Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle

Circular RNAs (circRNAs) are a newly discovered family of regulatory RNAs generated through backsplicing. Genome-wide profiling of circRNAs found that circRNAs are ubiquitously expressed and regulate gene expression by acting as a sponge for RNA-binding proteins (RBPs) and microRNAs (miRNAs). To ide...

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Autores principales:	Arundhati Das, Sharmishtha Shyamal, Tanvi Sinha, Smruti Sambhav Mishra, Amaresh C. Panda
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	circular RNA microRNA skeletal muscle myogenesis ceRNA Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ff7854e951704447a029bccf02496f88
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spelling	oai:doaj.org-article:ff7854e951704447a029bccf02496f882021-12-01T12:28:45ZIdentification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle2296-889X10.3389/fmolb.2021.762185https://doaj.org/article/ff7854e951704447a029bccf02496f882021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.762185/fullhttps://doaj.org/toc/2296-889XCircular RNAs (circRNAs) are a newly discovered family of regulatory RNAs generated through backsplicing. Genome-wide profiling of circRNAs found that circRNAs are ubiquitously expressed and regulate gene expression by acting as a sponge for RNA-binding proteins (RBPs) and microRNAs (miRNAs). To identify circRNAs expressed in mouse skeletal muscle, we performed high-throughput RNA-sequencing of circRNA-enriched gastrocnemius muscle RNA samples, which identified more than 1,200 circRNAs. In addition, we have identified more than 14,000 and 15,000 circRNAs in aging human skeletal muscle tissue and satellite cells, respectively. A subset of abundant circRNAs was analyzed by RT-PCR, Sanger sequencing, and RNase R digestion assays to validate their expression in mouse skeletal muscle tissues. Analysis of the circRNA-miRNA-mRNA regulatory network revealed that conserved circNfix might associate with miR-204-5p, a suppressor of myocyte enhancer factor 2c (Mef2c) expression. To support the hypothesis that circNfix might regulate myogenesis by controlling Mef2c expression, silencing circNfix moderately reduced Mef2c mRNA expression and inhibited C2C12 differentiation. We propose that circNfix promotes MEF2C expression during muscle cell differentiation in part by acting as a sponge for miR-204-5p.Arundhati DasArundhati DasSharmishtha ShyamalTanvi SinhaSmruti Sambhav MishraAmaresh C. PandaFrontiers Media S.A.articlecircular RNAmicroRNAskeletal musclemyogenesisceRNABiology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circular RNA microRNA skeletal muscle myogenesis ceRNA Biology (General) QH301-705.5
spellingShingle	circular RNA microRNA skeletal muscle myogenesis ceRNA Biology (General) QH301-705.5 Arundhati Das Arundhati Das Sharmishtha Shyamal Tanvi Sinha Smruti Sambhav Mishra Amaresh C. Panda Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
description	Circular RNAs (circRNAs) are a newly discovered family of regulatory RNAs generated through backsplicing. Genome-wide profiling of circRNAs found that circRNAs are ubiquitously expressed and regulate gene expression by acting as a sponge for RNA-binding proteins (RBPs) and microRNAs (miRNAs). To identify circRNAs expressed in mouse skeletal muscle, we performed high-throughput RNA-sequencing of circRNA-enriched gastrocnemius muscle RNA samples, which identified more than 1,200 circRNAs. In addition, we have identified more than 14,000 and 15,000 circRNAs in aging human skeletal muscle tissue and satellite cells, respectively. A subset of abundant circRNAs was analyzed by RT-PCR, Sanger sequencing, and RNase R digestion assays to validate their expression in mouse skeletal muscle tissues. Analysis of the circRNA-miRNA-mRNA regulatory network revealed that conserved circNfix might associate with miR-204-5p, a suppressor of myocyte enhancer factor 2c (Mef2c) expression. To support the hypothesis that circNfix might regulate myogenesis by controlling Mef2c expression, silencing circNfix moderately reduced Mef2c mRNA expression and inhibited C2C12 differentiation. We propose that circNfix promotes MEF2C expression during muscle cell differentiation in part by acting as a sponge for miR-204-5p.
format	article
author	Arundhati Das Arundhati Das Sharmishtha Shyamal Tanvi Sinha Smruti Sambhav Mishra Amaresh C. Panda
author_facet	Arundhati Das Arundhati Das Sharmishtha Shyamal Tanvi Sinha Smruti Sambhav Mishra Amaresh C. Panda
author_sort	Arundhati Das
title	Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title_short	Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title_full	Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title_fullStr	Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title_full_unstemmed	Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title_sort	identification of potential circrna-microrna-mrna regulatory network in skeletal muscle
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ff7854e951704447a029bccf02496f88
work_keys_str_mv	AT arundhatidas identificationofpotentialcircrnamicrornamrnaregulatorynetworkinskeletalmuscle AT arundhatidas identificationofpotentialcircrnamicrornamrnaregulatorynetworkinskeletalmuscle AT sharmishthashyamal identificationofpotentialcircrnamicrornamrnaregulatorynetworkinskeletalmuscle AT tanvisinha identificationofpotentialcircrnamicrornamrnaregulatorynetworkinskeletalmuscle AT smrutisambhavmishra identificationofpotentialcircrnamicrornamrnaregulatorynetworkinskeletalmuscle AT amareshcpanda identificationofpotentialcircrnamicrornamrnaregulatorynetworkinskeletalmuscle
_version_	1718405213704945664

Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle

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