A Simple and Universal System for Gene Manipulation in <named-content content-type="genus-species">Aspergillus fumigatus</named-content>: <italic toggle="yes">In Vitro</italic>-Assembled Cas9-Guide RNA Ribonucleoproteins Coupled with Microhomology Repair Templates

A Simple and Universal System for Gene Manipulation in <named-content content-type="genus-species">Aspergillus fumigatus</named-content>: <italic toggle="yes">In Vitro</italic>-Assembled Cas9-Guide RNA Ribonucleoproteins Coupled with Microhomology Repair Templates

ABSTRACT CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 is a novel genome-editing system that has been successfully established in Aspergillus fumigatus. However, the current state of the technology relies heavily on DNA-based expression cassettes for delivering Cas9 and the...

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Bibliographic Details
Main Authors: Qusai Al Abdallah, Wenbo Ge, Jarrod R. Fortwendel
Format: article
Language:EN
Published: American Society for Microbiology 2017
Subjects:
Aspergillus fumigatus
CRISPR-Cas9
gene deletion
genome editing
in vitro assembly
pksP gene
Microbiology
QR1-502
Online Access:https://doaj.org/article/ffb15b96551c4915a89328e7d09458a1
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