Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila

Abstract In Drosophila, three types of UAS vectors (UASt, UASp, and UASz) are currently available for use with the Gal4-UAS system. They have been used successfully in somatic cells and germline cells from ovaries. However, it remains unclear whether they are functional in the germline cells of embr...

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Autores principales:	Masaki Masukawa, Yuki Ishizaki, Hiroki Miura, Makoto Hayashi, Ryoma Ota, Satoru Kobayashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff3849d6ef974f47a26512af1fb87610
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spelling	oai:doaj.org-article:ff3849d6ef974f47a26512af1fb876102021-11-08T10:53:15ZMale-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila10.1038/s41598-021-00729-12045-2322https://doaj.org/article/ff3849d6ef974f47a26512af1fb876102021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00729-1https://doaj.org/toc/2045-2322Abstract In Drosophila, three types of UAS vectors (UASt, UASp, and UASz) are currently available for use with the Gal4-UAS system. They have been used successfully in somatic cells and germline cells from ovaries. However, it remains unclear whether they are functional in the germline cells of embryos, larvae, and adult testes. In this study, we found that all three types of UAS vectors were functional in the germline cells of embryos and larvae and that the UASt and UASz vectors were active in the germline of the distal tip region in adult testes. Moreover, we observed that protein expression from the UAS vectors was male-biased in germline cells of late embryos, whereas their respective mRNA expression levels were not. Furthermore, O-propargyl-puromycin (OPP) staining revealed that protein synthesis was male-biased in these germline cells. In addition, GO terms related to translation and ribosomal maturation were significantly enriched in the male germline. These observations show that translational activity is higher in male than in female germline cells. Therefore, we propose that male-biased protein synthesis may be responsible for the sex differences observed in the early germline.Masaki MasukawaYuki IshizakiHiroki MiuraMakoto HayashiRyoma OtaSatoru KobayashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masaki Masukawa Yuki Ishizaki Hiroki Miura Makoto Hayashi Ryoma Ota Satoru Kobayashi Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila
description	Abstract In Drosophila, three types of UAS vectors (UASt, UASp, and UASz) are currently available for use with the Gal4-UAS system. They have been used successfully in somatic cells and germline cells from ovaries. However, it remains unclear whether they are functional in the germline cells of embryos, larvae, and adult testes. In this study, we found that all three types of UAS vectors were functional in the germline cells of embryos and larvae and that the UASt and UASz vectors were active in the germline of the distal tip region in adult testes. Moreover, we observed that protein expression from the UAS vectors was male-biased in germline cells of late embryos, whereas their respective mRNA expression levels were not. Furthermore, O-propargyl-puromycin (OPP) staining revealed that protein synthesis was male-biased in these germline cells. In addition, GO terms related to translation and ribosomal maturation were significantly enriched in the male germline. These observations show that translational activity is higher in male than in female germline cells. Therefore, we propose that male-biased protein synthesis may be responsible for the sex differences observed in the early germline.
format	article
author	Masaki Masukawa Yuki Ishizaki Hiroki Miura Makoto Hayashi Ryoma Ota Satoru Kobayashi
author_facet	Masaki Masukawa Yuki Ishizaki Hiroki Miura Makoto Hayashi Ryoma Ota Satoru Kobayashi
author_sort	Masaki Masukawa
title	Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila
title_short	Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila
title_full	Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila
title_fullStr	Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila
title_full_unstemmed	Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila
title_sort	male-biased protein expression in primordial germ cells, identified through a comparative study of uas vectors in drosophila
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ff3849d6ef974f47a26512af1fb87610
work_keys_str_mv	AT masakimasukawa malebiasedproteinexpressioninprimordialgermcellsidentifiedthroughacomparativestudyofuasvectorsindrosophila AT yukiishizaki malebiasedproteinexpressioninprimordialgermcellsidentifiedthroughacomparativestudyofuasvectorsindrosophila AT hirokimiura malebiasedproteinexpressioninprimordialgermcellsidentifiedthroughacomparativestudyofuasvectorsindrosophila AT makotohayashi malebiasedproteinexpressioninprimordialgermcellsidentifiedthroughacomparativestudyofuasvectorsindrosophila AT ryomaota malebiasedproteinexpressioninprimordialgermcellsidentifiedthroughacomparativestudyofuasvectorsindrosophila AT satorukobayashi malebiasedproteinexpressioninprimordialgermcellsidentifiedthroughacomparativestudyofuasvectorsindrosophila
_version_	1718442582304882688

Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila

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