Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues

Human bone is a specialized tissue with unique material properties, providing mechanical support and resistance to the skeleton and simultaneously assuring capability of adaptation and remodelling. Knowing the properties of such a structure down to the micro-scale is of utmost importance, not only f...

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Autores principales:	Martina Alunni Cardinali, Assunta Morresi, Daniele Fioretto, Leonardo Vivarelli, Dante Dallari, Marco Govoni
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Raman spectroscopy Brillouin spectroscopy bone material characterization bone micro-scale architecture Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
Acceso en línea:	https://doaj.org/article/ff5b81f508b84f2187b55e63adff7857
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spelling	oai:doaj.org-article:ff5b81f508b84f2187b55e63adff78572021-11-25T18:14:17ZBrillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues10.3390/ma142268691996-1944https://doaj.org/article/ff5b81f508b84f2187b55e63adff78572021-11-01T00:00:00Zhttps://www.mdpi.com/1996-1944/14/22/6869https://doaj.org/toc/1996-1944Human bone is a specialized tissue with unique material properties, providing mechanical support and resistance to the skeleton and simultaneously assuring capability of adaptation and remodelling. Knowing the properties of such a structure down to the micro-scale is of utmost importance, not only for the design of effective biomimetic materials but also to be able to detect pathological alterations in material properties, such as micro-fractures or abnormal tissue remodelling. The Brillouin and Raman micro-spectroscopic (BRmS) approach has the potential to become a first-choice technique, as it is capable of simultaneously investigating samples’ mechanical and structural properties in a non-destructive and label-free way. Here, we perform a mapping of cortical and trabecular bone sections of a femoral epiphysis, demonstrating the capability of the technique for discovering the morpho-mechanics of cells, the extracellular matrix, and marrow constituents. Moreover, the interpretation of Brillouin and Raman spectra merged with an approach of data mining is used to compare the mechanical alterations in specimens excised from distinct anatomical areas and subjected to different sample processing. The results disclose in both cases specific alterations in the morphology and/or in the tissue chemical make-up, which strongly affects bone mechanical properties, providing a method potentially extendable to other important biomedical issues.Martina Alunni CardinaliAssunta MorresiDaniele FiorettoLeonardo VivarelliDante DallariMarco GovoniMDPI AGarticleRaman spectroscopyBrillouin spectroscopybone material characterizationbone micro-scale architectureTechnologyTElectrical engineering. Electronics. Nuclear engineeringTK1-9971Engineering (General). Civil engineering (General)TA1-2040MicroscopyQH201-278.5Descriptive and experimental mechanicsQC120-168.85ENMaterials, Vol 14, Iss 6869, p 6869 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Raman spectroscopy Brillouin spectroscopy bone material characterization bone micro-scale architecture Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
spellingShingle	Raman spectroscopy Brillouin spectroscopy bone material characterization bone micro-scale architecture Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Martina Alunni Cardinali Assunta Morresi Daniele Fioretto Leonardo Vivarelli Dante Dallari Marco Govoni Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues
description	Human bone is a specialized tissue with unique material properties, providing mechanical support and resistance to the skeleton and simultaneously assuring capability of adaptation and remodelling. Knowing the properties of such a structure down to the micro-scale is of utmost importance, not only for the design of effective biomimetic materials but also to be able to detect pathological alterations in material properties, such as micro-fractures or abnormal tissue remodelling. The Brillouin and Raman micro-spectroscopic (BRmS) approach has the potential to become a first-choice technique, as it is capable of simultaneously investigating samples’ mechanical and structural properties in a non-destructive and label-free way. Here, we perform a mapping of cortical and trabecular bone sections of a femoral epiphysis, demonstrating the capability of the technique for discovering the morpho-mechanics of cells, the extracellular matrix, and marrow constituents. Moreover, the interpretation of Brillouin and Raman spectra merged with an approach of data mining is used to compare the mechanical alterations in specimens excised from distinct anatomical areas and subjected to different sample processing. The results disclose in both cases specific alterations in the morphology and/or in the tissue chemical make-up, which strongly affects bone mechanical properties, providing a method potentially extendable to other important biomedical issues.
format	article
author	Martina Alunni Cardinali Assunta Morresi Daniele Fioretto Leonardo Vivarelli Dante Dallari Marco Govoni
author_facet	Martina Alunni Cardinali Assunta Morresi Daniele Fioretto Leonardo Vivarelli Dante Dallari Marco Govoni
author_sort	Martina Alunni Cardinali
title	Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues
title_short	Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues
title_full	Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues
title_fullStr	Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues
title_full_unstemmed	Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues
title_sort	brillouin and raman micro-spectroscopy: a tool for micro-mechanical and structural characterization of cortical and trabecular bone tissues
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ff5b81f508b84f2187b55e63adff7857
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Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues

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