Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity

Abstract Establishing correct neuronal cell identity is essential to build intricate neural tissue architecture and acquire precise neural function during vertebrate development. While it is known that transcription factors play important roles in retinal cell differentiation, the contribution of ep...

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Autores principales:	Shun Kubo, Haruka Yamamoto, Naoko Kajimura, Yoshihiro Omori, Yamato Maeda, Taro Chaya, Takahisa Furukawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff2fda7fd48f4509a217d5b5e5305247
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spelling	oai:doaj.org-article:ff2fda7fd48f4509a217d5b5e53052472021-12-02T14:21:43ZFunctional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity10.1038/s41598-021-83781-12045-2322https://doaj.org/article/ff2fda7fd48f4509a217d5b5e53052472021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83781-1https://doaj.org/toc/2045-2322Abstract Establishing correct neuronal cell identity is essential to build intricate neural tissue architecture and acquire precise neural function during vertebrate development. While it is known that transcription factors play important roles in retinal cell differentiation, the contribution of epigenetic factors to establishing cell identity during retinal development remains unclear. We previously reported that Samd7, a rod photoreceptor cell-specific sterile alpha motif (SAM) domain protein, functions as a Polycomb repressive complex 1 component (PRC1) that is essential for establishing rod identity. In the current study, we analyzed a functional role of Samd11, another photoreceptor-enriched SAM-domain protein, in photoreceptor differentiation and maturation. We observed that Samd11 interacts with Phc2 and Samd7, suggesting that Samd11 is a component of PRC1 in photoreceptor cells. We generated Samd11-null allele and established Samd7/11 double knock-out (DKO) mouse. The Samd7/11 DKO retina exhibits shortened photoreceptor outer segments by electron microscopy analysis. Microarray analysis revealed that Samd7/11 DKO up-regulated more retinal genes than Samd7 −/− alone, partial functional redundancy of Samd7 and Samd11. Taken together, the current results suggest that Samd7 and Samd11 are PRC1 components and that Samd7 is the major regulator while Samd11 is an accessory factor used for the establishment of precise rod photoreceptor identity.Shun KuboHaruka YamamotoNaoko KajimuraYoshihiro OmoriYamato MaedaTaro ChayaTakahisa FurukawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shun Kubo Haruka Yamamoto Naoko Kajimura Yoshihiro Omori Yamato Maeda Taro Chaya Takahisa Furukawa Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity
description	Abstract Establishing correct neuronal cell identity is essential to build intricate neural tissue architecture and acquire precise neural function during vertebrate development. While it is known that transcription factors play important roles in retinal cell differentiation, the contribution of epigenetic factors to establishing cell identity during retinal development remains unclear. We previously reported that Samd7, a rod photoreceptor cell-specific sterile alpha motif (SAM) domain protein, functions as a Polycomb repressive complex 1 component (PRC1) that is essential for establishing rod identity. In the current study, we analyzed a functional role of Samd11, another photoreceptor-enriched SAM-domain protein, in photoreceptor differentiation and maturation. We observed that Samd11 interacts with Phc2 and Samd7, suggesting that Samd11 is a component of PRC1 in photoreceptor cells. We generated Samd11-null allele and established Samd7/11 double knock-out (DKO) mouse. The Samd7/11 DKO retina exhibits shortened photoreceptor outer segments by electron microscopy analysis. Microarray analysis revealed that Samd7/11 DKO up-regulated more retinal genes than Samd7 −/− alone, partial functional redundancy of Samd7 and Samd11. Taken together, the current results suggest that Samd7 and Samd11 are PRC1 components and that Samd7 is the major regulator while Samd11 is an accessory factor used for the establishment of precise rod photoreceptor identity.
format	article
author	Shun Kubo Haruka Yamamoto Naoko Kajimura Yoshihiro Omori Yamato Maeda Taro Chaya Takahisa Furukawa
author_facet	Shun Kubo Haruka Yamamoto Naoko Kajimura Yoshihiro Omori Yamato Maeda Taro Chaya Takahisa Furukawa
author_sort	Shun Kubo
title	Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity
title_short	Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity
title_full	Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity
title_fullStr	Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity
title_full_unstemmed	Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity
title_sort	functional analysis of samd11, a retinal photoreceptor prc1 component, in establishing rod photoreceptor identity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ff2fda7fd48f4509a217d5b5e5305247
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Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity

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