Simultaneous and stoichiometric purification of hundreds of oligonucleotides

Chemically synthesized DNA oligonucleotides (oligos) require purification to remove truncated species. Here, the authors developed a high-throughput method for oligo purification that also normalises the concentrations of the oligos in the final samples.

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Detalles Bibliográficos
Autores principales: Alessandro Pinto, Sherry X. Chen, David Yu Zhang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
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Acceso en línea:https://doaj.org/article/ff9369e024ac4f13b1f3c810f3d60847
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Descripción
Sumario:Chemically synthesized DNA oligonucleotides (oligos) require purification to remove truncated species. Here, the authors developed a high-throughput method for oligo purification that also normalises the concentrations of the oligos in the final samples.