Hormonal regulation of ovarian bursa fluid in mice and involvement of aquaporins.
In rodent species, the ovary and the end of oviduct are encapsulated by a thin membrane called ovarian bursa. The biological functions of ovarian bursa remain unexplored despite its structural arrangement in facilitating oocytes transport into oviduct. In the present study, we observed a rapid fluid...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/fffa3fa82a6a47e688164736cb68bbd4 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:fffa3fa82a6a47e688164736cb68bbd4 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:fffa3fa82a6a47e688164736cb68bbd42021-11-18T07:44:40ZHormonal regulation of ovarian bursa fluid in mice and involvement of aquaporins.1932-620310.1371/journal.pone.0063823https://doaj.org/article/fffa3fa82a6a47e688164736cb68bbd42013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23717491/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In rodent species, the ovary and the end of oviduct are encapsulated by a thin membrane called ovarian bursa. The biological functions of ovarian bursa remain unexplored despite its structural arrangement in facilitating oocytes transport into oviduct. In the present study, we observed a rapid fluid accumulation and reabsorption within the ovarian bursa after ovarian stimulation (PMSG-primed hCG injection), suggesting that the ovarian bursa might play an active role in regulating local fluid homeostasis around the timing of ovulation. We hypothesized that the aquaporin proteins, which are specialized channels for water transport, might be involved in this process. By screening the expression of aquaporin family members (Aqp1-9) in the ovarian tissue and isolated ovarian bursa (0, 1, 2 and 5 h after hCG injection), we found that AQP2 and AQP5 mRNA showed dynamic changes after hCG treatment, showing upregulation at 1-2 h followed by gradually decrease at 5 h, which is closely related with the intra-bursa fluid dynamics. Further immunofluorescence examinations of AQP2 and AQP5 in the ovarian bursa revealed that AQP2 is specifically localized in the outer layer (peritoneal side) while AQP5 localized in the inner layer (ovarian side) of the bursa, such cell type specific and spatial-temporal expressions of AQP2 and 5 support our hypothesis that they might be involved in efficient water transport through ovarian bursa under ovulation related hormonal regulation. The physiological significance of aquaporin-mediated water transport in the context of ovarian bursa still awaits further clarification.He ZhangYing ZhangHuashan ZhaoYunfang ZhangQi ChenHongying PengLi LeiJingqiao QiaoJunchao ShiZhonghong CaoEnkui DuanYaping JinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63823 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q He Zhang Ying Zhang Huashan Zhao Yunfang Zhang Qi Chen Hongying Peng Li Lei Jingqiao Qiao Junchao Shi Zhonghong Cao Enkui Duan Yaping Jin Hormonal regulation of ovarian bursa fluid in mice and involvement of aquaporins. |
| description |
In rodent species, the ovary and the end of oviduct are encapsulated by a thin membrane called ovarian bursa. The biological functions of ovarian bursa remain unexplored despite its structural arrangement in facilitating oocytes transport into oviduct. In the present study, we observed a rapid fluid accumulation and reabsorption within the ovarian bursa after ovarian stimulation (PMSG-primed hCG injection), suggesting that the ovarian bursa might play an active role in regulating local fluid homeostasis around the timing of ovulation. We hypothesized that the aquaporin proteins, which are specialized channels for water transport, might be involved in this process. By screening the expression of aquaporin family members (Aqp1-9) in the ovarian tissue and isolated ovarian bursa (0, 1, 2 and 5 h after hCG injection), we found that AQP2 and AQP5 mRNA showed dynamic changes after hCG treatment, showing upregulation at 1-2 h followed by gradually decrease at 5 h, which is closely related with the intra-bursa fluid dynamics. Further immunofluorescence examinations of AQP2 and AQP5 in the ovarian bursa revealed that AQP2 is specifically localized in the outer layer (peritoneal side) while AQP5 localized in the inner layer (ovarian side) of the bursa, such cell type specific and spatial-temporal expressions of AQP2 and 5 support our hypothesis that they might be involved in efficient water transport through ovarian bursa under ovulation related hormonal regulation. The physiological significance of aquaporin-mediated water transport in the context of ovarian bursa still awaits further clarification. |
| format |
article |
| author |
He Zhang Ying Zhang Huashan Zhao Yunfang Zhang Qi Chen Hongying Peng Li Lei Jingqiao Qiao Junchao Shi Zhonghong Cao Enkui Duan Yaping Jin |
| author_facet |
He Zhang Ying Zhang Huashan Zhao Yunfang Zhang Qi Chen Hongying Peng Li Lei Jingqiao Qiao Junchao Shi Zhonghong Cao Enkui Duan Yaping Jin |
| author_sort |
He Zhang |
| title |
Hormonal regulation of ovarian bursa fluid in mice and involvement of aquaporins. |
| title_short |
Hormonal regulation of ovarian bursa fluid in mice and involvement of aquaporins. |
| title_full |
Hormonal regulation of ovarian bursa fluid in mice and involvement of aquaporins. |
| title_fullStr |
Hormonal regulation of ovarian bursa fluid in mice and involvement of aquaporins. |
| title_full_unstemmed |
Hormonal regulation of ovarian bursa fluid in mice and involvement of aquaporins. |
| title_sort |
hormonal regulation of ovarian bursa fluid in mice and involvement of aquaporins. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/fffa3fa82a6a47e688164736cb68bbd4 |
| work_keys_str_mv |
AT hezhang hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT yingzhang hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT huashanzhao hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT yunfangzhang hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT qichen hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT hongyingpeng hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT lilei hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT jingqiaoqiao hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT junchaoshi hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT zhonghongcao hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT enkuiduan hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins AT yapingjin hormonalregulationofovarianbursafluidinmiceandinvolvementofaquaporins |
| _version_ |
1718423075379216384 |