Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.

The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T) and one...

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Autores principales:	Elaine M S Dorneles, Jordana A Santana, Dayana Ribeiro, Fernanda Alves Dorella, Alessandro S Guimarães, Mohamed S Moawad, Salah A Selim, Ana Luiza M Garaldi, Anderson Miyoshi, Márcio G Ribeiro, Aurora M G Gouveia, Vasco Azevedo, Marcos B Heinemann, Andrey P Lage
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff95efcdbe8b476089f32c131cc1bbd3
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spelling	oai:doaj.org-article:ff95efcdbe8b476089f32c131cc1bbd32021-11-18T08:17:01ZEvaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.1932-620310.1371/journal.pone.0098758https://doaj.org/article/ff95efcdbe8b476089f32c131cc1bbd32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24901343/?tool=EBIhttps://doaj.org/toc/1932-6203The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.Elaine M S DornelesJordana A SantanaDayana RibeiroFernanda Alves DorellaAlessandro S GuimarãesMohamed S MoawadSalah A SelimAna Luiza M GaraldiAnderson MiyoshiMárcio G RibeiroAurora M G GouveiaVasco AzevedoMarcos B HeinemannAndrey P LagePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98758 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elaine M S Dorneles Jordana A Santana Dayana Ribeiro Fernanda Alves Dorella Alessandro S Guimarães Mohamed S Moawad Salah A Selim Ana Luiza M Garaldi Anderson Miyoshi Márcio G Ribeiro Aurora M G Gouveia Vasco Azevedo Marcos B Heinemann Andrey P Lage Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.
description	The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
format	article
author	Elaine M S Dorneles Jordana A Santana Dayana Ribeiro Fernanda Alves Dorella Alessandro S Guimarães Mohamed S Moawad Salah A Selim Ana Luiza M Garaldi Anderson Miyoshi Márcio G Ribeiro Aurora M G Gouveia Vasco Azevedo Marcos B Heinemann Andrey P Lage
author_facet	Elaine M S Dorneles Jordana A Santana Dayana Ribeiro Fernanda Alves Dorella Alessandro S Guimarães Mohamed S Moawad Salah A Selim Ana Luiza M Garaldi Anderson Miyoshi Márcio G Ribeiro Aurora M G Gouveia Vasco Azevedo Marcos B Heinemann Andrey P Lage
author_sort	Elaine M S Dorneles
title	Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.
title_short	Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.
title_full	Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.
title_fullStr	Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.
title_full_unstemmed	Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.
title_sort	evaluation of eric-pcr as genotyping method for corynebacterium pseudotuberculosis isolates.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ff95efcdbe8b476089f32c131cc1bbd3
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Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.

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