Scrapie Resistance Gene Identification using Optimized Taqman Test qPCR Method in Sheep on the Territory of the Republic of Serbia

Scrapie is an infectious neurodegenerative disease affecting the central nervous system of sheep and goats that belongs to transmissible spongiform encephalopathies. The disease is caused by the accumulation of proteinase-resistant isoform of the prion protein. The sheep predisposition to scrapie is...

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Autores principales:	Nešić Slađan, Jelisić Stefan, Aleksić-Kovačević Sanja, Aničić Milan, Vučićević Ivana
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	genotyping polymorphisms qpcr scrapie taqman probes Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/fffae22494ef49c7bfb1640f2c076320
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spelling	oai:doaj.org-article:fffae22494ef49c7bfb1640f2c0763202021-11-17T21:27:54ZScrapie Resistance Gene Identification using Optimized Taqman Test qPCR Method in Sheep on the Territory of the Republic of Serbia1820-744810.2478/acve-2021-0016https://doaj.org/article/fffae22494ef49c7bfb1640f2c0763202021-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2021-0016https://doaj.org/toc/1820-7448Scrapie is an infectious neurodegenerative disease affecting the central nervous system of sheep and goats that belongs to transmissible spongiform encephalopathies. The disease is caused by the accumulation of proteinase-resistant isoform of the prion protein. The sheep predisposition to scrapie is associated with polymorphisms of the PrP gene. Genetic susceptibility to scrapie is mainly related to codons 136, 154, and 171. ARR sheep are strongly scrapie resistant and VRQ genotype is the most susceptible. Many countries have scrapie eradication programs based on using rams with resistant genotype. The eradication program has not yet been implemented in the Republic of Serbia. To examine the genetic makeup of sheep in Serbia related to scrapie, we optimized TaqMan probes of real-time polymerase chain reaction (qPCR) technique for three codons. Blood samples from 100 sheep were analyzed by qPCR and the majority of the examined sheep were AA homozygous for the 136 codon. For codon 154 the most frequent genotype was RR and for codon 171 the most frequent genotype was QQ.Nešić SlađanJelisić StefanAleksić-Kovačević SanjaAničić MilanVučićević IvanaSciendoarticlegenotypingpolymorphismsqpcrscrapietaqman probesVeterinary medicineSF600-1100ENActa Veterinaria, Vol 71, Iss 2, Pp 189-197 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	genotyping polymorphisms qpcr scrapie taqman probes Veterinary medicine SF600-1100
spellingShingle	genotyping polymorphisms qpcr scrapie taqman probes Veterinary medicine SF600-1100 Nešić Slađan Jelisić Stefan Aleksić-Kovačević Sanja Aničić Milan Vučićević Ivana Scrapie Resistance Gene Identification using Optimized Taqman Test qPCR Method in Sheep on the Territory of the Republic of Serbia
description	Scrapie is an infectious neurodegenerative disease affecting the central nervous system of sheep and goats that belongs to transmissible spongiform encephalopathies. The disease is caused by the accumulation of proteinase-resistant isoform of the prion protein. The sheep predisposition to scrapie is associated with polymorphisms of the PrP gene. Genetic susceptibility to scrapie is mainly related to codons 136, 154, and 171. ARR sheep are strongly scrapie resistant and VRQ genotype is the most susceptible. Many countries have scrapie eradication programs based on using rams with resistant genotype. The eradication program has not yet been implemented in the Republic of Serbia. To examine the genetic makeup of sheep in Serbia related to scrapie, we optimized TaqMan probes of real-time polymerase chain reaction (qPCR) technique for three codons. Blood samples from 100 sheep were analyzed by qPCR and the majority of the examined sheep were AA homozygous for the 136 codon. For codon 154 the most frequent genotype was RR and for codon 171 the most frequent genotype was QQ.
format	article
author	Nešić Slađan Jelisić Stefan Aleksić-Kovačević Sanja Aničić Milan Vučićević Ivana
author_facet	Nešić Slađan Jelisić Stefan Aleksić-Kovačević Sanja Aničić Milan Vučićević Ivana
author_sort	Nešić Slađan
title	Scrapie Resistance Gene Identification using Optimized Taqman Test qPCR Method in Sheep on the Territory of the Republic of Serbia
title_short	Scrapie Resistance Gene Identification using Optimized Taqman Test qPCR Method in Sheep on the Territory of the Republic of Serbia
title_full	Scrapie Resistance Gene Identification using Optimized Taqman Test qPCR Method in Sheep on the Territory of the Republic of Serbia
title_fullStr	Scrapie Resistance Gene Identification using Optimized Taqman Test qPCR Method in Sheep on the Territory of the Republic of Serbia
title_full_unstemmed	Scrapie Resistance Gene Identification using Optimized Taqman Test qPCR Method in Sheep on the Territory of the Republic of Serbia
title_sort	scrapie resistance gene identification using optimized taqman test qpcr method in sheep on the territory of the republic of serbia
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/fffae22494ef49c7bfb1640f2c076320
work_keys_str_mv	AT nesicslađan scrapieresistancegeneidentificationusingoptimizedtaqmantestqpcrmethodinsheepontheterritoryoftherepublicofserbia AT jelisicstefan scrapieresistancegeneidentificationusingoptimizedtaqmantestqpcrmethodinsheepontheterritoryoftherepublicofserbia AT aleksickovacevicsanja scrapieresistancegeneidentificationusingoptimizedtaqmantestqpcrmethodinsheepontheterritoryoftherepublicofserbia AT anicicmilan scrapieresistancegeneidentificationusingoptimizedtaqmantestqpcrmethodinsheepontheterritoryoftherepublicofserbia AT vucicevicivana scrapieresistancegeneidentificationusingoptimizedtaqmantestqpcrmethodinsheepontheterritoryoftherepublicofserbia
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Scrapie Resistance Gene Identification using Optimized Taqman Test qPCR Method in Sheep on the Territory of the Republic of Serbia

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