Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport.

The Golgi apparatus in mammalian cells is composed of flattened cisternae that are densely packed to form stacks. We have used the Golgi stacking protein GRASP65 as a tool to modify the stacking state of Golgi cisternae. We established an assay to measure protein transport to the cell surface in pos...

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Autores principales:	Yanzhuang Wang, Jen-Hsuan Wei, Blaine Bisel, Danming Tang, Joachim Seemann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff3c1a310827410e8a8804e22c7bd2be
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id	oai:doaj.org-article:ff3c1a310827410e8a8804e22c7bd2be
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spelling	oai:doaj.org-article:ff3c1a310827410e8a8804e22c7bd2be2021-11-25T06:13:19ZGolgi cisternal unstacking stimulates COPI vesicle budding and protein transport.1932-620310.1371/journal.pone.0001647https://doaj.org/article/ff3c1a310827410e8a8804e22c7bd2be2008-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18297130/?tool=EBIhttps://doaj.org/toc/1932-6203The Golgi apparatus in mammalian cells is composed of flattened cisternae that are densely packed to form stacks. We have used the Golgi stacking protein GRASP65 as a tool to modify the stacking state of Golgi cisternae. We established an assay to measure protein transport to the cell surface in post-mitotic cells in which the Golgi was unstacked. Cells with an unstacked Golgi showed a higher transport rate compared to cells with stacked Golgi membranes. Vesicle budding from unstacked cisternae in vitro was significantly increased compared to stacked membranes. These results suggest that Golgi cisternal stacking can directly regulate vesicle formation and thus the rate of protein transport through the Golgi. The results further suggest that at the onset of mitosis, unstacking of cisternae allows extensive and rapid vesiculation of the Golgi in preparation for its subsequent partitioning.Yanzhuang WangJen-Hsuan WeiBlaine BiselDanming TangJoachim SeemannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 2, p e1647 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanzhuang Wang Jen-Hsuan Wei Blaine Bisel Danming Tang Joachim Seemann Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport.
description	The Golgi apparatus in mammalian cells is composed of flattened cisternae that are densely packed to form stacks. We have used the Golgi stacking protein GRASP65 as a tool to modify the stacking state of Golgi cisternae. We established an assay to measure protein transport to the cell surface in post-mitotic cells in which the Golgi was unstacked. Cells with an unstacked Golgi showed a higher transport rate compared to cells with stacked Golgi membranes. Vesicle budding from unstacked cisternae in vitro was significantly increased compared to stacked membranes. These results suggest that Golgi cisternal stacking can directly regulate vesicle formation and thus the rate of protein transport through the Golgi. The results further suggest that at the onset of mitosis, unstacking of cisternae allows extensive and rapid vesiculation of the Golgi in preparation for its subsequent partitioning.
format	article
author	Yanzhuang Wang Jen-Hsuan Wei Blaine Bisel Danming Tang Joachim Seemann
author_facet	Yanzhuang Wang Jen-Hsuan Wei Blaine Bisel Danming Tang Joachim Seemann
author_sort	Yanzhuang Wang
title	Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport.
title_short	Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport.
title_full	Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport.
title_fullStr	Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport.
title_full_unstemmed	Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport.
title_sort	golgi cisternal unstacking stimulates copi vesicle budding and protein transport.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/ff3c1a310827410e8a8804e22c7bd2be
work_keys_str_mv	AT yanzhuangwang golgicisternalunstackingstimulatescopivesiclebuddingandproteintransport AT jenhsuanwei golgicisternalunstackingstimulatescopivesiclebuddingandproteintransport AT blainebisel golgicisternalunstackingstimulatescopivesiclebuddingandproteintransport AT danmingtang golgicisternalunstackingstimulatescopivesiclebuddingandproteintransport AT joachimseemann golgicisternalunstackingstimulatescopivesiclebuddingandproteintransport
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Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport.

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