Neuroprotective effects of long noncoding RNAs involved in ischemic postconditioning after ischemic stroke

During acute reperfusion, the expression profiles of long noncoding RNAs in adult rats with focal cerebral ischemia undergo broad changes. However, whether long noncoding RNAs are involved in neuroprotective effects following focal ischemic stroke in rats remains unclear. In this study, RNA isolatio...

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Autores principales:	Wei Ma, Chun-Yan Li, Si-Jia Zhang, Cheng-Hao Zang, Jin-Wei Yang, Zhen Wu, Guo-Dong Wang, Jie Liu, Wei Liu, Kuang-Pin Liu, Yu Liang, Xing-Kui Zhang, Jun-Jun Li, Jian-Hui Guo, Li-Yan Li
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2022
Materias:	cerebral infarction; differential expression analysis; expression profiling; go term; ischemic postconditioning; ischemic stroke; kegg pathway; lncrna; mrna; rna sequencing Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/fff115d1a7b54330897669322ae3fe0f
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spelling	oai:doaj.org-article:fff115d1a7b54330897669322ae3fe0f2021-11-19T12:16:44ZNeuroprotective effects of long noncoding RNAs involved in ischemic postconditioning after ischemic stroke1673-537410.4103/1673-5374.327346https://doaj.org/article/fff115d1a7b54330897669322ae3fe0f2022-01-01T00:00:00Zhttp://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=6;spage=1299;epage=1309;aulast=Mahttps://doaj.org/toc/1673-5374During acute reperfusion, the expression profiles of long noncoding RNAs in adult rats with focal cerebral ischemia undergo broad changes. However, whether long noncoding RNAs are involved in neuroprotective effects following focal ischemic stroke in rats remains unclear. In this study, RNA isolation and library preparation was performed for long noncoding RNA sequencing, followed by determining the coding potential of identified long noncoding RNAs and target gene prediction. Differential expression analysis, long noncoding RNA functional enrichment analysis, and co-expression network analysis were performed comparing ischemic rats with and without ischemic postconditioning rats. Rats were subjected to ischemic postconditioning via the brief and repeated occlusion of the middle cerebral artery or femoral artery. Quantitative real-time reverse transcription-polymerase chain reaction was used to detect the expression levels of differentially expressed long noncoding RNAs after ischemic postconditioning in a rat model of ischemic stroke. The results showed that ischemic postconditioning greatly affected the expression profile of long noncoding RNAs and mRNAs in the brains of rats that underwent ischemic stroke. The predicted target genes of some of the identified long noncoding RNAs (cis targets) were related to the cellular response to ischemia and stress, cytokine signal transduction, inflammation, and apoptosis signal transduction pathways. In addition, 15 significantly differentially expressed long noncoding RNAs were identified in the brains of rats subjected to ischemic postconditioning. Nine candidate long noncoding RNAs that may be related to ischemic postconditioning were identified by a long noncoding RNA expression profile and long noncoding RNA-mRNA co-expression network analysis. Expression levels were verified by quantitative real-time reverse transcription-polymerase chain reaction. These results suggested that the identified long noncoding RNAs may be involved in the neuroprotective effects associated with ischemic postconditioning following ischemic stroke. The experimental animal procedures were approved by the Animal Experiment Ethics Committee of Kunming Medical University (approval No. KMMU2018018) in January 2018.Wei MaChun-Yan LiSi-Jia ZhangCheng-Hao ZangJin-Wei YangZhen WuGuo-Dong WangJie LiuWei LiuKuang-Pin LiuYu LiangXing-Kui ZhangJun-Jun LiJian-Hui GuoLi-Yan LiWolters Kluwer Medknow Publicationsarticlecerebral infarction; differential expression analysis; expression profiling; go term; ischemic postconditioning; ischemic stroke; kegg pathway; lncrna; mrna; rna sequencingNeurology. Diseases of the nervous systemRC346-429ENNeural Regeneration Research, Vol 17, Iss 6, Pp 1299-1309 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	cerebral infarction; differential expression analysis; expression profiling; go term; ischemic postconditioning; ischemic stroke; kegg pathway; lncrna; mrna; rna sequencing Neurology. Diseases of the nervous system RC346-429
spellingShingle	cerebral infarction; differential expression analysis; expression profiling; go term; ischemic postconditioning; ischemic stroke; kegg pathway; lncrna; mrna; rna sequencing Neurology. Diseases of the nervous system RC346-429 Wei Ma Chun-Yan Li Si-Jia Zhang Cheng-Hao Zang Jin-Wei Yang Zhen Wu Guo-Dong Wang Jie Liu Wei Liu Kuang-Pin Liu Yu Liang Xing-Kui Zhang Jun-Jun Li Jian-Hui Guo Li-Yan Li Neuroprotective effects of long noncoding RNAs involved in ischemic postconditioning after ischemic stroke
description	During acute reperfusion, the expression profiles of long noncoding RNAs in adult rats with focal cerebral ischemia undergo broad changes. However, whether long noncoding RNAs are involved in neuroprotective effects following focal ischemic stroke in rats remains unclear. In this study, RNA isolation and library preparation was performed for long noncoding RNA sequencing, followed by determining the coding potential of identified long noncoding RNAs and target gene prediction. Differential expression analysis, long noncoding RNA functional enrichment analysis, and co-expression network analysis were performed comparing ischemic rats with and without ischemic postconditioning rats. Rats were subjected to ischemic postconditioning via the brief and repeated occlusion of the middle cerebral artery or femoral artery. Quantitative real-time reverse transcription-polymerase chain reaction was used to detect the expression levels of differentially expressed long noncoding RNAs after ischemic postconditioning in a rat model of ischemic stroke. The results showed that ischemic postconditioning greatly affected the expression profile of long noncoding RNAs and mRNAs in the brains of rats that underwent ischemic stroke. The predicted target genes of some of the identified long noncoding RNAs (cis targets) were related to the cellular response to ischemia and stress, cytokine signal transduction, inflammation, and apoptosis signal transduction pathways. In addition, 15 significantly differentially expressed long noncoding RNAs were identified in the brains of rats subjected to ischemic postconditioning. Nine candidate long noncoding RNAs that may be related to ischemic postconditioning were identified by a long noncoding RNA expression profile and long noncoding RNA-mRNA co-expression network analysis. Expression levels were verified by quantitative real-time reverse transcription-polymerase chain reaction. These results suggested that the identified long noncoding RNAs may be involved in the neuroprotective effects associated with ischemic postconditioning following ischemic stroke. The experimental animal procedures were approved by the Animal Experiment Ethics Committee of Kunming Medical University (approval No. KMMU2018018) in January 2018.
format	article
author	Wei Ma Chun-Yan Li Si-Jia Zhang Cheng-Hao Zang Jin-Wei Yang Zhen Wu Guo-Dong Wang Jie Liu Wei Liu Kuang-Pin Liu Yu Liang Xing-Kui Zhang Jun-Jun Li Jian-Hui Guo Li-Yan Li
author_facet	Wei Ma Chun-Yan Li Si-Jia Zhang Cheng-Hao Zang Jin-Wei Yang Zhen Wu Guo-Dong Wang Jie Liu Wei Liu Kuang-Pin Liu Yu Liang Xing-Kui Zhang Jun-Jun Li Jian-Hui Guo Li-Yan Li
author_sort	Wei Ma
title	Neuroprotective effects of long noncoding RNAs involved in ischemic postconditioning after ischemic stroke
title_short	Neuroprotective effects of long noncoding RNAs involved in ischemic postconditioning after ischemic stroke
title_full	Neuroprotective effects of long noncoding RNAs involved in ischemic postconditioning after ischemic stroke
title_fullStr	Neuroprotective effects of long noncoding RNAs involved in ischemic postconditioning after ischemic stroke
title_full_unstemmed	Neuroprotective effects of long noncoding RNAs involved in ischemic postconditioning after ischemic stroke
title_sort	neuroprotective effects of long noncoding rnas involved in ischemic postconditioning after ischemic stroke
publisher	Wolters Kluwer Medknow Publications
publishDate	2022
url	https://doaj.org/article/fff115d1a7b54330897669322ae3fe0f
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Neuroprotective effects of long noncoding RNAs involved in ischemic postconditioning after ischemic stroke

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