Epiphytic bet-mannanase producing bacterial strains

Dry remains of the herbal species of the plantain (Plantago major), the wormwood (Artemisia vulgaris) and the reed grass (Calamagrostis acutiflora) were used as a natural source for isolation of β- mannanase producing strains. They were isolated by using the carob gum as a single source of carbon an...

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Autores principales:	Epova Ekaterina, Petrova Svetlana, Trubnikova Elena, Danilova Maria
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/ffee4a5b618e4e23aceeed1214bf4d72
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spelling	oai:doaj.org-article:ffee4a5b618e4e23aceeed1214bf4d722021-11-08T15:17:24ZEpiphytic bet-mannanase producing bacterial strains2117-445810.1051/bioconf/20213700125https://doaj.org/article/ffee4a5b618e4e23aceeed1214bf4d722021-01-01T00:00:00Zhttps://www.bio-conferences.org/articles/bioconf/pdf/2021/09/bioconf_fies2021_00125.pdfhttps://doaj.org/toc/2117-4458Dry remains of the herbal species of the plantain (Plantago major), the wormwood (Artemisia vulgaris) and the reed grass (Calamagrostis acutiflora) were used as a natural source for isolation of β- mannanase producing strains. They were isolated by using the carob gum as a single source of carbon and energy. Each chosen plant species was found to be colonized with a single dominant epiphytic group of microorganism, although the plants had been collected in the same location. Bacillus circulans was only found in P. major, Bacillus subtilis on A. vulgaris, whereas Pantoea sp. was found in C. acutiflora. Identification of the taxonomy affiliation of the isolated β-mannanase producers allowed using the formerly proposed primers for PCR cloning of β-mannanase genes previously occurred in the respective bacterial species. This approach let us cloning 330 bp fragment of β-mannanase genes from B. circulans and B. subtilis and 1000 bp fragment of β-mannanase gene from Pantoea sp. Testing the enzymatic activity of the isolated strains by staining the carob gum hydrolysis zones on the plates with Congo Red was carried out. As a result, the maximum activity was found in Pantoea sp.Epova EkaterinaPetrova SvetlanaTrubnikova ElenaDanilova MariaEDP SciencesarticleMicrobiologyQR1-502PhysiologyQP1-981ZoologyQL1-991ENFRBIO Web of Conferences, Vol 37, p 00125 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
spellingShingle	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 Epova Ekaterina Petrova Svetlana Trubnikova Elena Danilova Maria Epiphytic bet-mannanase producing bacterial strains
description	Dry remains of the herbal species of the plantain (Plantago major), the wormwood (Artemisia vulgaris) and the reed grass (Calamagrostis acutiflora) were used as a natural source for isolation of β- mannanase producing strains. They were isolated by using the carob gum as a single source of carbon and energy. Each chosen plant species was found to be colonized with a single dominant epiphytic group of microorganism, although the plants had been collected in the same location. Bacillus circulans was only found in P. major, Bacillus subtilis on A. vulgaris, whereas Pantoea sp. was found in C. acutiflora. Identification of the taxonomy affiliation of the isolated β-mannanase producers allowed using the formerly proposed primers for PCR cloning of β-mannanase genes previously occurred in the respective bacterial species. This approach let us cloning 330 bp fragment of β-mannanase genes from B. circulans and B. subtilis and 1000 bp fragment of β-mannanase gene from Pantoea sp. Testing the enzymatic activity of the isolated strains by staining the carob gum hydrolysis zones on the plates with Congo Red was carried out. As a result, the maximum activity was found in Pantoea sp.
format	article
author	Epova Ekaterina Petrova Svetlana Trubnikova Elena Danilova Maria
author_facet	Epova Ekaterina Petrova Svetlana Trubnikova Elena Danilova Maria
author_sort	Epova Ekaterina
title	Epiphytic bet-mannanase producing bacterial strains
title_short	Epiphytic bet-mannanase producing bacterial strains
title_full	Epiphytic bet-mannanase producing bacterial strains
title_fullStr	Epiphytic bet-mannanase producing bacterial strains
title_full_unstemmed	Epiphytic bet-mannanase producing bacterial strains
title_sort	epiphytic bet-mannanase producing bacterial strains
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/ffee4a5b618e4e23aceeed1214bf4d72
work_keys_str_mv	AT epovaekaterina epiphyticbetmannanaseproducingbacterialstrains AT petrovasvetlana epiphyticbetmannanaseproducingbacterialstrains AT trubnikovaelena epiphyticbetmannanaseproducingbacterialstrains AT danilovamaria epiphyticbetmannanaseproducingbacterialstrains
_version_	1718442105885425664

Epiphytic bet-mannanase producing bacterial strains

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