A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

Targeted CRISPR libraries expand the use of genetic screens across experimental conditions. Here, the authors develop a method for generating and analysing small scale custom CRISPR libraries and use it in the human and livestock pathogen Toxoplasma gondii to identify virulence factors in mice.

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Detalles Bibliográficos
Autores principales: Joanna Young, Caia Dominicus, Jeanette Wagener, Simon Butterworth, Xingda Ye, Gavin Kelly, Merav Ordan, Becky Saunders, Rachael Instrell, Michael Howell, Aengus Stewart, Moritz Treeck
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Science
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Acceso en línea:https://doaj.org/article/ff7e1fbbf413468e9415fbefc93c42f1
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Sumario:Targeted CRISPR libraries expand the use of genetic screens across experimental conditions. Here, the authors develop a method for generating and analysing small scale custom CRISPR libraries and use it in the human and livestock pathogen Toxoplasma gondii to identify virulence factors in mice.

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