Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria

Abstract Reduced graphene oxide (rGO) is a promising antibacterial material, the efficacy of which can be further enhanced by the addition of silver nanoparticles (nAg). In this study, the mechanisms of antibacterial activity of rGO–nAg nanocomposite against several important human pathogenic multi-...

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Autores principales:	Karthika Prasad, G. S. Lekshmi, Kola Ostrikov, Vanessa Lussini, James Blinco, Mandhakini Mohandas, Krasimir Vasilev, Steven Bottle, Kateryna Bazaka, Kostya Ostrikov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ffa46bce39ec4e518486efff8f1141a8
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spelling	oai:doaj.org-article:ffa46bce39ec4e518486efff8f1141a82021-12-02T15:05:23ZSynergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria10.1038/s41598-017-01669-52045-2322https://doaj.org/article/ffa46bce39ec4e518486efff8f1141a82017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01669-5https://doaj.org/toc/2045-2322Abstract Reduced graphene oxide (rGO) is a promising antibacterial material, the efficacy of which can be further enhanced by the addition of silver nanoparticles (nAg). In this study, the mechanisms of antibacterial activity of rGO–nAg nanocomposite against several important human pathogenic multi-drug resistant bacteria, namely Gram-positive coccal Staphylococcus aureus and Gram-negative rod-shaped Escherichia coli and Proteus mirabilis are investigated. At the same concentration (100 µg/ml), rGO–nAg nanocomposite was significantly more effective against all three pathogens than either rGO or nAg. The nanocomposite was equally active against P. mirabilis and S. aureus as systemic antibiotic nitrofurantoin, and significantly more effective against E. coli. Importantly, the inhibition was much faster in the case of rGO–nAg nanocomposite compared to nitrofurantoin, attributed to the synergistic effects of rGO–nAg mediated contact killing and oxidative stress. This study may provide new insights for the better understanding of antibacterial actions of rGO–nAg nanocomposite and for the better designing of graphene-based antibiotics or other biomedical applications.Karthika PrasadG. S. LekshmiKola OstrikovVanessa LussiniJames BlincoMandhakini MohandasKrasimir VasilevSteven BottleKateryna BazakaKostya OstrikovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karthika Prasad G. S. Lekshmi Kola Ostrikov Vanessa Lussini James Blinco Mandhakini Mohandas Krasimir Vasilev Steven Bottle Kateryna Bazaka Kostya Ostrikov Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria
description	Abstract Reduced graphene oxide (rGO) is a promising antibacterial material, the efficacy of which can be further enhanced by the addition of silver nanoparticles (nAg). In this study, the mechanisms of antibacterial activity of rGO–nAg nanocomposite against several important human pathogenic multi-drug resistant bacteria, namely Gram-positive coccal Staphylococcus aureus and Gram-negative rod-shaped Escherichia coli and Proteus mirabilis are investigated. At the same concentration (100 µg/ml), rGO–nAg nanocomposite was significantly more effective against all three pathogens than either rGO or nAg. The nanocomposite was equally active against P. mirabilis and S. aureus as systemic antibiotic nitrofurantoin, and significantly more effective against E. coli. Importantly, the inhibition was much faster in the case of rGO–nAg nanocomposite compared to nitrofurantoin, attributed to the synergistic effects of rGO–nAg mediated contact killing and oxidative stress. This study may provide new insights for the better understanding of antibacterial actions of rGO–nAg nanocomposite and for the better designing of graphene-based antibiotics or other biomedical applications.
format	article
author	Karthika Prasad G. S. Lekshmi Kola Ostrikov Vanessa Lussini James Blinco Mandhakini Mohandas Krasimir Vasilev Steven Bottle Kateryna Bazaka Kostya Ostrikov
author_facet	Karthika Prasad G. S. Lekshmi Kola Ostrikov Vanessa Lussini James Blinco Mandhakini Mohandas Krasimir Vasilev Steven Bottle Kateryna Bazaka Kostya Ostrikov
author_sort	Karthika Prasad
title	Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria
title_short	Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria
title_full	Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria
title_fullStr	Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria
title_full_unstemmed	Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria
title_sort	synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against gram-positive and gram-negative bacteria
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ffa46bce39ec4e518486efff8f1141a8
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Synergic bactericidal effects of reduced graphene oxide and silver nanoparticles against Gram-positive and Gram-negative bacteria

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