Effects of transection of cervical sympathetic trunk on cognitive function of traumatic brain injury rats

Juan Long,1 Chunjing He,1 Hong Dai,2 Xinguo Kang,1 Jinfeng Zou,1 Shengli Ye,1 Qian Yu21Department of Pain, Guizhou Province People‘s Hospital, Guiyang, People’s Republic of China; 2Department of Neurology, Guizhou Province People‘s Hospital, Guiyang, People’s...

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Autores principales:	Long J, He CJ, Dai H, Kang XG, Zou JF, Ye SL, Yu Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Traumatic brain injury Transection of the cervical sympathetic trunk Sympathetic nervous system Inflammatory response Mitochondrial function Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/ff2a37844daa4e2cb7d523c77ec93b67
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spelling	oai:doaj.org-article:ff2a37844daa4e2cb7d523c77ec93b672021-12-02T05:50:15ZEffects of transection of cervical sympathetic trunk on cognitive function of traumatic brain injury rats1178-2021https://doaj.org/article/ff2a37844daa4e2cb7d523c77ec93b672019-05-01T00:00:00Zhttps://www.dovepress.com/effects-of-transection-of-cervical-sympathetic-trunk-on-cognitive-func-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Juan Long,1 Chunjing He,1 Hong Dai,2 Xinguo Kang,1 Jinfeng Zou,1 Shengli Ye,1 Qian Yu21Department of Pain, Guizhou Province People‘s Hospital, Guiyang, People’s Republic of China; 2Department of Neurology, Guizhou Province People‘s Hospital, Guiyang, People’s Republic of ChinaObjective: To observe the effects of transection of cervical sympathetic trunk (TCST) on the cognitive function of traumatic brain injury (TBI) rats and the potential mechanisms.Methods: A total of 288 adult male SD rats were divided into 3 groups using a random number table: TBI group (n=96), TBI + TCST group (n=96) and Sham group (n=96). The water maze test was performed before TBI (T0) and at day 1 (T1), day 2 (T2), day 3 (T3), 1 week (T4), 2 weeks (T5), 6 weeks (T6) and 12 weeks (T7) after TBI. The levels of α1-adrenergic receptors (α1-ARs), α2-adrenergic receptors (α2-ARs), toll-like receptor 4 (TLR-4) and P38 in hippocampi were detected by real-time PCR. Hippocampal P38 expression was assayed by Western blot. The expressions of interleukin-6 (IL-6), tumor necrosis factor (TNF-α) and brain-derived neurotrophic factor (BDNF) were examined by immunohistochemistry. Noradrenaline (NE) expression in plasma was evaluated by ELISA. The respiratory control ratio (RCR) of brain mitochondria was detected using a Clark oxygen electrode.Results: TCST effectively improved the cognitive function of TBI rats. TCST significantly inhibited sympathetic activity in the rats and effectively inhibited inflammatory responses. The expression of BDNF at T1-T6 in TBI+TCST group was higher than that in TBI group (P<0.05). Furthermore, P38 expression was inhibited more effectively in TBI+TCST group (P<0.05), than in TBI group (P<0.05), and the RCR of the brain was significantly higher in TBI+TCST group than in TBI group (P<0.05).Conclusions: TCST can enhance cognitive function in TBI rats by inhibiting sympathetic activity, reducing inflammatory responses and brain edema, upregulating BDNF and improving brain mitochondrial function.Keywords: traumatic brain injury, transection of the cervical sympathetic trunk, sympathetic nervous system, inflammatory response, mitochondrial functionLong JHe CJDai HKang XGZou JFYe SLYu QDove Medical PressarticleTraumatic brain injuryTransection of the cervical sympathetic trunkSympathetic nervous systemInflammatory responseMitochondrial functionNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 1121-1131 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Traumatic brain injury Transection of the cervical sympathetic trunk Sympathetic nervous system Inflammatory response Mitochondrial function Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Traumatic brain injury Transection of the cervical sympathetic trunk Sympathetic nervous system Inflammatory response Mitochondrial function Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Long J He CJ Dai H Kang XG Zou JF Ye SL Yu Q Effects of transection of cervical sympathetic trunk on cognitive function of traumatic brain injury rats
description	Juan Long,1 Chunjing He,1 Hong Dai,2 Xinguo Kang,1 Jinfeng Zou,1 Shengli Ye,1 Qian Yu21Department of Pain, Guizhou Province People‘s Hospital, Guiyang, People’s Republic of China; 2Department of Neurology, Guizhou Province People‘s Hospital, Guiyang, People’s Republic of ChinaObjective: To observe the effects of transection of cervical sympathetic trunk (TCST) on the cognitive function of traumatic brain injury (TBI) rats and the potential mechanisms.Methods: A total of 288 adult male SD rats were divided into 3 groups using a random number table: TBI group (n=96), TBI + TCST group (n=96) and Sham group (n=96). The water maze test was performed before TBI (T0) and at day 1 (T1), day 2 (T2), day 3 (T3), 1 week (T4), 2 weeks (T5), 6 weeks (T6) and 12 weeks (T7) after TBI. The levels of α1-adrenergic receptors (α1-ARs), α2-adrenergic receptors (α2-ARs), toll-like receptor 4 (TLR-4) and P38 in hippocampi were detected by real-time PCR. Hippocampal P38 expression was assayed by Western blot. The expressions of interleukin-6 (IL-6), tumor necrosis factor (TNF-α) and brain-derived neurotrophic factor (BDNF) were examined by immunohistochemistry. Noradrenaline (NE) expression in plasma was evaluated by ELISA. The respiratory control ratio (RCR) of brain mitochondria was detected using a Clark oxygen electrode.Results: TCST effectively improved the cognitive function of TBI rats. TCST significantly inhibited sympathetic activity in the rats and effectively inhibited inflammatory responses. The expression of BDNF at T1-T6 in TBI+TCST group was higher than that in TBI group (P<0.05). Furthermore, P38 expression was inhibited more effectively in TBI+TCST group (P<0.05), than in TBI group (P<0.05), and the RCR of the brain was significantly higher in TBI+TCST group than in TBI group (P<0.05).Conclusions: TCST can enhance cognitive function in TBI rats by inhibiting sympathetic activity, reducing inflammatory responses and brain edema, upregulating BDNF and improving brain mitochondrial function.Keywords: traumatic brain injury, transection of the cervical sympathetic trunk, sympathetic nervous system, inflammatory response, mitochondrial function
format	article
author	Long J He CJ Dai H Kang XG Zou JF Ye SL Yu Q
author_facet	Long J He CJ Dai H Kang XG Zou JF Ye SL Yu Q
author_sort	Long J
title	Effects of transection of cervical sympathetic trunk on cognitive function of traumatic brain injury rats
title_short	Effects of transection of cervical sympathetic trunk on cognitive function of traumatic brain injury rats
title_full	Effects of transection of cervical sympathetic trunk on cognitive function of traumatic brain injury rats
title_fullStr	Effects of transection of cervical sympathetic trunk on cognitive function of traumatic brain injury rats
title_full_unstemmed	Effects of transection of cervical sympathetic trunk on cognitive function of traumatic brain injury rats
title_sort	effects of transection of cervical sympathetic trunk on cognitive function of traumatic brain injury rats
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/ff2a37844daa4e2cb7d523c77ec93b67
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Effects of transection of cervical sympathetic trunk on cognitive function of traumatic brain injury rats

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