Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide

Katsutoshi Kokubun,1,2 Sachiko Matsumura,1 Masako Yudasaka,3,4 Sumio Iijima,3,4 Kiyotaka Shiba1 1Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan; 2Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan; 3Nanomaterials Rese...

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Autores principales:	Kokubun K, Matsumura S, Yudasaka M, Iijima S, Shiba K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	drug carrier drug delivery carbon nanomaterial carbon nanohorn peptide aptamer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ff858d7220114030801e151a0a52420e
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spelling	oai:doaj.org-article:ff858d7220114030801e151a0a52420e2021-12-02T00:48:21ZImmobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide1178-2013https://doaj.org/article/ff858d7220114030801e151a0a52420e2018-03-01T00:00:00Zhttps://www.dovepress.com/immobilization-of-a-carbon-nanomaterial-based-localized-drug-release-s-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Katsutoshi Kokubun,1,2 Sachiko Matsumura,1 Masako Yudasaka,3,4 Sumio Iijima,3,4 Kiyotaka Shiba1 1Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan; 2Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan; 3Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan; 4Graduate School of Science and Technology, Meijo University, Nagoya, Japan Introduction: Inorganic materials are widely used in medical devices, such as artificial hearts, vessels, and joints, in stents, and as nanocarriers for drug-delivery systems. Carbon nanomaterials are of particular interest due to their biological inertness and their capability to accommodate molecules. Several attempts have been proposed, in which carbon nanomaterials are used as nanocarriers for the systemic delivery of drugs.Materials and methods: We developed a drug-delivery system in which oxidized single-walled carbon nanohorns (oxSWNHs) were immobilized on a titanium (Ti) surface using material-binding peptides to enable localized drug delivery. For this purpose, we utilized a bispecific peptidic aptamer comprising a core sequence of a Ti-binding peptide and a SWNH-binding peptide to immobilize oxSWNHs on Ti.Results: Scanning electron microscopy was used to confirm the presence of oxSWNHs adsorbed onto the Ti surface, and a quartz crystal microbalance was used to evaluate the binding process during oxSWNH adsorption. The oxSWNHs-ornamented Ti substrate was nontoxic to cells and released biologically active dexamethasone over a sustained period.Conclusion: This oxSWNHs-immobilized system can be used to modify the surface of Ti in implants and be loaded with drugs that stimulate osteogenesis and bone regeneration. Keywords: drug carrier, drug delivery, carbon nanomaterial, carbon nanohorn, peptide aptamerKokubun KMatsumura SYudasaka MIijima SShiba KDove Medical Pressarticledrug carrierdrug deliverycarbon nanomaterialcarbon nanohornpeptide aptamerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 1643-1652 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	drug carrier drug delivery carbon nanomaterial carbon nanohorn peptide aptamer Medicine (General) R5-920
spellingShingle	drug carrier drug delivery carbon nanomaterial carbon nanohorn peptide aptamer Medicine (General) R5-920 Kokubun K Matsumura S Yudasaka M Iijima S Shiba K Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide
description	Katsutoshi Kokubun,1,2 Sachiko Matsumura,1 Masako Yudasaka,3,4 Sumio Iijima,3,4 Kiyotaka Shiba1 1Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan; 2Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan; 3Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan; 4Graduate School of Science and Technology, Meijo University, Nagoya, Japan Introduction: Inorganic materials are widely used in medical devices, such as artificial hearts, vessels, and joints, in stents, and as nanocarriers for drug-delivery systems. Carbon nanomaterials are of particular interest due to their biological inertness and their capability to accommodate molecules. Several attempts have been proposed, in which carbon nanomaterials are used as nanocarriers for the systemic delivery of drugs.Materials and methods: We developed a drug-delivery system in which oxidized single-walled carbon nanohorns (oxSWNHs) were immobilized on a titanium (Ti) surface using material-binding peptides to enable localized drug delivery. For this purpose, we utilized a bispecific peptidic aptamer comprising a core sequence of a Ti-binding peptide and a SWNH-binding peptide to immobilize oxSWNHs on Ti.Results: Scanning electron microscopy was used to confirm the presence of oxSWNHs adsorbed onto the Ti surface, and a quartz crystal microbalance was used to evaluate the binding process during oxSWNH adsorption. The oxSWNHs-ornamented Ti substrate was nontoxic to cells and released biologically active dexamethasone over a sustained period.Conclusion: This oxSWNHs-immobilized system can be used to modify the surface of Ti in implants and be loaded with drugs that stimulate osteogenesis and bone regeneration. Keywords: drug carrier, drug delivery, carbon nanomaterial, carbon nanohorn, peptide aptamer
format	article
author	Kokubun K Matsumura S Yudasaka M Iijima S Shiba K
author_facet	Kokubun K Matsumura S Yudasaka M Iijima S Shiba K
author_sort	Kokubun K
title	Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide
title_short	Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide
title_full	Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide
title_fullStr	Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide
title_full_unstemmed	Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide
title_sort	immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/ff858d7220114030801e151a0a52420e
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Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide

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