CHEMICAL COMPOSITION AND ANTI-IRRITANT CAPACITY OF WHOLE BODY EXTRACTS OF Ulomoides dermestoides (COLEOPTERA, TENEBRIONIDAE)

Background: In traditional medicine of Central and South America, the tenebrionid beetle Ulomoides dermestoides is used as an a phrodisiac, for the treatment of inflammatory diseases and cancer. Recently was reported cytotoxic and genotoxic properties of non-polar extract of U. dermestoides; also an...

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Autores principales: Dary L. MENDOZA M., Stephanie SAAVEDRA A.
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Publicado: Universidad de Antioquia 2013
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spelling oai:doaj.org-article:ff9328e1ff884431aa01289c7129a2152021-12-02T04:49:20ZCHEMICAL COMPOSITION AND ANTI-IRRITANT CAPACITY OF WHOLE BODY EXTRACTS OF Ulomoides dermestoides (COLEOPTERA, TENEBRIONIDAE)0121-4004https://doaj.org/article/ff9328e1ff884431aa01289c7129a2152013-04-01T00:00:00Zhttp://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-40042013000100005&lng=en&tlng=enhttps://doaj.org/toc/0121-4004Background: In traditional medicine of Central and South America, the tenebrionid beetle Ulomoides dermestoides is used as an a phrodisiac, for the treatment of inflammatory diseases and cancer. Recently was reported cytotoxic and genotoxic properties of non-polar extract of U. dermestoides; also anti-inflammatory and immunomodulatory activity of aqueous whole body extract of beetle was reported, it suggests the existence of components with potential pharmacology use. On the other hand, it is necessary to identify those polar and non-polar extracts of U. dermestoides with anti-irritant properties for the membranes and blood vessels, which will be used in subsequence biological test and clinical assays. Objectives: The purpose of this research was to identify the chemical composition of methanolic and hexanic extracts of U. dermestoides, and to assess their anti-irritant capacity. Methods: The extracts were obtained from adult beetles of U. dermestoides. The chemical composition of the extracts was determined by gas chromatography-mass spectrometry (GC-MS) and the anti-irritant effect of each extract was evaluated by means of a modified assay of irritation of the chorioallantoic membrane (CAM) of fertilized chicken eggs (HET-CAM); the results were expressed as irritation index (IR). Results: Six common compounds were identified in both extracts: limonene, myristic, palmitic, estearic, oleic, and linoleic fatty acids. But in the alone methanolic extract were found: 1-pentadecanol, alpha-pinene, beta-phellandrene and alpha-terpinene, whereas in the hexanic extract were found: 2-methyl-p-benzoquinone, 2,4-dihidroxy-1-ethylbenzene, 2,5-dimethylquinone, saturated and unsaturated hydrocarbons and alcohols. The methanolic extract of U. dermestoides showed potential anti-irritant effect in the HET-CAM test (IR = 3.09 ± 0.11), similar to that observed with Nimesulida (IR = 2.05 ± 0.14), a non-steroid anti-inflammatory drug (NSAID) used as positive control for irritation inhibition reaction. The hexanic extract did not show anti-irritant capacity. Conclusions: The results demonstrated the anti-irritant effect of the methanolic extracts of U. dermestoides that could be attributed to compounds with anti-inflammatory activity as oleic acid and limonene.Dary L. MENDOZA M.Stephanie SAAVEDRA A.Universidad de AntioquiaarticleUlomoides dermestoidesescarabajosmetabolitosbioensayomembrana corioalantóideFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 20, Iss 1, Pp 41-48 (2013)
institution DOAJ
collection DOAJ
language EN
topic Ulomoides dermestoides
escarabajos
metabolitos
bioensayo
membrana corioalantóide
Food processing and manufacture
TP368-456
Pharmaceutical industry
HD9665-9675
spellingShingle Ulomoides dermestoides
escarabajos
metabolitos
bioensayo
membrana corioalantóide
Food processing and manufacture
TP368-456
Pharmaceutical industry
HD9665-9675
Dary L. MENDOZA M.
Stephanie SAAVEDRA A.
CHEMICAL COMPOSITION AND ANTI-IRRITANT CAPACITY OF WHOLE BODY EXTRACTS OF Ulomoides dermestoides (COLEOPTERA, TENEBRIONIDAE)
description Background: In traditional medicine of Central and South America, the tenebrionid beetle Ulomoides dermestoides is used as an a phrodisiac, for the treatment of inflammatory diseases and cancer. Recently was reported cytotoxic and genotoxic properties of non-polar extract of U. dermestoides; also anti-inflammatory and immunomodulatory activity of aqueous whole body extract of beetle was reported, it suggests the existence of components with potential pharmacology use. On the other hand, it is necessary to identify those polar and non-polar extracts of U. dermestoides with anti-irritant properties for the membranes and blood vessels, which will be used in subsequence biological test and clinical assays. Objectives: The purpose of this research was to identify the chemical composition of methanolic and hexanic extracts of U. dermestoides, and to assess their anti-irritant capacity. Methods: The extracts were obtained from adult beetles of U. dermestoides. The chemical composition of the extracts was determined by gas chromatography-mass spectrometry (GC-MS) and the anti-irritant effect of each extract was evaluated by means of a modified assay of irritation of the chorioallantoic membrane (CAM) of fertilized chicken eggs (HET-CAM); the results were expressed as irritation index (IR). Results: Six common compounds were identified in both extracts: limonene, myristic, palmitic, estearic, oleic, and linoleic fatty acids. But in the alone methanolic extract were found: 1-pentadecanol, alpha-pinene, beta-phellandrene and alpha-terpinene, whereas in the hexanic extract were found: 2-methyl-p-benzoquinone, 2,4-dihidroxy-1-ethylbenzene, 2,5-dimethylquinone, saturated and unsaturated hydrocarbons and alcohols. The methanolic extract of U. dermestoides showed potential anti-irritant effect in the HET-CAM test (IR = 3.09 ± 0.11), similar to that observed with Nimesulida (IR = 2.05 ± 0.14), a non-steroid anti-inflammatory drug (NSAID) used as positive control for irritation inhibition reaction. The hexanic extract did not show anti-irritant capacity. Conclusions: The results demonstrated the anti-irritant effect of the methanolic extracts of U. dermestoides that could be attributed to compounds with anti-inflammatory activity as oleic acid and limonene.
format article
author Dary L. MENDOZA M.
Stephanie SAAVEDRA A.
author_facet Dary L. MENDOZA M.
Stephanie SAAVEDRA A.
author_sort Dary L. MENDOZA M.
title CHEMICAL COMPOSITION AND ANTI-IRRITANT CAPACITY OF WHOLE BODY EXTRACTS OF Ulomoides dermestoides (COLEOPTERA, TENEBRIONIDAE)
title_short CHEMICAL COMPOSITION AND ANTI-IRRITANT CAPACITY OF WHOLE BODY EXTRACTS OF Ulomoides dermestoides (COLEOPTERA, TENEBRIONIDAE)
title_full CHEMICAL COMPOSITION AND ANTI-IRRITANT CAPACITY OF WHOLE BODY EXTRACTS OF Ulomoides dermestoides (COLEOPTERA, TENEBRIONIDAE)
title_fullStr CHEMICAL COMPOSITION AND ANTI-IRRITANT CAPACITY OF WHOLE BODY EXTRACTS OF Ulomoides dermestoides (COLEOPTERA, TENEBRIONIDAE)
title_full_unstemmed CHEMICAL COMPOSITION AND ANTI-IRRITANT CAPACITY OF WHOLE BODY EXTRACTS OF Ulomoides dermestoides (COLEOPTERA, TENEBRIONIDAE)
title_sort chemical composition and anti-irritant capacity of whole body extracts of ulomoides dermestoides (coleoptera, tenebrionidae)
publisher Universidad de Antioquia
publishDate 2013
url https://doaj.org/article/ff9328e1ff884431aa01289c7129a215
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AT stephaniesaavedraa chemicalcompositionandantiirritantcapacityofwholebodyextractsofulomoidesdermestoidescoleopteratenebrionidae
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