Complex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes

Abstract Analysis of spider venom gland transcriptomes focuses on the identification of possible neurotoxins, proteins and enzymes. Here, the first comprehensive transcriptome analysis of cupiennins, small linear cationic peptides, also known as cytolytic or antimicrobial peptides, is reported from...

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Autor principal:	Lucia Kuhn-Nentwig
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff4889b0b2a346b69b04099546cded41
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spelling	oai:doaj.org-article:ff4889b0b2a346b69b04099546cded412021-12-02T14:21:43ZComplex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes10.1038/s41598-021-83624-z2045-2322https://doaj.org/article/ff4889b0b2a346b69b04099546cded412021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83624-zhttps://doaj.org/toc/2045-2322Abstract Analysis of spider venom gland transcriptomes focuses on the identification of possible neurotoxins, proteins and enzymes. Here, the first comprehensive transcriptome analysis of cupiennins, small linear cationic peptides, also known as cytolytic or antimicrobial peptides, is reported from the venom gland transcriptome of Cupiennius salei by 454- and Illumina 3000 sequencing. Four transcript families with complex precursor structures are responsible for the expression of 179 linear peptides. Within the transcript families, after an anionic propeptide, cationic linear peptides are separated by anionic linkers, which are transcript family specific. The C-terminus of the transcript families is characterized by a linear peptide or truncated linkers with unknown function. A new identified posttranslational processing mechanism explains the presence of the two-chain CsTx-16 family in the venom. The high diversity of linear peptides in the venom of a spider and this unique synthesis process is at least genus specific as verified with Cupiennius getazi.Lucia Kuhn-NentwigNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucia Kuhn-Nentwig Complex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes
description	Abstract Analysis of spider venom gland transcriptomes focuses on the identification of possible neurotoxins, proteins and enzymes. Here, the first comprehensive transcriptome analysis of cupiennins, small linear cationic peptides, also known as cytolytic or antimicrobial peptides, is reported from the venom gland transcriptome of Cupiennius salei by 454- and Illumina 3000 sequencing. Four transcript families with complex precursor structures are responsible for the expression of 179 linear peptides. Within the transcript families, after an anionic propeptide, cationic linear peptides are separated by anionic linkers, which are transcript family specific. The C-terminus of the transcript families is characterized by a linear peptide or truncated linkers with unknown function. A new identified posttranslational processing mechanism explains the presence of the two-chain CsTx-16 family in the venom. The high diversity of linear peptides in the venom of a spider and this unique synthesis process is at least genus specific as verified with Cupiennius getazi.
format	article
author	Lucia Kuhn-Nentwig
author_facet	Lucia Kuhn-Nentwig
author_sort	Lucia Kuhn-Nentwig
title	Complex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes
title_short	Complex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes
title_full	Complex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes
title_fullStr	Complex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes
title_full_unstemmed	Complex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes
title_sort	complex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ff4889b0b2a346b69b04099546cded41
work_keys_str_mv	AT luciakuhnnentwig complexprecursorstructuresofcytolyticcupienninsidentifiedinspidervenomglandtranscriptomes
_version_	1718391526477791232

Complex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes

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