Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells

Many aptamer-fluorophore complexes suffer from low quantum yield and low extinction coefficients limiting their usability. Here the authors isolate new Mango aptamers with improved fluorescent properties for both fixed and live-cell imaging by using competitive ligand binding microfluidic selection.

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Autores principales:	Alexis Autour, Sunny C. Y. Jeng, Adam D. Cawte, Amir Abdolahzadeh, Angela Galli, Shanker S. S. Panchapakesan, David Rueda, Michael Ryckelynck, Peter J. Unrau
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ffa23cd158524e5b94c7284e2e879efb
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spelling	oai:doaj.org-article:ffa23cd158524e5b94c7284e2e879efb2021-12-02T15:34:03ZFluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells10.1038/s41467-018-02993-82041-1723https://doaj.org/article/ffa23cd158524e5b94c7284e2e879efb2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-02993-8https://doaj.org/toc/2041-1723Many aptamer-fluorophore complexes suffer from low quantum yield and low extinction coefficients limiting their usability. Here the authors isolate new Mango aptamers with improved fluorescent properties for both fixed and live-cell imaging by using competitive ligand binding microfluidic selection.Alexis AutourSunny C. Y. JengAdam D. CawteAmir AbdolahzadehAngela GalliShanker S. S. PanchapakesanDavid RuedaMichael RyckelynckPeter J. UnrauNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alexis Autour Sunny C. Y. Jeng Adam D. Cawte Amir Abdolahzadeh Angela Galli Shanker S. S. Panchapakesan David Rueda Michael Ryckelynck Peter J. Unrau Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells
description	Many aptamer-fluorophore complexes suffer from low quantum yield and low extinction coefficients limiting their usability. Here the authors isolate new Mango aptamers with improved fluorescent properties for both fixed and live-cell imaging by using competitive ligand binding microfluidic selection.
format	article
author	Alexis Autour Sunny C. Y. Jeng Adam D. Cawte Amir Abdolahzadeh Angela Galli Shanker S. S. Panchapakesan David Rueda Michael Ryckelynck Peter J. Unrau
author_facet	Alexis Autour Sunny C. Y. Jeng Adam D. Cawte Amir Abdolahzadeh Angela Galli Shanker S. S. Panchapakesan David Rueda Michael Ryckelynck Peter J. Unrau
author_sort	Alexis Autour
title	Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells
title_short	Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells
title_full	Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells
title_fullStr	Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells
title_full_unstemmed	Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells
title_sort	fluorogenic rna mango aptamers for imaging small non-coding rnas in mammalian cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ffa23cd158524e5b94c7284e2e879efb
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Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells

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