Galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane

Abstract Galectin-3 (Gal-3) controls intercellular and cell-extracellular matrix interactions during immunological responses. In chronic inflammation, Gal-3 is associated with fibrotic events, regulates B cell differentiation and delays lupus progression. Gal-3 deficient mice (Lgals3−/−) have intens...

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Autores principales:	F. S. Lemos, J. X. Pereira, V. F. Carvalho, E. S. Bernardes, R. Chammas, T. M. Pereira, R. S. Carvalho, R. Luisetto, M. C. El-Cheikh, S. Calil-Elias, F. L. Oliveira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ffbe4549b48d402f84e8c76c7a6c9bd0
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spelling	oai:doaj.org-article:ffbe4549b48d402f84e8c76c7a6c9bd02021-12-02T15:09:45ZGalectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane10.1038/s41598-019-50564-82045-2322https://doaj.org/article/ffbe4549b48d402f84e8c76c7a6c9bd02019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-50564-8https://doaj.org/toc/2045-2322Abstract Galectin-3 (Gal-3) controls intercellular and cell-extracellular matrix interactions during immunological responses. In chronic inflammation, Gal-3 is associated with fibrotic events, regulates B cell differentiation and delays lupus progression. Gal-3 deficient mice (Lgals3−/−) have intense germinal center formation and atypical plasma cell generation correlated to high levels IgG, IgE, and IgA. Here, we used pristane (2,6,10,14-tetramethylpentadecane) to induce lupus-like syndrome in Lgals3−/− and Lgals3+/+ BALB/c mice. Mesentery and peritoneal cells were monitored because promptly react to pristane injected in the peritoneal cavity. For the first time, mesenteric tissues have been associated to the pathogenesis of experimental lupus-like syndrome. In Lgals3+/+ pristane-induced mice, mesentery was hallmarked by intense fibrogranulomatous reaction restricted to submesothelial regions and organized niches containing macrophages and B lymphocytes and plasma cells. In contrast, Lgals3−/− pristane-treated mice had diffuse mesenteric fibrosis affecting submesothelium and peripheral tissues, atypical M1/M2 macrophage polarization and significant DLL1+ cells expansion, suggesting possible involvement of Notch/Delta pathways in the disease. Early inflammatory reaction to pristane was characterized by significant disturbances on monocyte recruitment, macrophage differentiation and dendritic cell (DC) responses in the peritoneal cavity of pristane-induced Lgals3−/− mice. A correlative analysis showed that mesenteric damages in the absence of Gal-3 were directly associated with severe portal inflammation and hepatitis. In conclusion, it has suggested that Gal-3 orchestrates histological organization in the mesentery and prevents lupoid hepatitis in experimental lupus-like syndrome by controlling macrophage polarization, Notch signaling pathways and DC differentiation in mesenteric structures.F. S. LemosJ. X. PereiraV. F. CarvalhoE. S. BernardesR. ChammasT. M. PereiraR. S. CarvalhoR. LuisettoM. C. El-CheikhS. Calil-EliasF. L. OliveiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q F. S. Lemos J. X. Pereira V. F. Carvalho E. S. Bernardes R. Chammas T. M. Pereira R. S. Carvalho R. Luisetto M. C. El-Cheikh S. Calil-Elias F. L. Oliveira Galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane
description	Abstract Galectin-3 (Gal-3) controls intercellular and cell-extracellular matrix interactions during immunological responses. In chronic inflammation, Gal-3 is associated with fibrotic events, regulates B cell differentiation and delays lupus progression. Gal-3 deficient mice (Lgals3−/−) have intense germinal center formation and atypical plasma cell generation correlated to high levels IgG, IgE, and IgA. Here, we used pristane (2,6,10,14-tetramethylpentadecane) to induce lupus-like syndrome in Lgals3−/− and Lgals3+/+ BALB/c mice. Mesentery and peritoneal cells were monitored because promptly react to pristane injected in the peritoneal cavity. For the first time, mesenteric tissues have been associated to the pathogenesis of experimental lupus-like syndrome. In Lgals3+/+ pristane-induced mice, mesentery was hallmarked by intense fibrogranulomatous reaction restricted to submesothelial regions and organized niches containing macrophages and B lymphocytes and plasma cells. In contrast, Lgals3−/− pristane-treated mice had diffuse mesenteric fibrosis affecting submesothelium and peripheral tissues, atypical M1/M2 macrophage polarization and significant DLL1+ cells expansion, suggesting possible involvement of Notch/Delta pathways in the disease. Early inflammatory reaction to pristane was characterized by significant disturbances on monocyte recruitment, macrophage differentiation and dendritic cell (DC) responses in the peritoneal cavity of pristane-induced Lgals3−/− mice. A correlative analysis showed that mesenteric damages in the absence of Gal-3 were directly associated with severe portal inflammation and hepatitis. In conclusion, it has suggested that Gal-3 orchestrates histological organization in the mesentery and prevents lupoid hepatitis in experimental lupus-like syndrome by controlling macrophage polarization, Notch signaling pathways and DC differentiation in mesenteric structures.
format	article
author	F. S. Lemos J. X. Pereira V. F. Carvalho E. S. Bernardes R. Chammas T. M. Pereira R. S. Carvalho R. Luisetto M. C. El-Cheikh S. Calil-Elias F. L. Oliveira
author_facet	F. S. Lemos J. X. Pereira V. F. Carvalho E. S. Bernardes R. Chammas T. M. Pereira R. S. Carvalho R. Luisetto M. C. El-Cheikh S. Calil-Elias F. L. Oliveira
author_sort	F. S. Lemos
title	Galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane
title_short	Galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane
title_full	Galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane
title_fullStr	Galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane
title_full_unstemmed	Galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane
title_sort	galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ffbe4549b48d402f84e8c76c7a6c9bd0
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Galectin-3 orchestrates the histology of mesentery and protects liver during lupus-like syndrome induced by pristane

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