A research study of the association between maternal microchimerism and systemic lupus erythematosus in adults: a comparison between patients and healthy controls based on single-nucleotide polymorphism using quantitative real-time PCR.

<h4>Background</h4>Naturally acquired microchimerism may arise in the mother and her child during pregnancy when bidirectional trafficking of cells occurs through the placental barrier. The occurrence of maternal microchimerism (maternal cells in the offspring) has been associated with s...

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Autores principales:	Anna Maria Jonsson Kanold, Elisabet Svenungsson, Iva Gunnarsson, Cecilia Götherström, Leonid Padyukov, Nikos Papadogiannakis, Mehmet Uzunel, Magnus Westgren
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ffc739b0757c4eacb5b9e78708bbf883
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spelling	oai:doaj.org-article:ffc739b0757c4eacb5b9e78708bbf8832021-11-18T08:55:42ZA research study of the association between maternal microchimerism and systemic lupus erythematosus in adults: a comparison between patients and healthy controls based on single-nucleotide polymorphism using quantitative real-time PCR.1932-620310.1371/journal.pone.0074534https://doaj.org/article/ffc739b0757c4eacb5b9e78708bbf8832013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24040274/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Naturally acquired microchimerism may arise in the mother and her child during pregnancy when bidirectional trafficking of cells occurs through the placental barrier. The occurrence of maternal microchimerism (maternal cells in the offspring) has been associated with several autoimmune diseases, especially in children. Systemic Lupus erythematosus (SLE) is an autoimmune disorder with a resemblance to graft-versus-host disease. The aim of this study was to investigate the association between maternal microchimerism in the blood and SLE.<h4>Methodology/principal findings</h4>Thirty-two patients with SLE, 17 healthy brothers of the patients, and an additional 12 unrelated healthy men were the subjects in this study. A single-nucleotide polymorphism unique to each mother was identified, and maternal microchimerism in the study group and in the control group was detected using a quantitative real-time polymerase chain reaction technique. No differences in the frequency or the concentration of maternal cells were apparent in the blood of patients with SLE or in that of the controls. Two patients and one control tested positive for maternal microchimerism, but the positive subjects were all negative at a follow-up 16 years later. The sensitivity of the method was estimated to 1/10.000.<h4>Conclusions/significance</h4>These results show no association between SLE and maternal microchimerism. The frequency of maternal microchimerism in the blood of adults overall may be lower than earlier reported.Anna Maria Jonsson KanoldElisabet SvenungssonIva GunnarssonCecilia GötherströmLeonid PadyukovNikos PapadogiannakisMehmet UzunelMagnus WestgrenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74534 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Maria Jonsson Kanold Elisabet Svenungsson Iva Gunnarsson Cecilia Götherström Leonid Padyukov Nikos Papadogiannakis Mehmet Uzunel Magnus Westgren A research study of the association between maternal microchimerism and systemic lupus erythematosus in adults: a comparison between patients and healthy controls based on single-nucleotide polymorphism using quantitative real-time PCR.
description	<h4>Background</h4>Naturally acquired microchimerism may arise in the mother and her child during pregnancy when bidirectional trafficking of cells occurs through the placental barrier. The occurrence of maternal microchimerism (maternal cells in the offspring) has been associated with several autoimmune diseases, especially in children. Systemic Lupus erythematosus (SLE) is an autoimmune disorder with a resemblance to graft-versus-host disease. The aim of this study was to investigate the association between maternal microchimerism in the blood and SLE.<h4>Methodology/principal findings</h4>Thirty-two patients with SLE, 17 healthy brothers of the patients, and an additional 12 unrelated healthy men were the subjects in this study. A single-nucleotide polymorphism unique to each mother was identified, and maternal microchimerism in the study group and in the control group was detected using a quantitative real-time polymerase chain reaction technique. No differences in the frequency or the concentration of maternal cells were apparent in the blood of patients with SLE or in that of the controls. Two patients and one control tested positive for maternal microchimerism, but the positive subjects were all negative at a follow-up 16 years later. The sensitivity of the method was estimated to 1/10.000.<h4>Conclusions/significance</h4>These results show no association between SLE and maternal microchimerism. The frequency of maternal microchimerism in the blood of adults overall may be lower than earlier reported.
format	article
author	Anna Maria Jonsson Kanold Elisabet Svenungsson Iva Gunnarsson Cecilia Götherström Leonid Padyukov Nikos Papadogiannakis Mehmet Uzunel Magnus Westgren
author_facet	Anna Maria Jonsson Kanold Elisabet Svenungsson Iva Gunnarsson Cecilia Götherström Leonid Padyukov Nikos Papadogiannakis Mehmet Uzunel Magnus Westgren
author_sort	Anna Maria Jonsson Kanold
title	A research study of the association between maternal microchimerism and systemic lupus erythematosus in adults: a comparison between patients and healthy controls based on single-nucleotide polymorphism using quantitative real-time PCR.
title_short	A research study of the association between maternal microchimerism and systemic lupus erythematosus in adults: a comparison between patients and healthy controls based on single-nucleotide polymorphism using quantitative real-time PCR.
title_full	A research study of the association between maternal microchimerism and systemic lupus erythematosus in adults: a comparison between patients and healthy controls based on single-nucleotide polymorphism using quantitative real-time PCR.
title_fullStr	A research study of the association between maternal microchimerism and systemic lupus erythematosus in adults: a comparison between patients and healthy controls based on single-nucleotide polymorphism using quantitative real-time PCR.
title_full_unstemmed	A research study of the association between maternal microchimerism and systemic lupus erythematosus in adults: a comparison between patients and healthy controls based on single-nucleotide polymorphism using quantitative real-time PCR.
title_sort	research study of the association between maternal microchimerism and systemic lupus erythematosus in adults: a comparison between patients and healthy controls based on single-nucleotide polymorphism using quantitative real-time pcr.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ffc739b0757c4eacb5b9e78708bbf883
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A research study of the association between maternal microchimerism and systemic lupus erythematosus in adults: a comparison between patients and healthy controls based on single-nucleotide polymorphism using quantitative real-time PCR.

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