An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components
Abstract During oviposition, ectoparasitoid wasps not only inject their eggs but also a complex mixture of proteins and peptides (venom) in order to regulate the host physiology to benefit their progeny. Although several endoparasitoid venom proteins have been identified, little is known about the c...
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2021
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oai:doaj.org-article:fffcd1ef123041afa08ff173ef12fcb72021-12-02T15:53:02ZAn integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components10.1038/s41598-021-84385-52045-2322https://doaj.org/article/fffcd1ef123041afa08ff173ef12fcb72021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84385-5https://doaj.org/toc/2045-2322Abstract During oviposition, ectoparasitoid wasps not only inject their eggs but also a complex mixture of proteins and peptides (venom) in order to regulate the host physiology to benefit their progeny. Although several endoparasitoid venom proteins have been identified, little is known about the components of ectoparasitoid venom. To characterize the protein composition of Torymus sinensis Kamijo (Hymenoptera: Torymidae) venom, we used an integrated transcriptomic and proteomic approach and identified 143 venom proteins. Moreover, focusing on venom gland transcriptome, we selected additional 52 transcripts encoding putative venom proteins. As in other parasitoid venoms, hydrolases, including proteases, phosphatases, esterases, and nucleases, constitute the most abundant families in T. sinensis venom, followed by protease inhibitors. These proteins are potentially involved in the complex parasitic syndrome, with different effects on the immune system, physiological processes and development of the host, and contribute to provide nutrients to the parasitoid progeny. Although additional in vivo studies are needed, initial findings offer important information about venom factors and their putative host effects, which are essential to ensure the success of parasitism.Carmen ScieuzoRosanna SalviaAntonio FrancoMarco PezziFlora CozzolinoMilvia ChiccaChiara ScapoliHeiko VogelMaria MontiChiara FerraciniPietro PucciAlberto AlmaPatrizia FalabellaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-25 (2021) |
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Medicine R Science Q Carmen Scieuzo Rosanna Salvia Antonio Franco Marco Pezzi Flora Cozzolino Milvia Chicca Chiara Scapoli Heiko Vogel Maria Monti Chiara Ferracini Pietro Pucci Alberto Alma Patrizia Falabella An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components |
| description |
Abstract During oviposition, ectoparasitoid wasps not only inject their eggs but also a complex mixture of proteins and peptides (venom) in order to regulate the host physiology to benefit their progeny. Although several endoparasitoid venom proteins have been identified, little is known about the components of ectoparasitoid venom. To characterize the protein composition of Torymus sinensis Kamijo (Hymenoptera: Torymidae) venom, we used an integrated transcriptomic and proteomic approach and identified 143 venom proteins. Moreover, focusing on venom gland transcriptome, we selected additional 52 transcripts encoding putative venom proteins. As in other parasitoid venoms, hydrolases, including proteases, phosphatases, esterases, and nucleases, constitute the most abundant families in T. sinensis venom, followed by protease inhibitors. These proteins are potentially involved in the complex parasitic syndrome, with different effects on the immune system, physiological processes and development of the host, and contribute to provide nutrients to the parasitoid progeny. Although additional in vivo studies are needed, initial findings offer important information about venom factors and their putative host effects, which are essential to ensure the success of parasitism. |
| format |
article |
| author |
Carmen Scieuzo Rosanna Salvia Antonio Franco Marco Pezzi Flora Cozzolino Milvia Chicca Chiara Scapoli Heiko Vogel Maria Monti Chiara Ferracini Pietro Pucci Alberto Alma Patrizia Falabella |
| author_facet |
Carmen Scieuzo Rosanna Salvia Antonio Franco Marco Pezzi Flora Cozzolino Milvia Chicca Chiara Scapoli Heiko Vogel Maria Monti Chiara Ferracini Pietro Pucci Alberto Alma Patrizia Falabella |
| author_sort |
Carmen Scieuzo |
| title |
An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components |
| title_short |
An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components |
| title_full |
An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components |
| title_fullStr |
An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components |
| title_full_unstemmed |
An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components |
| title_sort |
integrated transcriptomic and proteomic approach to identify the main torymus sinensis venom components |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/fffcd1ef123041afa08ff173ef12fcb7 |
| work_keys_str_mv |
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