A Simple and Universal System for Gene Manipulation in <named-content content-type="genus-species">Aspergillus fumigatus</named-content>: <italic toggle="yes">In Vitro</italic>-Assembled Cas9-Guide RNA Ribonucleoproteins Coupled with Microhomology Repair Templates
ABSTRACT CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 is a novel genome-editing system that has been successfully established in Aspergillus fumigatus. However, the current state of the technology relies heavily on DNA-based expression cassettes for delivering Cas9 and the...
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| Main Authors: | , , |
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| Format: | article |
| Language: | EN |
| Published: |
American Society for Microbiology
2017
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| Subjects: | |
| Online Access: | https://doaj.org/article/ffb15b96551c4915a89328e7d09458a1 |
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