Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds

Abstract This study investigated the efficacy of Omega-7 isolated from the sea buckthorn oil (Polyvit Co., Ltd, Gangar Holding, Ulaanbaatar, Mongolia) in ovine burn wound healing models. In vitro, proliferation (colony-forming rate) and migration (scratch) assays using cultured primary ovine keratin...

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Autores principales:	Yosuke Niimi, Dannelys Pérez-Bello, Koji Ihara, Satoshi Fukuda, Sam Jacob, Clark R. Andersen, Tuvshintugs Baljinnyam, Jisoo Kim, Suzan Alharbi, Donald S. Prough, Perenlei Enkhbaatar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff39f71cdc104a4c97eb231c4c1d4253
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spelling	oai:doaj.org-article:ff39f71cdc104a4c97eb231c4c1d42532021-12-02T15:23:01ZOmega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds10.1038/s41598-020-79597-02045-2322https://doaj.org/article/ff39f71cdc104a4c97eb231c4c1d42532021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79597-0https://doaj.org/toc/2045-2322Abstract This study investigated the efficacy of Omega-7 isolated from the sea buckthorn oil (Polyvit Co., Ltd, Gangar Holding, Ulaanbaatar, Mongolia) in ovine burn wound healing models. In vitro, proliferation (colony-forming rate) and migration (scratch) assays using cultured primary ovine keratinocytes were performed with or without 0.025% and 0.08% Omega-7, respectively. The colony-forming rate of keratinocytes in the Omega-7 group at 72 and 96 h were significantly higher than in the control (P < 0.05). The percentage of closure in scratch assay in the Omega-7 group was significantly higher than in the control at 17 h (P < 0.05). In vivo, efficacy of 4% Omega-7 isolated from buckthorn oil was assessed at 7 and 14 days in grafted ovine burn and donor site wounds. Telomerase activity, keratinocyte growth factor, and wound nitrotyrosine levels were measured at day 14. Grafted sites: Un-epithelialized raw surface area was significantly lower and blood flow was significantly higher in the Omega-7-treated sites than in control sites at 7 and 14 days (P < 0.05). Telomerase activity and levels of keratinocyte growth factors were significantly higher in the Omega-7-treated sites after 14 days compared to those of control (P < 0.05). The wound 3-nitrotyrosine levels were significantly reduced by Omega-7. Donor sites: the complete epithelialization time was significantly shorter and blood flow at day 7 was significantly higher in the Omega-7-treated sites compared to control sites (P < 0.05). In summary, topical application of Omega-7 accelerates healing of both grafted burn and donor site wounds. Omega-7 should be considered as a cost-efficient and effective supplement therapy for burn wound healing.Yosuke NiimiDannelys Pérez-BelloKoji IharaSatoshi FukudaSam JacobClark R. AndersenTuvshintugs BaljinnyamJisoo KimSuzan AlharbiDonald S. ProughPerenlei EnkhbaatarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yosuke Niimi Dannelys Pérez-Bello Koji Ihara Satoshi Fukuda Sam Jacob Clark R. Andersen Tuvshintugs Baljinnyam Jisoo Kim Suzan Alharbi Donald S. Prough Perenlei Enkhbaatar Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds
description	Abstract This study investigated the efficacy of Omega-7 isolated from the sea buckthorn oil (Polyvit Co., Ltd, Gangar Holding, Ulaanbaatar, Mongolia) in ovine burn wound healing models. In vitro, proliferation (colony-forming rate) and migration (scratch) assays using cultured primary ovine keratinocytes were performed with or without 0.025% and 0.08% Omega-7, respectively. The colony-forming rate of keratinocytes in the Omega-7 group at 72 and 96 h were significantly higher than in the control (P < 0.05). The percentage of closure in scratch assay in the Omega-7 group was significantly higher than in the control at 17 h (P < 0.05). In vivo, efficacy of 4% Omega-7 isolated from buckthorn oil was assessed at 7 and 14 days in grafted ovine burn and donor site wounds. Telomerase activity, keratinocyte growth factor, and wound nitrotyrosine levels were measured at day 14. Grafted sites: Un-epithelialized raw surface area was significantly lower and blood flow was significantly higher in the Omega-7-treated sites than in control sites at 7 and 14 days (P < 0.05). Telomerase activity and levels of keratinocyte growth factors were significantly higher in the Omega-7-treated sites after 14 days compared to those of control (P < 0.05). The wound 3-nitrotyrosine levels were significantly reduced by Omega-7. Donor sites: the complete epithelialization time was significantly shorter and blood flow at day 7 was significantly higher in the Omega-7-treated sites compared to control sites (P < 0.05). In summary, topical application of Omega-7 accelerates healing of both grafted burn and donor site wounds. Omega-7 should be considered as a cost-efficient and effective supplement therapy for burn wound healing.
format	article
author	Yosuke Niimi Dannelys Pérez-Bello Koji Ihara Satoshi Fukuda Sam Jacob Clark R. Andersen Tuvshintugs Baljinnyam Jisoo Kim Suzan Alharbi Donald S. Prough Perenlei Enkhbaatar
author_facet	Yosuke Niimi Dannelys Pérez-Bello Koji Ihara Satoshi Fukuda Sam Jacob Clark R. Andersen Tuvshintugs Baljinnyam Jisoo Kim Suzan Alharbi Donald S. Prough Perenlei Enkhbaatar
author_sort	Yosuke Niimi
title	Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds
title_short	Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds
title_full	Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds
title_fullStr	Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds
title_full_unstemmed	Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds
title_sort	omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ff39f71cdc104a4c97eb231c4c1d4253
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Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds

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