Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system

Liting Guo,1 Haijun Zhang,1 Fei Wang,1 Ping Liu,1 Yonglu Wang,1,2 Guohua Xia,1 Ran Liu,1 Xueming Li,2 Haixiang Yin,2 Hulin Jiang,3 Baoan Chen11Department of Hematology and Oncology (Key Department of Jiangsu Medicine), The Affiliated Zhongda Hospital, Medical School of Southeast University, 2School...

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Autores principales:	Guo L, Zhang H, Wang F, Liu P, Wang Y, Xia G, Liu R, Li X, Yin H, Jiang H, Chen B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ff3591fe5f29496b866162b26a104de4
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spelling	oai:doaj.org-article:ff3591fe5f29496b866162b26a104de42021-12-02T06:47:06ZTargeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system1178-2013https://doaj.org/article/ff3591fe5f29496b866162b26a104de42015-07-01T00:00:00Zhttp://www.dovepress.com/targeted-multidrug-resistance-reversal-in-tumor-based-on-peg-pll-plga--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Liting Guo,1 Haijun Zhang,1 Fei Wang,1 Ping Liu,1 Yonglu Wang,1,2 Guohua Xia,1 Ran Liu,1 Xueming Li,2 Haixiang Yin,2 Hulin Jiang,3 Baoan Chen11Department of Hematology and Oncology (Key Department of Jiangsu Medicine), The Affiliated Zhongda Hospital, Medical School of Southeast University, 2School of Pharmacy, Nanjing University of Technology, 3Department of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, People’s Republic of ChinaAbstract: The study investigated the reversal of multidrug resistance (MDR) and the biodistribution of nanoparticles (NPs) that target leukemia cells in a nude mice model via a surface-bound transferrin (Tf). The cytotoxic cargo of daunorubicin (DNR) and tetrandrine (Tet) was protected in the NPs by an outer coat composed of polyethylene glycol (PEG)-poly-l-lysine (PLL)-poly(lactic-co-glycolic acid) (PLGA) NPs. Injection of DNR-Tet-Tf-PEG-PLL-PLGA NPs into nude mice bearing MDR leukemia cell K562/A02 xenografts was shown to inhibit tumor growth, and contemporaneous immunohistochemical analysis of tumor tissue showed the targeted NPs induced apoptosis in tumor cells. Targeted tumor cells exhibited a marked increase in Tf receptor expression, with noticeable decreases in P-glycoprotein, MDR protein, and nuclear factor κB, as assessed by quantitative real-time polymerase chain reaction and Western blot analysis. Moreover, the concentration of DNR was shown to increase in plasma, tumor tissue, and major organs. Flow cytometry analysis with a near-infrared fluorescent (NIRF) dye, NIR797, was used to study the effectiveness of Tf as a targeting group for leukemia cells, a finding that was supported by NIRF imaging in tumor-bearing nude mice. In summary, our studies show that DNR-Tet-Tf-PEG-PLL-PLGA NPs provide a specific and effective means to target cytotoxic drugs to MDR tumor cells.Keywords: PEG-PLL-PLGA nanoparticles, transferrin, tetrandrine, multidrug resistanceGuo LZhang HWang FLiu PWang YXia GLiu RLi XYin HJiang HChen BDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 4535-4547 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Guo L Zhang H Wang F Liu P Wang Y Xia G Liu R Li X Yin H Jiang H Chen B Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system
description	Liting Guo,1 Haijun Zhang,1 Fei Wang,1 Ping Liu,1 Yonglu Wang,1,2 Guohua Xia,1 Ran Liu,1 Xueming Li,2 Haixiang Yin,2 Hulin Jiang,3 Baoan Chen11Department of Hematology and Oncology (Key Department of Jiangsu Medicine), The Affiliated Zhongda Hospital, Medical School of Southeast University, 2School of Pharmacy, Nanjing University of Technology, 3Department of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, People’s Republic of ChinaAbstract: The study investigated the reversal of multidrug resistance (MDR) and the biodistribution of nanoparticles (NPs) that target leukemia cells in a nude mice model via a surface-bound transferrin (Tf). The cytotoxic cargo of daunorubicin (DNR) and tetrandrine (Tet) was protected in the NPs by an outer coat composed of polyethylene glycol (PEG)-poly-l-lysine (PLL)-poly(lactic-co-glycolic acid) (PLGA) NPs. Injection of DNR-Tet-Tf-PEG-PLL-PLGA NPs into nude mice bearing MDR leukemia cell K562/A02 xenografts was shown to inhibit tumor growth, and contemporaneous immunohistochemical analysis of tumor tissue showed the targeted NPs induced apoptosis in tumor cells. Targeted tumor cells exhibited a marked increase in Tf receptor expression, with noticeable decreases in P-glycoprotein, MDR protein, and nuclear factor κB, as assessed by quantitative real-time polymerase chain reaction and Western blot analysis. Moreover, the concentration of DNR was shown to increase in plasma, tumor tissue, and major organs. Flow cytometry analysis with a near-infrared fluorescent (NIRF) dye, NIR797, was used to study the effectiveness of Tf as a targeting group for leukemia cells, a finding that was supported by NIRF imaging in tumor-bearing nude mice. In summary, our studies show that DNR-Tet-Tf-PEG-PLL-PLGA NPs provide a specific and effective means to target cytotoxic drugs to MDR tumor cells.Keywords: PEG-PLL-PLGA nanoparticles, transferrin, tetrandrine, multidrug resistance
format	article
author	Guo L Zhang H Wang F Liu P Wang Y Xia G Liu R Li X Yin H Jiang H Chen B
author_facet	Guo L Zhang H Wang F Liu P Wang Y Xia G Liu R Li X Yin H Jiang H Chen B
author_sort	Guo L
title	Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system
title_short	Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system
title_full	Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system
title_fullStr	Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system
title_full_unstemmed	Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system
title_sort	targeted multidrug-resistance reversal in tumor based on peg-pll-plga polymer nano drug delivery system
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/ff3591fe5f29496b866162b26a104de4
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Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system

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