Impact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation

Abstract Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen that forms robust biofilms in the different niches it occupies. Numerous physiological adaptations are required as this organism shifts from soil or aquatic environments to a host-associated lifestyle. While many conditions diffe...

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Autores principales:	Karishma Bisht, Jessica L. Moore, Richard M. Caprioli, Eric P. Skaar, Catherine A. Wakeman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/ffefceea8e694d5e9a0738aa433e3004
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spelling	oai:doaj.org-article:ffefceea8e694d5e9a0738aa433e30042021-12-02T17:05:47ZImpact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation10.1038/s41522-021-00194-82055-5008https://doaj.org/article/ffefceea8e694d5e9a0738aa433e30042021-03-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00194-8https://doaj.org/toc/2055-5008Abstract Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen that forms robust biofilms in the different niches it occupies. Numerous physiological adaptations are required as this organism shifts from soil or aquatic environments to a host-associated lifestyle. While many conditions differ between these niches, temperature shifts are a factor that can contribute to physiological stress during this transition. To understand how temperature impacts biofilm formation in this pathogen, we used proteomic and transcriptomic tools to elucidate physiological responses in environment-relevant vs. host-relevant temperatures. These studies uncovered differential expression of various proteins including a phage protein that is associated with the EPS matrix in P. aeruginosa. This filamentous phage was induced at host temperatures and was required for full biofilm-forming capacity specifically at human body temperature. These data highlight the importance of temperature shift in biofilm formation and suggest bacteriophage proteins could be a possible therapeutic target in biofilm-associated infections.Karishma BishtJessica L. MooreRichard M. CaprioliEric P. SkaarCatherine A. WakemanNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Karishma Bisht Jessica L. Moore Richard M. Caprioli Eric P. Skaar Catherine A. Wakeman Impact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation
description	Abstract Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen that forms robust biofilms in the different niches it occupies. Numerous physiological adaptations are required as this organism shifts from soil or aquatic environments to a host-associated lifestyle. While many conditions differ between these niches, temperature shifts are a factor that can contribute to physiological stress during this transition. To understand how temperature impacts biofilm formation in this pathogen, we used proteomic and transcriptomic tools to elucidate physiological responses in environment-relevant vs. host-relevant temperatures. These studies uncovered differential expression of various proteins including a phage protein that is associated with the EPS matrix in P. aeruginosa. This filamentous phage was induced at host temperatures and was required for full biofilm-forming capacity specifically at human body temperature. These data highlight the importance of temperature shift in biofilm formation and suggest bacteriophage proteins could be a possible therapeutic target in biofilm-associated infections.
format	article
author	Karishma Bisht Jessica L. Moore Richard M. Caprioli Eric P. Skaar Catherine A. Wakeman
author_facet	Karishma Bisht Jessica L. Moore Richard M. Caprioli Eric P. Skaar Catherine A. Wakeman
author_sort	Karishma Bisht
title	Impact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation
title_short	Impact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation
title_full	Impact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation
title_fullStr	Impact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation
title_full_unstemmed	Impact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation
title_sort	impact of temperature-dependent phage expression on pseudomonas aeruginosa biofilm formation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ffefceea8e694d5e9a0738aa433e3004
work_keys_str_mv	AT karishmabisht impactoftemperaturedependentphageexpressiononpseudomonasaeruginosabiofilmformation AT jessicalmoore impactoftemperaturedependentphageexpressiononpseudomonasaeruginosabiofilmformation AT richardmcaprioli impactoftemperaturedependentphageexpressiononpseudomonasaeruginosabiofilmformation AT ericpskaar impactoftemperaturedependentphageexpressiononpseudomonasaeruginosabiofilmformation AT catherineawakeman impactoftemperaturedependentphageexpressiononpseudomonasaeruginosabiofilmformation
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Impact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation

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