LIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U937

Treatment of malignant tumors is among challenging issues of clinical medicine. Dissemination and matestasis of tumor cells plays a major role in oncology, being immediately dependent on chemotaxis of tumor cells. Hence, the aim of our study is to evaluate migration of tumor cells, in terms of ligan...

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Autores principales:	A. B. Filina, O. A. Svitich, L. V. Gankovskaya, P. A. Labzhinov, T. M. Parfenova, V. V. Zverev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	chemotaxis tumor cells u937 line tlrs chemokines boyden chamber assay Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ffeb04e6891348aaa848f850d086006c
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spelling	oai:doaj.org-article:ffeb04e6891348aaa848f850d086006c2021-11-18T08:03:44ZLIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U9371563-06252313-741X10.15789/1563-0625-2014-5-443-448https://doaj.org/article/ffeb04e6891348aaa848f850d086006c2014-11-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/731https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XTreatment of malignant tumors is among challenging issues of clinical medicine. Dissemination and matestasis of tumor cells plays a major role in oncology, being immediately dependent on chemotaxis of tumor cells. Hence, the aim of our study is to evaluate migration of tumor cells, in terms of ligand-mediated chemotaxis of a human U 937 lymphoma cell line. Synthetic TLR ligands (DNA_lig, RNA_lig) were used as chemoattractants. Assessment of time-dependent TLR expression by the migrating cells (including TLR2, TLR3, TLR7, TLR9) was carried out by means of real-time PCR, using Boyden’s chamber system for the migration assays. We have revealed an increased cell migration towards DNA_ lig and TNFα gradient. Expression of TLR2, TLR7 and TLR9 was found to be increased under the influence of TNFα (respectively 1.5-, 4- and 19-fold). Under the influence of DNA_lig, TLR9 expression was 105-fold increased, whereas TLR3 expression was 2-fold higher, along with decrease of TLR2 expression. Expression of TLR 3 was shown to be 3-times higher under the influence of RNA_lig. The effects observed could be potentially applied for suppression of tumor cell chemotaxis by means of these ligands and by influencing different pathways ofchemotaxis regulation.A. B. FilinaO. A. SvitichL. V. GankovskayaP. A. LabzhinovT. M. ParfenovaV. V. ZverevSPb RAACIarticlechemotaxistumor cellsu937 linetlrschemokinesboyden chamber assayImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 16, Iss 5, Pp 443-448 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	chemotaxis tumor cells u937 line tlrs chemokines boyden chamber assay Immunologic diseases. Allergy RC581-607
spellingShingle	chemotaxis tumor cells u937 line tlrs chemokines boyden chamber assay Immunologic diseases. Allergy RC581-607 A. B. Filina O. A. Svitich L. V. Gankovskaya P. A. Labzhinov T. M. Parfenova V. V. Zverev LIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U937
description	Treatment of malignant tumors is among challenging issues of clinical medicine. Dissemination and matestasis of tumor cells plays a major role in oncology, being immediately dependent on chemotaxis of tumor cells. Hence, the aim of our study is to evaluate migration of tumor cells, in terms of ligand-mediated chemotaxis of a human U 937 lymphoma cell line. Synthetic TLR ligands (DNA_lig, RNA_lig) were used as chemoattractants. Assessment of time-dependent TLR expression by the migrating cells (including TLR2, TLR3, TLR7, TLR9) was carried out by means of real-time PCR, using Boyden’s chamber system for the migration assays. We have revealed an increased cell migration towards DNA_ lig and TNFα gradient. Expression of TLR2, TLR7 and TLR9 was found to be increased under the influence of TNFα (respectively 1.5-, 4- and 19-fold). Under the influence of DNA_lig, TLR9 expression was 105-fold increased, whereas TLR3 expression was 2-fold higher, along with decrease of TLR2 expression. Expression of TLR 3 was shown to be 3-times higher under the influence of RNA_lig. The effects observed could be potentially applied for suppression of tumor cell chemotaxis by means of these ligands and by influencing different pathways ofchemotaxis regulation.
format	article
author	A. B. Filina O. A. Svitich L. V. Gankovskaya P. A. Labzhinov T. M. Parfenova V. V. Zverev
author_facet	A. B. Filina O. A. Svitich L. V. Gankovskaya P. A. Labzhinov T. M. Parfenova V. V. Zverev
author_sort	A. B. Filina
title	LIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U937
title_short	LIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U937
title_full	LIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U937
title_fullStr	LIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U937
title_full_unstemmed	LIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U937
title_sort	ligand induced chemotaxis of macrophage cell line u937
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/ffeb04e6891348aaa848f850d086006c
work_keys_str_mv	AT abfilina ligandinducedchemotaxisofmacrophagecelllineu937 AT oasvitich ligandinducedchemotaxisofmacrophagecelllineu937 AT lvgankovskaya ligandinducedchemotaxisofmacrophagecelllineu937 AT palabzhinov ligandinducedchemotaxisofmacrophagecelllineu937 AT tmparfenova ligandinducedchemotaxisofmacrophagecelllineu937 AT vvzverev ligandinducedchemotaxisofmacrophagecelllineu937
_version_	1718422458369835008

LIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U937

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