Expression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment

Masoumeh Falah,1,2 Mohammad Najafi,2 Massoud Houshmand,3 Mohammad Farhadi1 1ENT and Head & Neck Research Center and Department, Iran University of Medical Sciences, Tehran, Iran; 2Cellular and Molecular Research Center, Biochemistry Department, Iran University of Medical Sciences, Tehran, I...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Falah M, Najafi M, Houshm, M, Farhadi M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Age related hearing impairment (ARHI) Presbycusis Biomarker Treatment. Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/ffe87a3ed7d5411ba488e3ed45d47fac
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ffe87a3ed7d5411ba488e3ed45d47fac
record_format	dspace
spelling	oai:doaj.org-article:ffe87a3ed7d5411ba488e3ed45d47fac2021-12-02T00:55:10ZExpression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment1178-1998https://doaj.org/article/ffe87a3ed7d5411ba488e3ed45d47fac2016-07-01T00:00:00Zhttps://www.dovepress.com/expression-levels-of-the-bak1-and-bcl2-genes-highlight-the-role-of-apo-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Masoumeh Falah,1,2 Mohammad Najafi,2 Massoud Houshmand,3 Mohammad Farhadi1 1ENT and Head & Neck Research Center and Department, Iran University of Medical Sciences, Tehran, Iran; 2Cellular and Molecular Research Center, Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran; 3Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran Abstract: Age-related hearing impairment (ARHI) is a progressive and a common sensory disorder in the elderly and will become an increasingly important clinical problem given the growing elderly population. Apoptosis of cochlear cells is an important factor in animal models of ARHI. As these cells cannot regenerate, their loss leads to irreversible hearing impairment. Identification of molecular mechanisms can facilitate disease prevention and effective treatment. In this study, we compared the expression of the genes BAK1 and BCL2 as two arms of the intrinsic apoptosis pathway between patients with ARHI and healthy subjects. ARHI and healthy subjects were selected after an ear nose throat examination, otoscopic investigation, and pure tone audiometry. RNA was extracted from peripheral blood samples, and relative gene expression levels were measured using quantitative real-time polymerase chain reaction. BAK1 and the BAK1/BCL2 ratio were statistically significantly upregulated in the ARHI subjects. The BAK1/BCL2 ratio was positively correlated with the results of the audiometric tests. Our results indicate that BAK-mediated apoptosis may be a core mechanism in the progression of ARHI in humans, similar to finding in animal models. Moreover, the gene expression changes in peripheral blood samples could be used as a rapid and simple biomarker for early detection of ARHI. Keywords: age-related hearing impairment (ARHI), presbycusis, biomarker, treatmentFalah MNajafi MHoushmMFarhadi MDove Medical PressarticleAge related hearing impairment (ARHI)PresbycusisBiomarkerTreatment.GeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 11, Pp 1003-1008 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Age related hearing impairment (ARHI) Presbycusis Biomarker Treatment. Geriatrics RC952-954.6
spellingShingle	Age related hearing impairment (ARHI) Presbycusis Biomarker Treatment. Geriatrics RC952-954.6 Falah M Najafi M Houshm M Farhadi M Expression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment
description	Masoumeh Falah,1,2 Mohammad Najafi,2 Massoud Houshmand,3 Mohammad Farhadi1 1ENT and Head & Neck Research Center and Department, Iran University of Medical Sciences, Tehran, Iran; 2Cellular and Molecular Research Center, Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran; 3Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran Abstract: Age-related hearing impairment (ARHI) is a progressive and a common sensory disorder in the elderly and will become an increasingly important clinical problem given the growing elderly population. Apoptosis of cochlear cells is an important factor in animal models of ARHI. As these cells cannot regenerate, their loss leads to irreversible hearing impairment. Identification of molecular mechanisms can facilitate disease prevention and effective treatment. In this study, we compared the expression of the genes BAK1 and BCL2 as two arms of the intrinsic apoptosis pathway between patients with ARHI and healthy subjects. ARHI and healthy subjects were selected after an ear nose throat examination, otoscopic investigation, and pure tone audiometry. RNA was extracted from peripheral blood samples, and relative gene expression levels were measured using quantitative real-time polymerase chain reaction. BAK1 and the BAK1/BCL2 ratio were statistically significantly upregulated in the ARHI subjects. The BAK1/BCL2 ratio was positively correlated with the results of the audiometric tests. Our results indicate that BAK-mediated apoptosis may be a core mechanism in the progression of ARHI in humans, similar to finding in animal models. Moreover, the gene expression changes in peripheral blood samples could be used as a rapid and simple biomarker for early detection of ARHI. Keywords: age-related hearing impairment (ARHI), presbycusis, biomarker, treatment
format	article
author	Falah M Najafi M Houshm M Farhadi M
author_facet	Falah M Najafi M Houshm M Farhadi M
author_sort	Falah M
title	Expression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment
title_short	Expression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment
title_full	Expression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment
title_fullStr	Expression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment
title_full_unstemmed	Expression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment
title_sort	expression levels of the bak1 and bcl2 genes highlight the role of apoptosis in age-related hearing impairment
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/ffe87a3ed7d5411ba488e3ed45d47fac
work_keys_str_mv	AT falahm expressionlevelsofthebak1andbcl2geneshighlighttheroleofapoptosisinagerelatedhearingimpairment AT najafim expressionlevelsofthebak1andbcl2geneshighlighttheroleofapoptosisinagerelatedhearingimpairment AT houshm expressionlevelsofthebak1andbcl2geneshighlighttheroleofapoptosisinagerelatedhearingimpairment AT m expressionlevelsofthebak1andbcl2geneshighlighttheroleofapoptosisinagerelatedhearingimpairment AT farhadim expressionlevelsofthebak1andbcl2geneshighlighttheroleofapoptosisinagerelatedhearingimpairment
_version_	1718403415089872896

Expression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment

Ejemplares similares