGs) of Candida Albicans have been emerging into scholar’s attention, little known about their functions in oral lichen planus (OLP) with an uncovered etiology. This research aimed to observe necessary genes in biphasic C. albicans from OLP and study their relevance in pathogenesis, so as to evaluate possible roles of morphologic switching in etiology of OLP. Methods Samples were collected from OLP lesions of patients, mycelia were cultured and total RNA was extracted then subjected to reverse transcription-PCR and real-time PCR. Results HWP1 and HGC1 were significantly expressed in hyphae phase and weakly detected in yeast phase, while there was no significant difference of EFG1, ALS3, and ECE1 between in yeast and mycelia. Conclusion HGC1 and HWP1 were confirmed to be hypha essential genes, with HGC1 for hypha morphogenesis and HWP1 for adhesion invasion in pathogenesis of C. albicans in OLP. ALS3, ECE1 and EFG1 played minor roles in hyphae maintenance and adhesion for hyphae. These might be deemed as hints for the etiology of OLP and indicate HGC1 and HWP1 to be a priority of potential drug target.
format	article
author	Hong He Ying Wang Yan Fan Congcong Li Jianxin Han
author_facet	Hong He Ying Wang Yan Fan Congcong Li Jianxin Han
author_sort	Hong He
title	Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study
title_short	Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study
title_full	Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study
title_fullStr	Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study
title_full_unstemmed	Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study
title_sort	hypha essential genes in candida albicans pathogenesis of oral lichen planus: an in-vitro study
publisher	BMC
publishDate	2021
url	https://doaj.org/article/ff299dc6accf400c9fd0f786cf2775e2
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Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study

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