Anti-inflammatory and angiogenic effects of exercise training in cardiac muscle of diabetic mice

Tom L Broderick,1 Jacqueline M Sennott,2 Jolanta Gutkowska,3 Marek Jankowski31Laboratory of Diabetes and Exercise Metabolism, Department of Physiology, College of Graduate Studies, Midwestern University, Glendale, AZ, USA; 2Department of Cardiology, Medical Education H23, Saint-Joseph Mercy Health S...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Broderick TL, Sennott JM, Gutkowska J, Jankowski M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	exercise db/db glycated hemoglobin angiogenesis inflammation Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/ff2c801d0d0044f28f421113290efaf9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ff2c801d0d0044f28f421113290efaf9
record_format	dspace
spelling	oai:doaj.org-article:ff2c801d0d0044f28f421113290efaf92021-12-02T03:22:05ZAnti-inflammatory and angiogenic effects of exercise training in cardiac muscle of diabetic mice1178-7007https://doaj.org/article/ff2c801d0d0044f28f421113290efaf92019-04-01T00:00:00Zhttps://www.dovepress.com/anti-inflammatory-and-angiogenic-effects-of-exercise-training-in-cardi-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Tom L Broderick,1 Jacqueline M Sennott,2 Jolanta Gutkowska,3 Marek Jankowski31Laboratory of Diabetes and Exercise Metabolism, Department of Physiology, College of Graduate Studies, Midwestern University, Glendale, AZ, USA; 2Department of Cardiology, Medical Education H23, Saint-Joseph Mercy Health System, Pontiac, MI, USA; 3Cardiovascular Biochemistry Laboratory, CRCHUM (7-134), Department of Medicine, University of Montreal, Montreal, Quebec, CanadaBackground: Improved glycemic control and cardiovascular function are major benefits of regular exercise training (ET) in type 2 diabetes. Recent work has demonstrated that ET improves cardiac and vascular functions independent of obesity, inflammation, and glucose control in the diabetic db/db mouse. In this study, we determined whether ET can overcome the effects of elevated inflammatory cytokines and hyperglycemia on markers of cardiac angiogenesis and inflammation in the diabetic mouse.Methods: Male diabetic db/db mice were assigned to a sedentary and exercise-trained group. Sedentary lean control littermates were used as controls. ET was performed at moderate intensity on a treadmill 5 days a week for a period of 8 weeks. After ET, blood was collected for assay of glucose, hemoglobin (HB and HB1AC), C-reactive protein (CRP), and IL-6. Markers of inflammation and insulin resistance (IL-6, IL-1β, and tumor necrosis factor-alpha [TNF-α]) and angiogenesis (endothelial nitric oxide synthase [eNOS], vascular endothelial growth factor-A [VEGF-A], and hypoxia-inducible factor-1α [HIF-1α]) were measured in hearts.Results: Diabetic db/db mice remained obese and hyperglycemic after ET. Percent total HB and HB1AC were significantly higher in ET db/db mice compared to sedentary db/db mice, indicating further deterioration of glucose control with ET. Plasma levels of CRP and IL-6 were higher in sedentary db/db mice compared to control mice and were unaffected by ET. However, in the presence of hyperglycemia and elevated plasma cytokines, protein expression of eNOS, mRNA expression of VEGF-A, and HIF-1α was increased in db/db hearts after ET. On the other hand, protein expression of TNF-α and mRNA expression IL-6 and IL-1β was significantly decreased by ET in hearts of db/db mice.Conclusion: Our results indicate that ET improves cardiac markers of angiogenesis, insulin resistance, and endothelial dysfunction in the db/db mouse. This was observed independently of obesity, hyperglycemia, and the systemic inflammatory state.Keywords: exercise, db/db, glycated hemoglobin, angiogenesis, inflammationBroderick TLSennott JMGutkowska JJankowski MDove Medical Pressarticleexercisedb/dbglycated hemoglobinangiogenesisinflammationSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 565-573 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	exercise db/db glycated hemoglobin angiogenesis inflammation Specialties of internal medicine RC581-951
spellingShingle	exercise db/db glycated hemoglobin angiogenesis inflammation Specialties of internal medicine RC581-951 Broderick TL Sennott JM Gutkowska J Jankowski M Anti-inflammatory and angiogenic effects of exercise training in cardiac muscle of diabetic mice
description	Tom L Broderick,1 Jacqueline M Sennott,2 Jolanta Gutkowska,3 Marek Jankowski31Laboratory of Diabetes and Exercise Metabolism, Department of Physiology, College of Graduate Studies, Midwestern University, Glendale, AZ, USA; 2Department of Cardiology, Medical Education H23, Saint-Joseph Mercy Health System, Pontiac, MI, USA; 3Cardiovascular Biochemistry Laboratory, CRCHUM (7-134), Department of Medicine, University of Montreal, Montreal, Quebec, CanadaBackground: Improved glycemic control and cardiovascular function are major benefits of regular exercise training (ET) in type 2 diabetes. Recent work has demonstrated that ET improves cardiac and vascular functions independent of obesity, inflammation, and glucose control in the diabetic db/db mouse. In this study, we determined whether ET can overcome the effects of elevated inflammatory cytokines and hyperglycemia on markers of cardiac angiogenesis and inflammation in the diabetic mouse.Methods: Male diabetic db/db mice were assigned to a sedentary and exercise-trained group. Sedentary lean control littermates were used as controls. ET was performed at moderate intensity on a treadmill 5 days a week for a period of 8 weeks. After ET, blood was collected for assay of glucose, hemoglobin (HB and HB1AC), C-reactive protein (CRP), and IL-6. Markers of inflammation and insulin resistance (IL-6, IL-1β, and tumor necrosis factor-alpha [TNF-α]) and angiogenesis (endothelial nitric oxide synthase [eNOS], vascular endothelial growth factor-A [VEGF-A], and hypoxia-inducible factor-1α [HIF-1α]) were measured in hearts.Results: Diabetic db/db mice remained obese and hyperglycemic after ET. Percent total HB and HB1AC were significantly higher in ET db/db mice compared to sedentary db/db mice, indicating further deterioration of glucose control with ET. Plasma levels of CRP and IL-6 were higher in sedentary db/db mice compared to control mice and were unaffected by ET. However, in the presence of hyperglycemia and elevated plasma cytokines, protein expression of eNOS, mRNA expression of VEGF-A, and HIF-1α was increased in db/db hearts after ET. On the other hand, protein expression of TNF-α and mRNA expression IL-6 and IL-1β was significantly decreased by ET in hearts of db/db mice.Conclusion: Our results indicate that ET improves cardiac markers of angiogenesis, insulin resistance, and endothelial dysfunction in the db/db mouse. This was observed independently of obesity, hyperglycemia, and the systemic inflammatory state.Keywords: exercise, db/db, glycated hemoglobin, angiogenesis, inflammation
format	article
author	Broderick TL Sennott JM Gutkowska J Jankowski M
author_facet	Broderick TL Sennott JM Gutkowska J Jankowski M
author_sort	Broderick TL
title	Anti-inflammatory and angiogenic effects of exercise training in cardiac muscle of diabetic mice
title_short	Anti-inflammatory and angiogenic effects of exercise training in cardiac muscle of diabetic mice
title_full	Anti-inflammatory and angiogenic effects of exercise training in cardiac muscle of diabetic mice
title_fullStr	Anti-inflammatory and angiogenic effects of exercise training in cardiac muscle of diabetic mice
title_full_unstemmed	Anti-inflammatory and angiogenic effects of exercise training in cardiac muscle of diabetic mice
title_sort	anti-inflammatory and angiogenic effects of exercise training in cardiac muscle of diabetic mice
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/ff2c801d0d0044f28f421113290efaf9
work_keys_str_mv	AT brodericktl antiinflammatoryandangiogeniceffectsofexercisetrainingincardiacmuscleofdiabeticmice AT sennottjm antiinflammatoryandangiogeniceffectsofexercisetrainingincardiacmuscleofdiabeticmice AT gutkowskaj antiinflammatoryandangiogeniceffectsofexercisetrainingincardiacmuscleofdiabeticmice AT jankowskim antiinflammatoryandangiogeniceffectsofexercisetrainingincardiacmuscleofdiabeticmice
_version_	1718401833878159360

Anti-inflammatory and angiogenic effects of exercise training in cardiac muscle of diabetic mice

Ejemplares similares