Efecto inmunosupresor in vitro de las lipoproteínas de baja densidad
Background: Immune cells participate in the formation of atheromatous plate, however little is known about the effects of native or oxidatively modified lipoproteins on these cells. Aim: To study the effects of lipoproteins on in vitro mononuclear cell proliferation. Material and methods: Peripheral...
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| Lenguaje: | Spanish / Castilian |
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Sociedad Médica de Santiago
1999
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oai:scielo:S0034-988719990011000032005-11-23Efecto inmunosupresor in vitro de las lipoproteínas de baja densidadGonzález R,MargaritaSanz C,IvetteRojas S,NinaSilva F,VíctorKirsten L,LilianBustamante C,Marcelo Atherosclerosis Lipoproteins Lipoproteins, LDL Lymphocyte transformation Background: Immune cells participate in the formation of atheromatous plate, however little is known about the effects of native or oxidatively modified lipoproteins on these cells. Aim: To study the effects of lipoproteins on in vitro mononuclear cell proliferation. Material and methods: Peripheral blood mononuclear cells were obtained from 10 patients with type 2 diabetes mellitus (aged 52 ± 9 years old with a disease duration of 8.2 ± 5.7 years and a mean glycosilated hemoglobin of 9.3 ± 2.2%) and 10 non diabetic healthy controls (aged 50.3 ± 7.1 years old). These were stimulated with phytohemagglutinin (PHA) alone or in the presence of native LDLS, malondialdehyde modified LDLs or glycated LDLs. Proliferation was measured as 3H-thymidine incorporation and expressed as Stimulation Index (SI). Results: SI of patients and healthy subjects, after PHA stimulation were similar: (57.5 ± 29.8 and 61.1 ± 23.5) respectively LDLs did not induce proliferation in neither group. Native LDLs produced a 98% inhibition of PHA induced proliferation. Malondialdehyde modified and glycated LDLs caused a 50% inhibition. The suppressive effect was maintained when lipoproteins were incorporated to culture media 60 min prior or after PHA stimulation. Conclusions: Lipoproteins inhibit in vitro PHA induced peripheral blood mononuclear cell proliferation both in diabetic and in non diabetic subjects.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.127 n.11 19991999-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98871999001100003es10.4067/S0034-98871999001100003 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
Spanish / Castilian |
| topic |
Atherosclerosis Lipoproteins Lipoproteins, LDL Lymphocyte transformation |
| spellingShingle |
Atherosclerosis Lipoproteins Lipoproteins, LDL Lymphocyte transformation González R,Margarita Sanz C,Ivette Rojas S,Nina Silva F,Víctor Kirsten L,Lilian Bustamante C,Marcelo Efecto inmunosupresor in vitro de las lipoproteínas de baja densidad |
| description |
Background: Immune cells participate in the formation of atheromatous plate, however little is known about the effects of native or oxidatively modified lipoproteins on these cells. Aim: To study the effects of lipoproteins on in vitro mononuclear cell proliferation. Material and methods: Peripheral blood mononuclear cells were obtained from 10 patients with type 2 diabetes mellitus (aged 52 ± 9 years old with a disease duration of 8.2 ± 5.7 years and a mean glycosilated hemoglobin of 9.3 ± 2.2%) and 10 non diabetic healthy controls (aged 50.3 ± 7.1 years old). These were stimulated with phytohemagglutinin (PHA) alone or in the presence of native LDLS, malondialdehyde modified LDLs or glycated LDLs. Proliferation was measured as 3H-thymidine incorporation and expressed as Stimulation Index (SI). Results: SI of patients and healthy subjects, after PHA stimulation were similar: (57.5 ± 29.8 and 61.1 ± 23.5) respectively LDLs did not induce proliferation in neither group. Native LDLs produced a 98% inhibition of PHA induced proliferation. Malondialdehyde modified and glycated LDLs caused a 50% inhibition. The suppressive effect was maintained when lipoproteins were incorporated to culture media 60 min prior or after PHA stimulation. Conclusions: Lipoproteins inhibit in vitro PHA induced peripheral blood mononuclear cell proliferation both in diabetic and in non diabetic subjects. |
| author |
González R,Margarita Sanz C,Ivette Rojas S,Nina Silva F,Víctor Kirsten L,Lilian Bustamante C,Marcelo |
| author_facet |
González R,Margarita Sanz C,Ivette Rojas S,Nina Silva F,Víctor Kirsten L,Lilian Bustamante C,Marcelo |
| author_sort |
González R,Margarita |
| title |
Efecto inmunosupresor in vitro de las lipoproteínas de baja densidad |
| title_short |
Efecto inmunosupresor in vitro de las lipoproteínas de baja densidad |
| title_full |
Efecto inmunosupresor in vitro de las lipoproteínas de baja densidad |
| title_fullStr |
Efecto inmunosupresor in vitro de las lipoproteínas de baja densidad |
| title_full_unstemmed |
Efecto inmunosupresor in vitro de las lipoproteínas de baja densidad |
| title_sort |
efecto inmunosupresor in vitro de las lipoproteínas de baja densidad |
| publisher |
Sociedad Médica de Santiago |
| publishDate |
1999 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98871999001100003 |
| work_keys_str_mv |
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| _version_ |
1718435888708452353 |