NLRP3 inflammasome is a key player in human vulvovaginal disease caused by Candida albicans

Abstract The expression of host inflammatory and Candida albicans putative virulence factors was studied in women with vulvovaginal candidiasis (VVC; twenty) or colonized by the fungus but asymptomatic (carriers; fifteen) or non-colonized asymptomatic (ten subjects). Overexpression of genes encoding...

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Autores principales:	Elena Roselletti, Stefano Perito, Elena Gabrielli, Antonella Mencacci, Eva Pericolini, Samuele Sabbatini, Antonio Cassone, Anna Vecchiarelli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ffdfd47d53df4b639e35acb57d181a9e
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spelling	oai:doaj.org-article:ffdfd47d53df4b639e35acb57d181a9e2021-12-02T15:05:41ZNLRP3 inflammasome is a key player in human vulvovaginal disease caused by Candida albicans10.1038/s41598-017-17649-82045-2322https://doaj.org/article/ffdfd47d53df4b639e35acb57d181a9e2017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17649-8https://doaj.org/toc/2045-2322Abstract The expression of host inflammatory and Candida albicans putative virulence factors was studied in women with vulvovaginal candidiasis (VVC; twenty) or colonized by the fungus but asymptomatic (carriers; fifteen) or non-colonized asymptomatic (ten subjects). Overexpression of genes encoding NLRP3 and caspase-1 inflammasome components sharply differentiated VVC patients from asymptomatic colonized or non-colonized women. Inflammasome expression was coupled with neutrophils recruitment in the vagina of VVC women and IL-1β and IL-8 production. Both cytokines were present, though to a lower concentration, also in the vaginal fluid of colonized and non-colonized women. Secretory aspartyl proteinases (SAPs) and hyphae associated genes HWP1 and ECE1 were upregulated in VVC but with some differences among infected women. The most overexpressed SAP gene was SAP2, that correlated with neutrophils accumulation. Our data provide clinical evidence that the intracytoplasmic activation of NLRP3 inflammasome complex plays a critical, pathogenesis-relevant role in human VVC.Elena RosellettiStefano PeritoElena GabrielliAntonella MencacciEva PericoliniSamuele SabbatiniAntonio CassoneAnna VecchiarelliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elena Roselletti Stefano Perito Elena Gabrielli Antonella Mencacci Eva Pericolini Samuele Sabbatini Antonio Cassone Anna Vecchiarelli NLRP3 inflammasome is a key player in human vulvovaginal disease caused by Candida albicans
description	Abstract The expression of host inflammatory and Candida albicans putative virulence factors was studied in women with vulvovaginal candidiasis (VVC; twenty) or colonized by the fungus but asymptomatic (carriers; fifteen) or non-colonized asymptomatic (ten subjects). Overexpression of genes encoding NLRP3 and caspase-1 inflammasome components sharply differentiated VVC patients from asymptomatic colonized or non-colonized women. Inflammasome expression was coupled with neutrophils recruitment in the vagina of VVC women and IL-1β and IL-8 production. Both cytokines were present, though to a lower concentration, also in the vaginal fluid of colonized and non-colonized women. Secretory aspartyl proteinases (SAPs) and hyphae associated genes HWP1 and ECE1 were upregulated in VVC but with some differences among infected women. The most overexpressed SAP gene was SAP2, that correlated with neutrophils accumulation. Our data provide clinical evidence that the intracytoplasmic activation of NLRP3 inflammasome complex plays a critical, pathogenesis-relevant role in human VVC.
format	article
author	Elena Roselletti Stefano Perito Elena Gabrielli Antonella Mencacci Eva Pericolini Samuele Sabbatini Antonio Cassone Anna Vecchiarelli
author_facet	Elena Roselletti Stefano Perito Elena Gabrielli Antonella Mencacci Eva Pericolini Samuele Sabbatini Antonio Cassone Anna Vecchiarelli
author_sort	Elena Roselletti
title	NLRP3 inflammasome is a key player in human vulvovaginal disease caused by Candida albicans
title_short	NLRP3 inflammasome is a key player in human vulvovaginal disease caused by Candida albicans
title_full	NLRP3 inflammasome is a key player in human vulvovaginal disease caused by Candida albicans
title_fullStr	NLRP3 inflammasome is a key player in human vulvovaginal disease caused by Candida albicans
title_full_unstemmed	NLRP3 inflammasome is a key player in human vulvovaginal disease caused by Candida albicans
title_sort	nlrp3 inflammasome is a key player in human vulvovaginal disease caused by candida albicans
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ffdfd47d53df4b639e35acb57d181a9e
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NLRP3 inflammasome is a key player in human vulvovaginal disease caused by Candida albicans

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