Massive Gene Flux Drives Genome Diversity between Sympatric <italic toggle="yes">Streptomyces</italic> Conspecifics

ABSTRACT In this work, by comparing genomes of closely related individuals of Streptomyces isolated at a spatial microscale (millimeters or centimeters), we investigated the extent and impact of horizontal gene transfer in the diversification of a natural Streptomyces population. We show that despit...

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Autores principales:	Abdoul-Razak Tidjani, Jean-Noël Lorenzi, Maxime Toussaint, Erwin van Dijk, Delphine Naquin, Olivier Lespinet, Cyril Bontemps, Pierre Leblond
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Streptomyces conjugation gene transfer plasticity population Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ff96284dea1648d4a50d21b447b6311a
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spelling	oai:doaj.org-article:ff96284dea1648d4a50d21b447b6311a2021-11-15T15:59:41ZMassive Gene Flux Drives Genome Diversity between Sympatric <italic toggle="yes">Streptomyces</italic> Conspecifics10.1128/mBio.01533-192150-7511https://doaj.org/article/ff96284dea1648d4a50d21b447b6311a2019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01533-19https://doaj.org/toc/2150-7511ABSTRACT In this work, by comparing genomes of closely related individuals of Streptomyces isolated at a spatial microscale (millimeters or centimeters), we investigated the extent and impact of horizontal gene transfer in the diversification of a natural Streptomyces population. We show that despite these conspecific strains sharing a recent common ancestor, all harbored significantly different gene contents, implying massive and rapid gene flux. The accessory genome of the strains was distributed across insertion/deletion events (indels) ranging from one to several hundreds of genes. Indels were preferentially located in the arms of the linear chromosomes (ca. 12 Mb) and appeared to form recombination hot spots. Some of them harbored biosynthetic gene clusters (BGCs) whose products confer an inhibitory capacity and may constitute public goods that can favor the cohesiveness of the bacterial population. Moreover, a significant proportion of these variable genes were either plasmid borne or harbored signatures of actinomycete integrative and conjugative elements (AICEs). We propose that conjugation is the main driver for the indel flux and diversity in Streptomyces populations. IMPORTANCE Horizontal gene transfer is a rapid and efficient way to diversify bacterial gene pools. Currently, little is known about this gene flux within natural soil populations. Using comparative genomics of Streptomyces strains belonging to the same species and isolated at microscale, we reveal frequent transfer of a significant fraction of the pangenome. We show that it occurs at a time scale enabling the population to diversify and to cope with its changing environment, notably, through the production of public goods.Abdoul-Razak TidjaniJean-Noël LorenziMaxime ToussaintErwin van DijkDelphine NaquinOlivier LespinetCyril BontempsPierre LeblondAmerican Society for MicrobiologyarticleStreptomycesconjugationgene transferplasticitypopulationMicrobiologyQR1-502ENmBio, Vol 10, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Streptomyces conjugation gene transfer plasticity population Microbiology QR1-502
spellingShingle	Streptomyces conjugation gene transfer plasticity population Microbiology QR1-502 Abdoul-Razak Tidjani Jean-Noël Lorenzi Maxime Toussaint Erwin van Dijk Delphine Naquin Olivier Lespinet Cyril Bontemps Pierre Leblond Massive Gene Flux Drives Genome Diversity between Sympatric <italic toggle="yes">Streptomyces</italic> Conspecifics
description	ABSTRACT In this work, by comparing genomes of closely related individuals of Streptomyces isolated at a spatial microscale (millimeters or centimeters), we investigated the extent and impact of horizontal gene transfer in the diversification of a natural Streptomyces population. We show that despite these conspecific strains sharing a recent common ancestor, all harbored significantly different gene contents, implying massive and rapid gene flux. The accessory genome of the strains was distributed across insertion/deletion events (indels) ranging from one to several hundreds of genes. Indels were preferentially located in the arms of the linear chromosomes (ca. 12 Mb) and appeared to form recombination hot spots. Some of them harbored biosynthetic gene clusters (BGCs) whose products confer an inhibitory capacity and may constitute public goods that can favor the cohesiveness of the bacterial population. Moreover, a significant proportion of these variable genes were either plasmid borne or harbored signatures of actinomycete integrative and conjugative elements (AICEs). We propose that conjugation is the main driver for the indel flux and diversity in Streptomyces populations. IMPORTANCE Horizontal gene transfer is a rapid and efficient way to diversify bacterial gene pools. Currently, little is known about this gene flux within natural soil populations. Using comparative genomics of Streptomyces strains belonging to the same species and isolated at microscale, we reveal frequent transfer of a significant fraction of the pangenome. We show that it occurs at a time scale enabling the population to diversify and to cope with its changing environment, notably, through the production of public goods.
format	article
author	Abdoul-Razak Tidjani Jean-Noël Lorenzi Maxime Toussaint Erwin van Dijk Delphine Naquin Olivier Lespinet Cyril Bontemps Pierre Leblond
author_facet	Abdoul-Razak Tidjani Jean-Noël Lorenzi Maxime Toussaint Erwin van Dijk Delphine Naquin Olivier Lespinet Cyril Bontemps Pierre Leblond
author_sort	Abdoul-Razak Tidjani
title	Massive Gene Flux Drives Genome Diversity between Sympatric <italic toggle="yes">Streptomyces</italic> Conspecifics
title_short	Massive Gene Flux Drives Genome Diversity between Sympatric <italic toggle="yes">Streptomyces</italic> Conspecifics
title_full	Massive Gene Flux Drives Genome Diversity between Sympatric <italic toggle="yes">Streptomyces</italic> Conspecifics
title_fullStr	Massive Gene Flux Drives Genome Diversity between Sympatric <italic toggle="yes">Streptomyces</italic> Conspecifics
title_full_unstemmed	Massive Gene Flux Drives Genome Diversity between Sympatric <italic toggle="yes">Streptomyces</italic> Conspecifics
title_sort	massive gene flux drives genome diversity between sympatric <italic toggle="yes">streptomyces</italic> conspecifics
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/ff96284dea1648d4a50d21b447b6311a
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Massive Gene Flux Drives Genome Diversity between Sympatric <italic toggle="yes">Streptomyces</italic> Conspecifics

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