Affibody Functionalized Beads for the Highly Sensitive Detection of Cancer Cell-Derived Exosomes

Exosomes belong to the class of extracellular vesicles of endocytic origin, which are regarded as a promising source of cancer biomarkers in liquid biopsy. As a result, an accurate, sensitive, and specific quantification of these nano-sized particles is of significant importance. Affinity-based appr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nima Sayyadi, Sareh Zhand, Sajad Razavi Bazaz, Majid Ebrahimi Warkiani
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Affibody cancer-derived exosome exosome biomarker Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/ff806c05a38440a49a04cbcc16fb1329
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ff806c05a38440a49a04cbcc16fb1329
record_format	dspace
spelling	oai:doaj.org-article:ff806c05a38440a49a04cbcc16fb13292021-11-11T17:25:40ZAffibody Functionalized Beads for the Highly Sensitive Detection of Cancer Cell-Derived Exosomes10.3390/ijms2221120141422-00671661-6596https://doaj.org/article/ff806c05a38440a49a04cbcc16fb13292021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12014https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Exosomes belong to the class of extracellular vesicles of endocytic origin, which are regarded as a promising source of cancer biomarkers in liquid biopsy. As a result, an accurate, sensitive, and specific quantification of these nano-sized particles is of significant importance. Affinity-based approaches are recognized as the most valuable technique for exosome isolation and characterization. Indeed, Affibody biomolecules are a type of protein scaffold engineered with small size and enjoy the features of high thermal stability, affinity, and specificity. While the utilization of antibodies, aptamers, and other biologically active substances for exosome detection has been reported widely, there are no reports describing Affibody molecules’ usage for exosome detection. In this study, for the first time, we have proposed a novel strategy of using Affibody functionalized microbeads (AffiBeads) for exosome detection with a high degree of efficiency. As a proof-of-concept, anti-EGFR-AffiBeads were fabricated and applied to capture and detect human lung A549 cancer cell-derived EGFR-positive exosomes using flow cytometry and fluorescent microscopy. Moreover, the capture efficiency of the AffiBeads were compared with its counterpart antibody. Our results showed that the Affibody probe had a detection limit of 15.6 ng exosomes per mL (~12 exosomes per AffiBead). The approach proposed in the current study can be used for sensitive detection of low expression level markers on tumor-derived exosomes, providing a basis for early-stage cancer diagnosis.Nima SayyadiSareh ZhandSajad Razavi BazazMajid Ebrahimi WarkianiMDPI AGarticleAffibodycancer-derived exosomeexosome biomarkerBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12014, p 12014 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Affibody cancer-derived exosome exosome biomarker Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	Affibody cancer-derived exosome exosome biomarker Biology (General) QH301-705.5 Chemistry QD1-999 Nima Sayyadi Sareh Zhand Sajad Razavi Bazaz Majid Ebrahimi Warkiani Affibody Functionalized Beads for the Highly Sensitive Detection of Cancer Cell-Derived Exosomes
description	Exosomes belong to the class of extracellular vesicles of endocytic origin, which are regarded as a promising source of cancer biomarkers in liquid biopsy. As a result, an accurate, sensitive, and specific quantification of these nano-sized particles is of significant importance. Affinity-based approaches are recognized as the most valuable technique for exosome isolation and characterization. Indeed, Affibody biomolecules are a type of protein scaffold engineered with small size and enjoy the features of high thermal stability, affinity, and specificity. While the utilization of antibodies, aptamers, and other biologically active substances for exosome detection has been reported widely, there are no reports describing Affibody molecules’ usage for exosome detection. In this study, for the first time, we have proposed a novel strategy of using Affibody functionalized microbeads (AffiBeads) for exosome detection with a high degree of efficiency. As a proof-of-concept, anti-EGFR-AffiBeads were fabricated and applied to capture and detect human lung A549 cancer cell-derived EGFR-positive exosomes using flow cytometry and fluorescent microscopy. Moreover, the capture efficiency of the AffiBeads were compared with its counterpart antibody. Our results showed that the Affibody probe had a detection limit of 15.6 ng exosomes per mL (~12 exosomes per AffiBead). The approach proposed in the current study can be used for sensitive detection of low expression level markers on tumor-derived exosomes, providing a basis for early-stage cancer diagnosis.
format	article
author	Nima Sayyadi Sareh Zhand Sajad Razavi Bazaz Majid Ebrahimi Warkiani
author_facet	Nima Sayyadi Sareh Zhand Sajad Razavi Bazaz Majid Ebrahimi Warkiani
author_sort	Nima Sayyadi
title	Affibody Functionalized Beads for the Highly Sensitive Detection of Cancer Cell-Derived Exosomes
title_short	Affibody Functionalized Beads for the Highly Sensitive Detection of Cancer Cell-Derived Exosomes
title_full	Affibody Functionalized Beads for the Highly Sensitive Detection of Cancer Cell-Derived Exosomes
title_fullStr	Affibody Functionalized Beads for the Highly Sensitive Detection of Cancer Cell-Derived Exosomes
title_full_unstemmed	Affibody Functionalized Beads for the Highly Sensitive Detection of Cancer Cell-Derived Exosomes
title_sort	affibody functionalized beads for the highly sensitive detection of cancer cell-derived exosomes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ff806c05a38440a49a04cbcc16fb1329
work_keys_str_mv	AT nimasayyadi affibodyfunctionalizedbeadsforthehighlysensitivedetectionofcancercellderivedexosomes AT sarehzhand affibodyfunctionalizedbeadsforthehighlysensitivedetectionofcancercellderivedexosomes AT sajadrazavibazaz affibodyfunctionalizedbeadsforthehighlysensitivedetectionofcancercellderivedexosomes AT majidebrahimiwarkiani affibodyfunctionalizedbeadsforthehighlysensitivedetectionofcancercellderivedexosomes
_version_	1718432157633871872

Affibody Functionalized Beads for the Highly Sensitive Detection of Cancer Cell-Derived Exosomes

Ejemplares similares