Aspectos que afectan el análisis de contenido de ADN por citometría de flujo

DNA ploidy and cell cycle analysis by flow cytometry is used to obtain additional information about the diagnosis and prognosis of different types of cancer. However, there are several disagreements among authors about the tissue source (fresh-frozen or paraffin embedded), cellular dissociation meth...

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Autores principales: Castillo N,Juan L, Kawaguchi P,Fernando, Madariaga B,Jaime, Venegas R,Oscar, Lecannelier F,Eduardo, Ocampo R,Sandor, Castillo N,Marcelo
Lenguaje:Spanish / Castilian
Publicado: Sociedad Médica de Santiago 1999
Materias:
DNA
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98871999001100014
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spelling oai:scielo:S0034-988719990011000142005-11-24Aspectos que afectan el análisis de contenido de ADN por citometría de flujoCastillo N,Juan LKawaguchi P,FernandoMadariaga B,JaimeVenegas R,OscarLecannelier F,EduardoOcampo R,SandorCastillo N,Marcelo Cell Cycle Cytological Techniques DNA Ploidies DNA ploidy and cell cycle analysis by flow cytometry is used to obtain additional information about the diagnosis and prognosis of different types of cancer. However, there are several disagreements among authors about the tissue source (fresh-frozen or paraffin embedded), cellular dissociation methods (mechanical, enzymatic or other), use of different dyes, lasers, analysis software with different mathematical models and interpretation of results. A discussion about the different aspects that affect the study of DNA ploidy and cell cycle and a consensus in publications is mandatory. A strict control of analysis processes and data interpretation is also necessary.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.127 n.11 19991999-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98871999001100014es10.4067/S0034-98871999001100014
institution Scielo Chile
collection Scielo Chile
language Spanish / Castilian
topic Cell Cycle
Cytological Techniques
DNA
Ploidies
spellingShingle Cell Cycle
Cytological Techniques
DNA
Ploidies
Castillo N,Juan L
Kawaguchi P,Fernando
Madariaga B,Jaime
Venegas R,Oscar
Lecannelier F,Eduardo
Ocampo R,Sandor
Castillo N,Marcelo
Aspectos que afectan el análisis de contenido de ADN por citometría de flujo
description DNA ploidy and cell cycle analysis by flow cytometry is used to obtain additional information about the diagnosis and prognosis of different types of cancer. However, there are several disagreements among authors about the tissue source (fresh-frozen or paraffin embedded), cellular dissociation methods (mechanical, enzymatic or other), use of different dyes, lasers, analysis software with different mathematical models and interpretation of results. A discussion about the different aspects that affect the study of DNA ploidy and cell cycle and a consensus in publications is mandatory. A strict control of analysis processes and data interpretation is also necessary.
author Castillo N,Juan L
Kawaguchi P,Fernando
Madariaga B,Jaime
Venegas R,Oscar
Lecannelier F,Eduardo
Ocampo R,Sandor
Castillo N,Marcelo
author_facet Castillo N,Juan L
Kawaguchi P,Fernando
Madariaga B,Jaime
Venegas R,Oscar
Lecannelier F,Eduardo
Ocampo R,Sandor
Castillo N,Marcelo
author_sort Castillo N,Juan L
title Aspectos que afectan el análisis de contenido de ADN por citometría de flujo
title_short Aspectos que afectan el análisis de contenido de ADN por citometría de flujo
title_full Aspectos que afectan el análisis de contenido de ADN por citometría de flujo
title_fullStr Aspectos que afectan el análisis de contenido de ADN por citometría de flujo
title_full_unstemmed Aspectos que afectan el análisis de contenido de ADN por citometría de flujo
title_sort aspectos que afectan el análisis de contenido de adn por citometría de flujo
publisher Sociedad Médica de Santiago
publishDate 1999
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98871999001100014
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