El gen tax del virus linfotrópico humano tipo I en la identificación etiológica de la paraparesia espástica tropical: Estudio clínico, serológico y de polimerasa en cadena en 72 pacientes

Background: Tropical spastic paraparesis (TSP) is an endemic disease in Chile. In most countries, only 50% of patients are seropositive to HTLV-I. However, new studies suggest that seronegative TSP is also associated with HTLV-I. Aim: To describe cLinical and virological features of seronegative pat...

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Autores principales:	Cartier R,Luis, Ramírez V,Eugenio, Galeno A,Héctor
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 1999
Materias:	HTLV-I tax gene in TSP HTLV-I antigens Tropical spastic paraparesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98871999000800008
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spelling	oai:scielo:S0034-988719990008000082005-11-14El gen tax del virus linfotrópico humano tipo I en la identificación etiológica de la paraparesia espástica tropical: Estudio clínico, serológico y de polimerasa en cadena en 72 pacientesCartier R,LuisRamírez V,EugenioGaleno A,Héctor HTLV-I tax gene in TSP HTLV-I antigens Tropical spastic paraparesis Background: Tropical spastic paraparesis (TSP) is an endemic disease in Chile. In most countries, only 50% of patients are seropositive to HTLV-I. However, new studies suggest that seronegative TSP is also associated with HTLV-I. Aim: To describe cLinical and virological features of seronegative patients with TSP. Patients and methods: Seventy two Chilean patients with TSP, studied by cLinical, radiological and laboratory methods during 1998, are reported. The determination of antibodies to HTLV-I was accompLished by ELiSA, immunofluorescence and Western-blot analysis. Polymerase chain reaction for tax and 5'Ltr genes was made using primers SK 43-44, LTR1 and LTR6. Results: Thirty one patients were HTLV-I positive and 41 were negative. No cLinical, radiological or laboratory differences were observed between both groups. In seropositive patients, tax and 5'ltr viral gene sequences of the HTLV-I provirus were detected in DNA of peripheral blood mononuclear cells. In seronegative cases, sequences of tax gene were detected, exclusively, in 18 of 41 patients. Conclusions: These results confirm an association with HTLV-I infection in 43,9% of the TSP seronegative patients. These findings support the hypothesis that a defective provirus infects peripheral blood mononuclear cells in seronegative cases of TSP. The importance tax gene in the diagnosis of the TSP is also emphasized.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.127 n.8 19991999-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98871999000800008es10.4067/S0034-98871999000800008
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	HTLV-I tax gene in TSP HTLV-I antigens Tropical spastic paraparesis
spellingShingle	HTLV-I tax gene in TSP HTLV-I antigens Tropical spastic paraparesis Cartier R,Luis Ramírez V,Eugenio Galeno A,Héctor El gen tax del virus linfotrópico humano tipo I en la identificación etiológica de la paraparesia espástica tropical: Estudio clínico, serológico y de polimerasa en cadena en 72 pacientes
description	Background: Tropical spastic paraparesis (TSP) is an endemic disease in Chile. In most countries, only 50% of patients are seropositive to HTLV-I. However, new studies suggest that seronegative TSP is also associated with HTLV-I. Aim: To describe cLinical and virological features of seronegative patients with TSP. Patients and methods: Seventy two Chilean patients with TSP, studied by cLinical, radiological and laboratory methods during 1998, are reported. The determination of antibodies to HTLV-I was accompLished by ELiSA, immunofluorescence and Western-blot analysis. Polymerase chain reaction for tax and 5'Ltr genes was made using primers SK 43-44, LTR1 and LTR6. Results: Thirty one patients were HTLV-I positive and 41 were negative. No cLinical, radiological or laboratory differences were observed between both groups. In seropositive patients, tax and 5'ltr viral gene sequences of the HTLV-I provirus were detected in DNA of peripheral blood mononuclear cells. In seronegative cases, sequences of tax gene were detected, exclusively, in 18 of 41 patients. Conclusions: These results confirm an association with HTLV-I infection in 43,9% of the TSP seronegative patients. These findings support the hypothesis that a defective provirus infects peripheral blood mononuclear cells in seronegative cases of TSP. The importance tax gene in the diagnosis of the TSP is also emphasized.
author	Cartier R,Luis Ramírez V,Eugenio Galeno A,Héctor
author_facet	Cartier R,Luis Ramírez V,Eugenio Galeno A,Héctor
author_sort	Cartier R,Luis
title	El gen tax del virus linfotrópico humano tipo I en la identificación etiológica de la paraparesia espástica tropical: Estudio clínico, serológico y de polimerasa en cadena en 72 pacientes
title_short	El gen tax del virus linfotrópico humano tipo I en la identificación etiológica de la paraparesia espástica tropical: Estudio clínico, serológico y de polimerasa en cadena en 72 pacientes
title_full	El gen tax del virus linfotrópico humano tipo I en la identificación etiológica de la paraparesia espástica tropical: Estudio clínico, serológico y de polimerasa en cadena en 72 pacientes
title_fullStr	El gen tax del virus linfotrópico humano tipo I en la identificación etiológica de la paraparesia espástica tropical: Estudio clínico, serológico y de polimerasa en cadena en 72 pacientes
title_full_unstemmed	El gen tax del virus linfotrópico humano tipo I en la identificación etiológica de la paraparesia espástica tropical: Estudio clínico, serológico y de polimerasa en cadena en 72 pacientes
title_sort	el gen tax del virus linfotrópico humano tipo i en la identificación etiológica de la paraparesia espástica tropical: estudio clínico, serológico y de polimerasa en cadena en 72 pacientes
publisher	Sociedad Médica de Santiago
publishDate	1999
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98871999000800008
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El gen tax del virus linfotrópico humano tipo I en la identificación etiológica de la paraparesia espástica tropical: Estudio clínico, serológico y de polimerasa en cadena en 72 pacientes

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