The Relationship of <i>ABCB1/MDR1</i> and <i>CYP1A1</i> Variants with the Risk of Disease Development and Shortening of Overall Survival in Patients with Multiple Myeloma

The Relationship of <i>ABCB1/MDR1</i> and <i>CYP1A1</i> Variants with the Risk of Disease Development and Shortening of Overall Survival in Patients with Multiple Myeloma

(1) Background: The aim of our study was to analyze the possible relationship of <i>ABCB1</i> and <i>CYP1A1</i> gene variants with susceptibility and outcome of multiple myeloma (MM); (2) Methods: Genomic DNA samples from 110 newly-diagnosed MM patients and 100 healthy blood...

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Autores principales: Szymon Zmorzynski, Magdalena Wojcierowska-Litwin, Sylwia Popek-Marciniec, Aneta Szudy-Szczyrek, Wojciech Styk, Sylwia Chocholska, Agata Anna Filip
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
multiple myeloma
genetic variants
polymorphisms
<i>ABCB1</i> gene
<i>CYP1A1</i> gene
Medicine
R
Acceso en línea:https://doaj.org/article/ff4214997cc445b4b826f33c0c5a2080
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spelling oai:doaj.org-article:ff4214997cc445b4b826f33c0c5a20802021-11-25T18:01:14ZThe Relationship of <i>ABCB1/MDR1</i> and <i>CYP1A1</i> Variants with the Risk of Disease Development and Shortening of Overall Survival in Patients with Multiple Myeloma10.3390/jcm102252762077-0383https://doaj.org/article/ff4214997cc445b4b826f33c0c5a20802021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/22/5276https://doaj.org/toc/2077-0383(1) Background: The aim of our study was to analyze the possible relationship of <i>ABCB1</i> and <i>CYP1A1</i> gene variants with susceptibility and outcome of multiple myeloma (MM); (2) Methods: Genomic DNA samples from 110 newly-diagnosed MM patients and 100 healthy blood donors were analyzed by methods-PCR-RFLP (for <i>ABCB1</i> 3435C > T, <i>CYP1A1</i> 6235T > C—m1), automated DNA sequencing (for <i>ABCB1</i> 1236C > T, 2677G > T/A) and allele-specific PCR (for <i>CYP1A1</i> 4889A > G—m2); (3) Results: The genotypic frequencies of <i>CYP1A1</i> 4889A > G variant were not in Hardy-Weinberg equilibrium for MM patients. The presence of m1 and m2 <i>CYP1A1</i> alleles decreased the risk of MM—OR = 0.49 (<i>p</i> = 0.011) and OR = 0.27 (<i>p</i> = 0.0003), respectively. In turn, TT genotype (<i>ABCB1</i> 2677G > T/A) increased the risk of this disease (<i>p</i> = 0.007). In the multivariate Cox analysis CT + TT genotypes (<i>ABCB1</i> 3435C > T) were associated with decreased risk of death (HR = 0.29, <i>p</i> = 0.04). In log-rank test in patients with CT genotype (<i>ABCB1</i> 3435C > T) was observed association of overall survival with the type of treatment; (4) Conclusions: Our findings suggest that T-alleles of <i>ABCB1</i> 2677G > T/A and m1/m2 alleles of <i>CYP1A1</i> affected the susceptibility of MM. Moreover, T-allele of <i>ABCB1</i> 3435C > T might be independent positive prognostic factor in MM.Szymon ZmorzynskiMagdalena Wojcierowska-LitwinSylwia Popek-MarciniecAneta Szudy-SzczyrekWojciech StykSylwia ChocholskaAgata Anna FilipMDPI AGarticlemultiple myelomagenetic variantspolymorphisms<i>ABCB1</i> gene<i>CYP1A1</i> geneMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5276, p 5276 (2021)
institution DOAJ
collection DOAJ
language EN
topic multiple myeloma
genetic variants
polymorphisms
<i>ABCB1</i> gene
<i>CYP1A1</i> gene
Medicine
R
spellingShingle multiple myeloma
genetic variants
polymorphisms
<i>ABCB1</i> gene
<i>CYP1A1</i> gene
Medicine
R
Szymon Zmorzynski
Magdalena Wojcierowska-Litwin
Sylwia Popek-Marciniec
Aneta Szudy-Szczyrek
Wojciech Styk
Sylwia Chocholska
Agata Anna Filip
The Relationship of <i>ABCB1/MDR1</i> and <i>CYP1A1</i> Variants with the Risk of Disease Development and Shortening of Overall Survival in Patients with Multiple Myeloma
description (1) Background: The aim of our study was to analyze the possible relationship of <i>ABCB1</i> and <i>CYP1A1</i> gene variants with susceptibility and outcome of multiple myeloma (MM); (2) Methods: Genomic DNA samples from 110 newly-diagnosed MM patients and 100 healthy blood donors were analyzed by methods-PCR-RFLP (for <i>ABCB1</i> 3435C > T, <i>CYP1A1</i> 6235T > C—m1), automated DNA sequencing (for <i>ABCB1</i> 1236C > T, 2677G > T/A) and allele-specific PCR (for <i>CYP1A1</i> 4889A > G—m2); (3) Results: The genotypic frequencies of <i>CYP1A1</i> 4889A > G variant were not in Hardy-Weinberg equilibrium for MM patients. The presence of m1 and m2 <i>CYP1A1</i> alleles decreased the risk of MM—OR = 0.49 (<i>p</i> = 0.011) and OR = 0.27 (<i>p</i> = 0.0003), respectively. In turn, TT genotype (<i>ABCB1</i> 2677G > T/A) increased the risk of this disease (<i>p</i> = 0.007). In the multivariate Cox analysis CT + TT genotypes (<i>ABCB1</i> 3435C > T) were associated with decreased risk of death (HR = 0.29, <i>p</i> = 0.04). In log-rank test in patients with CT genotype (<i>ABCB1</i> 3435C > T) was observed association of overall survival with the type of treatment; (4) Conclusions: Our findings suggest that T-alleles of <i>ABCB1</i> 2677G > T/A and m1/m2 alleles of <i>CYP1A1</i> affected the susceptibility of MM. Moreover, T-allele of <i>ABCB1</i> 3435C > T might be independent positive prognostic factor in MM.
format article
author Szymon Zmorzynski
Magdalena Wojcierowska-Litwin
Sylwia Popek-Marciniec
Aneta Szudy-Szczyrek
Wojciech Styk
Sylwia Chocholska
Agata Anna Filip
author_facet Szymon Zmorzynski
Magdalena Wojcierowska-Litwin
Sylwia Popek-Marciniec
Aneta Szudy-Szczyrek
Wojciech Styk
Sylwia Chocholska
Agata Anna Filip
author_sort Szymon Zmorzynski
title The Relationship of <i>ABCB1/MDR1</i> and <i>CYP1A1</i> Variants with the Risk of Disease Development and Shortening of Overall Survival in Patients with Multiple Myeloma
title_short The Relationship of <i>ABCB1/MDR1</i> and <i>CYP1A1</i> Variants with the Risk of Disease Development and Shortening of Overall Survival in Patients with Multiple Myeloma
title_full The Relationship of <i>ABCB1/MDR1</i> and <i>CYP1A1</i> Variants with the Risk of Disease Development and Shortening of Overall Survival in Patients with Multiple Myeloma
title_fullStr The Relationship of <i>ABCB1/MDR1</i> and <i>CYP1A1</i> Variants with the Risk of Disease Development and Shortening of Overall Survival in Patients with Multiple Myeloma
title_full_unstemmed The Relationship of <i>ABCB1/MDR1</i> and <i>CYP1A1</i> Variants with the Risk of Disease Development and Shortening of Overall Survival in Patients with Multiple Myeloma
title_sort relationship of <i>abcb1/mdr1</i> and <i>cyp1a1</i> variants with the risk of disease development and shortening of overall survival in patients with multiple myeloma
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/ff4214997cc445b4b826f33c0c5a2080
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