A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

Targeted CRISPR libraries expand the use of genetic screens across experimental conditions. Here, the authors develop a method for generating and analysing small scale custom CRISPR libraries and use it in the human and livestock pathogen Toxoplasma gondii to identify virulence factors in mice.

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Autores principales:	Joanna Young, Caia Dominicus, Jeanette Wagener, Simon Butterworth, Xingda Ye, Gavin Kelly, Merav Ordan, Becky Saunders, Rachael Instrell, Michael Howell, Aengus Stewart, Moritz Treeck
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ff7e1fbbf413468e9415fbefc93c42f1
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spelling	oai:doaj.org-article:ff7e1fbbf413468e9415fbefc93c42f12021-12-02T14:39:59ZA CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice10.1038/s41467-019-11855-w2041-1723https://doaj.org/article/ff7e1fbbf413468e9415fbefc93c42f12019-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11855-whttps://doaj.org/toc/2041-1723Targeted CRISPR libraries expand the use of genetic screens across experimental conditions. Here, the authors develop a method for generating and analysing small scale custom CRISPR libraries and use it in the human and livestock pathogen Toxoplasma gondii to identify virulence factors in mice.Joanna YoungCaia DominicusJeanette WagenerSimon ButterworthXingda YeGavin KellyMerav OrdanBecky SaundersRachael InstrellMichael HowellAengus StewartMoritz TreeckNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Joanna Young Caia Dominicus Jeanette Wagener Simon Butterworth Xingda Ye Gavin Kelly Merav Ordan Becky Saunders Rachael Instrell Michael Howell Aengus Stewart Moritz Treeck A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice
description	Targeted CRISPR libraries expand the use of genetic screens across experimental conditions. Here, the authors develop a method for generating and analysing small scale custom CRISPR libraries and use it in the human and livestock pathogen Toxoplasma gondii to identify virulence factors in mice.
format	article
author	Joanna Young Caia Dominicus Jeanette Wagener Simon Butterworth Xingda Ye Gavin Kelly Merav Ordan Becky Saunders Rachael Instrell Michael Howell Aengus Stewart Moritz Treeck
author_facet	Joanna Young Caia Dominicus Jeanette Wagener Simon Butterworth Xingda Ye Gavin Kelly Merav Ordan Becky Saunders Rachael Instrell Michael Howell Aengus Stewart Moritz Treeck
author_sort	Joanna Young
title	A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice
title_short	A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice
title_full	A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice
title_fullStr	A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice
title_full_unstemmed	A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice
title_sort	crispr platform for targeted in vivo screens identifies toxoplasma gondii virulence factors in mice
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ff7e1fbbf413468e9415fbefc93c42f1
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A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

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