Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations

SCN5A gene loss-of-function mutations are commonly associated with Brugada syndrome, which represents a risk of lethal arrhythmias and sudden cardiac death. The present report describes the generation of two human induced pluripotent stem cell (iPSC) lines reprogrammed from two Brugada syndrome affe...

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Autores principales:	Nadjet Belbachir, Celine Lai, June-Wha Rhee, Yan Zhuge, Marco V. Perez, Karim Sallam, Joseph C. Wu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ff538844cddb40a2a2540fd2b35f159d
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spelling	oai:doaj.org-article:ff538844cddb40a2a2540fd2b35f159d2021-12-02T05:00:18ZGeneration of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations1873-506110.1016/j.scr.2021.102605https://doaj.org/article/ff538844cddb40a2a2540fd2b35f159d2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1873506121004529https://doaj.org/toc/1873-5061SCN5A gene loss-of-function mutations are commonly associated with Brugada syndrome, which represents a risk of lethal arrhythmias and sudden cardiac death. The present report describes the generation of two human induced pluripotent stem cell (iPSC) lines reprogrammed from two Brugada syndrome affected patients carrying SCN5A mutations, c.53506 G>A and c.2102 C>T, respectively. Pluripotency markers, karyotype stability, and differentiation capability into derivatives of the three germ layers were assessed and described in the present report. These lines can be used as a reliable cell model for Brugada syndrome investigations and characterization of leading cellular mechanisms.Nadjet BelbachirCeline LaiJune-Wha RheeYan ZhugeMarco V. PerezKarim SallamJoseph C. WuElsevierarticleBiology (General)QH301-705.5ENStem Cell Research, Vol 57, Iss , Pp 102605- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Nadjet Belbachir Celine Lai June-Wha Rhee Yan Zhuge Marco V. Perez Karim Sallam Joseph C. Wu Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations
description	SCN5A gene loss-of-function mutations are commonly associated with Brugada syndrome, which represents a risk of lethal arrhythmias and sudden cardiac death. The present report describes the generation of two human induced pluripotent stem cell (iPSC) lines reprogrammed from two Brugada syndrome affected patients carrying SCN5A mutations, c.53506 G>A and c.2102 C>T, respectively. Pluripotency markers, karyotype stability, and differentiation capability into derivatives of the three germ layers were assessed and described in the present report. These lines can be used as a reliable cell model for Brugada syndrome investigations and characterization of leading cellular mechanisms.
format	article
author	Nadjet Belbachir Celine Lai June-Wha Rhee Yan Zhuge Marco V. Perez Karim Sallam Joseph C. Wu
author_facet	Nadjet Belbachir Celine Lai June-Wha Rhee Yan Zhuge Marco V. Perez Karim Sallam Joseph C. Wu
author_sort	Nadjet Belbachir
title	Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations
title_short	Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations
title_full	Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations
title_fullStr	Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations
title_full_unstemmed	Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations
title_sort	generation of two induced pluripotent stem cell lines from brugada syndrome affected patients carrying scn5a mutations
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/ff538844cddb40a2a2540fd2b35f159d
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Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations

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